YANG Yanmin,WEI Yongxiang,LIU Cheng,et al.Tissue Culture and Virus Elimination Technique for Apple Dwarf Rootstock[J].Northern Horticulture,2016,40(04):107-112.[doi:10.11937/bfyy.201604028]
苹果矮化砧木组织培养及脱病毒技术
- Title:
- Tissue Culture and Virus Elimination Technique for Apple Dwarf Rootstock
- Keywords:
- apple; dwarf rootstock; tissue culture; rapid propagation; virus elimination
- 文献标志码:
- A
- 摘要:
- 以“马克9”、“辽砧2号”、“GM256”3个辽宁地区常用的苹果矮化砧木品种为试材，研究了苹果矮化砧木组织培养及试管苗热处理脱病毒技术，以建立无病毒苹果矮化砧木苗快繁体系。结果表明：对于品种“马克9”，最佳诱导、增殖和生根培养基分别为MS+6-BA 1.5 mg/L+NAA 0.1 mg/L、6-BA 0.8 mg/L+NAA 0.1 mg/L和1/2MS+0.5 IBA mg/L+0.3 NAA mg/L，诱导率为85.7%；对于品种“辽砧2号”，最佳诱导和增殖培养基分别为MS+6-BA 1.5 mg/L+NAA 0.1 mg/L和 6-BA 0.5 mg/L+NAA 0.1 mg/L，诱导率为87.5%，最佳生根培养基同“马克9”；对于品种“GM256”，最佳诱导和增殖培养基分别为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L和 6-BA 0.8 mg/L+NAA 0.1 mg/L，诱导率为78.5%，最佳生根培养基同“马克9”；以“马克9”耐热性最强，其次是“GM256”和“辽砧2号”；热处理40 d脱除病毒效果最好，脱病毒率分别为53.33%、66.67%、71.43%；海藻素1 000倍液处理5 min时，3个砧木品种生根试管苗田间移栽的成活率最好，分别是93.33%、92.00%和87.50%。
- Abstract:
- Taking ‘Mark 9’,‘Liaozhen No.2 ’and ‘GM256’ apple dwarf rootstock varieties which were commonly used in Liaoning as materials，the technique of tissue culture and heat treatment were studied to establish a rapid propagation system for virus-free apple dwarf rootstock.The results showed that for ‘Mark 9’，MS+6-BA 1.5 mg/L+NAA 0.1 mg/L，6-BA 0.8 mg/L+NAA 0.1 mg/L and 1/2MS+IBA 0.5 mg/L+NAA 0.3 mg/L were the best media for inducing，proliferating and rooting，respectively，induction rate was 85.7%；for ‘Liaozhen No.2’，MS+6-BA 1.5 mg/L+NAA 0.1 mg/L and 6-BA 0.5 mg/L+NAA 0.1 mg/L were the best media for inducing and proliferating，respectively，induction rate was 87.5%，the rooting medium same as ‘Mark 9’；for ‘GM256’，MS+6-BA 1.0 mg/L+NAA 0.1 mg/L and 6-BA 0.8 mg/L+NAA 0.1 mg/L were the best media for inducing and proliferating，respectively，induction rate was 78.5%，the rooting medium same as ‘Mark 9’.The heat resistance of ‘Mark 9’ was the strongest，followed by the ‘GM256’ and ‘Liaozhen No.2’.The effect of virus elimination was the best with heat treatment for 40 days，the virus-free rate were 53.33%，66.67% and 71.43% for ‘Mark 9’，‘Liaozhen No.2’ and ‘GM256’，respectively.Treated for 5 minutes by 1 000×cytex，the survival rate of transplant were the highest，of which 93.33%,92.00%,87.50% for three varieties tested，respectively.
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备注/Memo
第一作者简介：杨艳敏（1975-），女，本科，副研究员，现主要从事果树组织培养及生物育种等研究工作。E-mail：yymzcb@163.com.责任作者：魏永祥（1962-），男，本科，研究员，现主要从事小浆果栽培与育种等研究工作。E-mail：lgwdzxw@163.com.