GUAN Wenfang,DAI Xiangqun,HU Qiang,et al.Biocontrol Effect of Bio-fertilizer ‘Nanjing Shield’ on Strawberry[J].Northern Horticulture,2016,40(02):158-162.[doi:10.11937/bfyy.201602043]
微生物肥料“宁盾”对草莓的促生防病效果初探
- Title:
- Biocontrol Effect of Bio-fertilizer ‘Nanjing Shield’ on Strawberry
- 文献标志码:
- A
- 摘要:
- 以草莓为试材,在其移栽时使用微生物肥料“宁盾”灌根处理,研究了“宁盾”对草莓的生长状况、产量及果实的品质的影响。结果表明:移栽43 d后,使用“宁盾”灌根处理组的花朵数为4.8朵/单株,比对照组提高3.3朵/单株;“宁盾”对草莓枯萎病和炭疽病的生防效果分别达74.46%和72.18%。成熟期处理组果实的平均单果重较对照组提高8.8 g;同时,处理组草莓果实的可溶性蛋白质、维生素C、可溶性糖等含量显著优于对照组。
- Abstract:
- Taking strawberry as test material,the effect of bio-fertilizer ‘Nanjing Shield’ on the growth of strawberry and the bio-control of strawberry diseases were studied,by inoculation while strawberry was transplanted.The results showed that the number of flowers of ‘Nanjing Shield’ treatment was 4.8 per plant and was increased by 3.3 per plant,compared with control 43 days after inoculation.And the average weight of fruit was also raised by 8.8 g per fruit in mature period.Meanwhile,the contents of soluble protein,vitamin C,and soluble sugar of fruit of ‘Nanjing Shield’ treatment were significantly higher than control.The bio-control effect of ‘Nanjing Shield’ on strawberry blight and anthracnose reached 74.46% and 72.18%.
参考文献/References:
[1]冯坤蓉,金芳,周忠雄,等.梭伦剥管菌对草莓生长,生理生化特性,品质及产量的影响[J].甘肃农业大学学报,2014(1):79-82. [2]尹宝重,刘雪静,高峰,等.不同AM真菌对草莓生长及生理的影响[J].中国农学通报,2009,25(16):173-176. [3]MOUBAYIDIN L,DI MAMBRO R,SABATINI S.Cytokinin-auxin crosstalk[J].Trends in Plant Science,2009,14(10):557-562. [4]OVERVOORDE P,FUKAKI H,BEECKMAN T.Auxin control of root development [J].Cold Spring Harbor Perspectives in Biology,2010,2(6):a001537. [5]STEPANOVA A N,ALONSO J M.Ethylene signaling and response:where different regulatory modules meet [J].Current Opinion in Plant Biology,2009,12(5):548-555. [6]邢卫峰,于侦云,陈刘军,等.生物肥料“宁盾”对甜瓜枯萎病的防治效果[J].江苏农业科学,2014(3):78-81. [7]GUO J H,LIU H X,LI S M.PSX combination,a biocontrol bacterium combination that can prevent and control soil-borne diseases of a variety of crops[P].European Patent,No.2255660.2012. [8]王奎萍,周冬梅,刘苏闽,等.宁盾一号菌剂不同处理方法对番茄生长的影响[J].广东农业科学,2013,40(23):61-64. [9]葛红莲,郭坚华,祁红英,等.复合菌剂AR99防治辣椒青枯病[J].植物病理学报,2004,34(2):162-165. [10]郭亚辉,卢月霞,郭坚华.生物农药防治辣椒疫病的效果[J].安徽农业科学,2007,35(4):1065-1067. [11]齐爱勇,赵绪生,刘大群.芽孢杆菌生物防治植物病害研究现状[J].中国农学通报,2011,27(12):277-280. [12]韩涛,章淑艳.生物复合草莓抗重茬剂在草莓上的应用效果[J].河北省科学院学报,2014,31(1):57-60. [13]NAM M H,PARK M S,KIM H G,et al.Biological control of strawberry Fusarium wilt caused by Fusarium oxysporum f.sp.fragariae using Bacillus velezensis BS87 and RK1 formulation [J].Journal of Microbiology and Biotechnology,2009,19(5):520-524. [14]DELP B R,MILHOLLAND R D.Evaluating strawberry plants for resistance to Colletotrichumfragariae [J].Plant Disease,1980,64(12):1071-1073. [15]BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1):248-254. [16]李合生.植物生理生化实验原理与技术(面向21世纪课程教材)[M].北京:高等教育出版社,2000. [17]陈秀莲,魏晓琼,丛艳静.炬荣复合微生物肥料在蕹菜上的应用研究[J].中国果菜,2014,34(8):61-63. [18]张洪胜,杨述.复合微生物肥的主要功效与作用机理[J].烟台果树,2014(3):1-2. [19]NADEEM S M,AHMAD M,ZAHIR Z A,et al.The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments [J].Biotechnology Advances,2014,32(2):429-448. [20]刘丹,张丽萍,史延茂,等.生防细菌对植物根围微生态效应的研究进展[J].中国农学通报,2014,30(7):260-265. [21]吴兴兴,吴毅歆,赵正龙,等.4个PGPR菌株拌种对干旱条件下蚕豆生长及产量的影响[J].干旱区研究,2012,29(2):203-207. [22]KLOEPPER J W,LIFSHITZ R,ZABLOTOWICZ R M.Free-living bacterial inocula for enhancing crop productivity[J].Trends in Biotechnology,1989,7(2):39-44. [23]陈刘军,俞仪阳,王超,等.蜡质芽孢杆菌AR156防治水稻纹枯病机理初探[J].中国生物防治学报,2014,30(1):107-112. [24]刘苏闽,周冬梅,杨敬辉,等.复合菌剂对草莓黄萎病的田间防治效果[J].中国生物防治,2010,26(4):501-503. [25]CHEN Y,YAN F,CHAI Y R,et al.Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation [J].Environmental Microbiology,2013,15(3):848-864. [26]高喜叶,郭永明,曹兴明,等.新型生物菌肥对日光温室草莓果实糖酸含量的影响[J].北方园艺,2014(17):54-56.
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备注/Memo
第一作者简介:管文芳(1988-),女,硕士,研究方向为农作物病虫害的防治。E-mail:672585347@qq.com. 责任作者:郭坚华(1966-),女,博士,教授,博士生导师,研究方向为植物病害的生物防治。E-mail:jhguo@njau.edu.cn. 基金项目:江苏省农业三新工程资助项目(SXGC[2014]305)。