HUANG Wanbing,GUI Yang,GONG Guanglu,et al.Research on Intracellular Polysaccharide Yield and Extracellular Enzyme Activity Change of Armillarilla Strains Isolated From Guizhou[J].Northern Horticulture,2016,40(02):134-138.[doi:10.11937/bfyy.201602035]
七株贵州蜜环菌胞内多糖得率及胞外酶活性研究
- Title:
- Research on Intracellular Polysaccharide Yield and Extracellular Enzyme Activity Change of Armillarilla Strains Isolated From Guizhou
- 关键词:
- 蜜环菌; 木聚糖酶; 羧甲基纤维素酶（CMC酶）; 漆酶; 胞内多糖
- Keywords:
- Armillarilla; xylanase; CMCase; laccase; intracellular polysaccharide
- 文献标志码:
- A
- 摘要:
- 以7株贵州蜜环菌为试材，采用苯酚-硫酸法测定多糖得率、DNS法测定蜜环菌发酵液中木聚糖酶和羧甲基纤维素酶的活性、丁香醛连氮测定发酵液中漆酶活性，研究了不同蜜环菌在发酵过程中漆酶等胞外酶活性变化规律及菌丝胞内多糖提取率。结果表明：各菌株间多糖得率存在显著差异；各菌株胞外酶活性变化趋势均是先升后降；各酶在不同菌株间达到最大酶活的时间、最大酶活性值存在明显差异；优良菌株DJ1第4天就能结束发酵，达到最大酶活时间最短、持续时间长。蜜环菌液体发酵时最早分泌的是CMC酶、漆酶，然后是木聚糖酶，但起主要作用的是木聚糖酶。
- Abstract:
- Taking Armillarilla strains isolated from Guizhou as test material，using phenol-sulfuric acid method to determine the content of total polysaccharide，the DNS method and syringaldazine were applied to measure the enzyme activity of xylanase，carboxymethyl cellulose enzyme and laccase，the change rule of extracellular enzyme activity and intracellular polysaccharide yield of different Armillarilla hyphae came from liquid fermentation were studied.The results showed that，there were significant differences between polysaccharide yield of different strains.The change rule of the extracellular different enzyme activity were same.They all rose at first and lowered later.The maximum enzyme activity and the time when enzyme activity reach maximum between different strains were also obvious difference.The fine strain DJ1 could end fermentation on the fourth day.Its maximum enzyme activity was always the earliest to appear and at the latest to disappear.When liquid fermentation，Armillaria prior secreted CMC enzyme and laccase，then secreted xylanase.But xylanase played a main role.
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备注/Memo
第一作者简介：黄万兵(1988-)，男，湖北荆州人，硕士，研究实习员，研究方向为应用微生物。E-mail:huangwb1012@qq.com. 责任作者：朱国胜（1971-），男，博士，研究员，硕士生导师，现主要从事中药材及食药用真菌等研究工作。E-mail:zgsah@163.com. 基金项目：贵州省优秀青年科技人才培养对象专项资金资助项目（黔科合人字（2011）35号）；贵州省科技计划资助项目（黔科合院所创能（2010）4002）；中央补助地方科技基础条件专项基金资助项目（黔科条中补地（2010）4002）；贵州省中药现代化科技产业研究开发专项资助项目（黔科合中药字[2011]5035号）；贵州省农业科学院研究生创新基金资助项目（黔农科合（创新基金）2011016号）；贵州省农业科学院专项资金资助项目（黔农科院院专项[2012]020号，黔农科院院专项[2014]035号）；贵州省农业科学院博士科研启动资助项目（黔农科院人才启动项目[2009]006号）。