LI Bingbing,WEN Zhiqiang.Pathogenicity Mechanism of Yang Mei Shuang Disease on Tremella fuciformis and Inhibition Spectrum of the Pathogenic Bacterium[J].Northern Horticulture,2015,39(24):114-117.[doi:10.11937/bfyy.201524032]
银耳杨梅霜病发病机理及其病原菌抗菌谱的测定
- Title:
- Pathogenicity Mechanism of Yang Mei Shuang Disease on Tremella fuciformis and Inhibition Spectrum of the Pathogenic Bacterium
- 文献标志码:
- A
- 摘要:
- 以银耳杨梅霜病发病菌筒为试材，采用平板培养及分子生物学方法，研究了杨梅霜病病原菌的生物学特性及抗菌谱。结果表明：分离到3株病原菌Js-1T、Js-2、Js-3的形态特征和培养特性相同，16S rDNA酶切带型完全一致，三者为重复菌株；回接试验证实Js-1T菌株为杨梅霜病的病原菌；对峙培养试验表明，Js-1T菌株产生的抗菌物质抑制香灰菌和银耳菌丝的生长；16S rDNA序列分析表明，Js-1T菌株与链霉菌属菌株Streptomyces hebeiensis strain NBRC 101006T的同源性最高为97.62%。结合形态特征和分子生物学分析，证明Js-1T菌株属于链霉菌属。同时测定了Js-1T菌株的抗菌谱，其对大肠杆菌的抑菌圈直径为2.76 cm，对枯草芽孢杆菌的抑菌圈直径为1.26 cm；对真菌核盘菌、有害疣孢霉菌、稻瘟菌、木霉、根霉的抑菌圈直径分别为4.29、4.07、3.85、3.37、2.90 cm。此外，对镰刀菌和香蕉枯萎病菌天蓝一号也具有一定的抑制作用。
- Abstract:
- Taking Tremella fuciformis artificial bed-log which was infected with Yang mei shuang disease as materials，plate cultivation and molecular biology methods were used to study the biological characteristics and antibacterial spectrum of pathogenic bacterium.The results showed that the colonies characteristics and cultural characteristics of the three pathogen strains Js-1T，Js-2 and Js-3 were the same as PDA culture.And the 16S rDNA of three strains had the same bands after digestion.，so these strains were repeated strains.Strain Js-1T was confirmed to be the pathogen of this disease through Koch’s rules.Strain Js-1T had strong inhibition on xiang hui and tremella gemma，which could be the cause of the disease.16S rDNA squence analysis showed that strain Js-1T had the highest similarity with Streptomyces hebeiensis strain NBRC 101006T，reaching 97.62%.The results of cultural characteristics and molecular biological analysis showed that strain Js-1T belonged to Streptomyces.What’s more，the antibacterial activity of strain Js-1T was determined.The results showed that strain Js-1T had strong inhibition against these tested strains.The diameters of Escherichia coli and Bacillus subtilis were 2.76 cm and 1.26 cm,respectively.The diameters of Sclerotinia sclerotiorum，Mycogone perniciosa，Pyricularia grisea，Trichoderma viride，Rhizopus stolonir were 4.29，4.07，3.85，3.37 and 2.90 cm,respectively.In addition，strain Js-1T had antibacterial activity to Fusarium spp and Banana vasicular wilt.
参考文献/References:
[1]张海风.感病银耳中微生物的T-RFLP分析[D].福州：福建农林大学,2011. [2]林成辉,唐乐尘.白木耳“杨梅生病”和“死菌”原因探讨[J].中国食用菌,2000,19(2):23. [3]戴维浩,戴伟鸿.银耳杨梅霜病的污染现象和防治方法[J].浙江食用菌,2010,18(2):48. [4]张蕾,张金涛,杨文博,等.链霉菌 Z314 的分类鉴定及其转化产物普伐他汀的纯化研究[J].微生物学报,2008,48(1):33-37. [5]ALTSCHUL S F，MADDEN T L，SCHFFER A A，et al.Gapped BLAST and PSIBLAST：a new generation of protein database search programs[J].Nucleic Acids Res,1997，25：3389-3402. [6]THOMPSON J D，GIBSON T J，PLEWNIAK F，et al.The ClustalX windows interface：flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Res,1997，24：4876-4882. [7]杨顺锦,詹儒林,柳凤,等.金沙江干热河谷地区芒果畸形病的病原菌[J].菌物学报,2011,30(1):27-31. [8]疏秀林,安德荣,张勤福,等.土壤拮抗放线菌S2521026的筛选及其初步分类鉴定[J].西北农林科技大学学报(自然科学版)，2004,32(12):57-60. [9]YARZA P,RICHTER M,PEPLIES J,et al.The all-species living tree project:a 16S rRNA-based phylogenetic tree of all sequenced type strains[J].Syst Appl Microbiol,2008，31：241-250. [10]STACKEBRANDT E,GOEBEL B M.Taxonomic note：a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J].Int J Syst Bacteriol,1994，44:846-849. [11]曹广丽.番茄叶霉菌拮抗放线菌的筛选、鉴定及发酵条件研究[D].哈尔滨：东北农业大学,2006. [12]何鸿洪.番茄青枯病拮抗放线菌的筛选研究[D].南宁：广西大学,2006. [13]隋丽.放线菌769对水稻抗稻瘟病的诱导抗性研究[D].长春：吉林农业大学,2008. [14]刘丽玲.人参锈腐病颉颃放线菌发酵条件及抗生素分离提取[D].长春：吉林农业大学,2008. [15]何娜.五指山放线菌分离菌株活性测定及WZ254菌株产生的抗生素分离纯化[D].海口：海南大学,2008.
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备注/Memo
第一作者简介：李兵兵（1986-），男，江苏淮安人，硕士，研究方向为应用微生物学与基因工程。E-mail:leeb1002@163.com.责任作者：温志强（1964-），男，本科，教授，研究方向为食用菌及病虫害防治。E-mail:546650546@qq.com.基金项目：国家支撑计划资助项目（2013BAD16B03）。