ZHENG Xizi,YUAN Liuxiang,ZHAO Bing,et al.Study on Rapid Propagation Technology of Seeds Tissue Culture of Rhododendron calophytum Franch in Qinling[J].Northern Horticulture,2015,39(24):75-79.[doi:10.11937/bfyy.201524022]
秦岭美容杜鹃种子组培快繁技术研究
- Title:
- Study on Rapid Propagation Technology of Seeds Tissue Culture of Rhododendron calophytum Franch in Qinling
- Keywords:
- Rhododendron calophytum Franch; seeds; tissue culture
- 文献标志码:
- A
- 摘要:
- 以秦岭地区野生美容杜鹃种子为外植体，研究了外植体消毒时间与2种不同基本培养基初代培养，以及不同激素浓度对增殖培养和生根培养的影响。结果表明：种子消毒时间3 min或者更长时间为好；在加入1.5 mg/L ZT和0.5 mg/L NAA的WPS培养基中发现不定芽繁殖系数达到4.0，且丛生芽长势良好；在1/2 WPM中生根培养，30 d生根率均达到100%，且试管苗生长健壮，根系发达。
- Abstract:
- In this study,wild Rhododendron calophytum seeds in the Qinling Mountains area were used as explants, the effect of disinfection time,two kinds of basic initial medium,hormone concentrations on the proliferation and rooting culture were explored.The results showed that the best disinfection time of seeds was 3 minutes or longer.Propagation coefficient of adventitious bud reached 4.0 in the WPS medium with 1.5 mg/L ZT and 0.5 mg/L NAA，and cluster buds grew well.Rooting culture in 1/2 WPM，after 30 days，the rooting rate reached 100%.And plantlets grew strong，roots developed well.
参考文献/References:
[1]胡琳贞，方明渊，中国植物志[M].北京：北京科学出版社,1994:107-339. [2]赵冰，张果，司国臣，等.秦岭野生杜鹃花属植物种质资源调查研究[J].西北林学院学报,2013，28(1)：104-109. [3]司国臣，张延龙，顾欣，等.秦岭野生美容杜鹃扦插繁殖技术[J].北方园艺，2012(3)：77-79. [4]苗永美，王永清，庄平，等.大萼杜鹃的快速繁殖研究[J].中国林副特产，2007(4)：4. [5]苗永美，王永清，庄平，等.大树杜鹃组织培养技术研究[J].安徽科技学院学报，2007(6)：23-26. [6]崔丽华.植物生长调节物质对组织培养中不定芽不定根的作用[J].辽宁师专学报(自然科学版)，2000(2)：97-99. [7]李天兴，张超.美容杜鹃栽培技术初步研究[J].四川林业科技，2005，26(1)：62-64. [8]罗彭，庄平，白洁.大白杜鹃、美容杜鹃和喇叭杜鹃的组织培养[J].植物生理学通讯，2007(2):43，326. [9]刘燕，王济红，陈训，等.大白杜鹃种子胚组织培养研究[J].贵州农业科学，2010(12)：30-33. [10]肖东梅，宣艳辉，朱光杰，等.三花杜鹃组培快繁技术的研究[J].北方园艺，2010(23)：140-142. [11]陈平芬，刘家迅，李兴贵，等.文雅杜鹃组织培养研究[J].北方园艺，2011(17)：134-136. [12]罗琳，白洁，陈超，等.美容杜鹃叶片再生及愈伤组织耐热生理研究[J].西北植物学报，2014(7)：1377-1382.
相似文献/References:
[1]田英翠,曹受金,曹基武.六种榉树种子形态特征及萌发特性的研究[J].北方园艺,2014,38(11):71.
TIAN Ying-cui,CAO Shou-jin,CAO Ji-wu.Study on Morphology and Germination Characteristics of Seeds of Six Species of Zelkova schneideriana [J].Northern Horticulture,2014,38(24):71.
[2]杨 凯,孙迎坤,谭 雯,等.盐胁迫对香石竹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(22):86.
YANG Kai,SUN Ying-kun,TAN Wen,et al.Effects of Salt Stress on Seeds Germination and Seedling Growth of Carnation[J].Northern Horticulture,2013,37(24):86.
[3]马子林.藏药马尿泡种子吸水特性及抗旱性的初步研究[J].北方园艺,2013,37(22):164.
MA Zi-lin.Preliminary Study on Water Absorbing Character and Drought Resistance of the Seed of Tibetan Medicine Przewalskia tangutica Maxim[J].Northern Horticulture,2013,37(24):164.
[4]徐小玉,张凤银,张方俊.氯化钠胁迫对雏菊种子萌发及幼苗生长的影响[J].北方园艺,2014,38(14):52.
XU Xiao-yu,ZHANG Feng-yin,ZHANG Fang-jun.Effect of NaCl Stress on Seed Germination and Seedling Growth of Bellis perennis[J].Northern Horticulture,2014,38(24):52.
[5]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(24):1.
[6]张 芹,徐学山,李保会,等.不同处理条件对暴马丁香种子萌发的影响[J].北方园艺,2014,38(01):54.
ZHANG Qin,XU Xue-shan,LI Bao-hui,et al.Effects of Different Treatments Conditions on the Germination of Syringa reticulatassp. amurensisSeeds[J].Northern Horticulture,2014,38(24):54.
[7]崔霞,梁燕,王玲慧.不同浓度化学诱变剂甲基磺酸乙酯对番茄种子发芽的影响[J].北方园艺,2013,37(14):28.
CUI Xia,LIANG Yan,WANG Ling-hui.Effect of Different Concentrations of Chemical Mutagen EMS on Tomato Seed Germination[J].Northern Horticulture,2013,37(24):28.
[8]仲莎,姜守阳,刘桂花,等.化学药剂处理对黑籽南瓜种子发芽率的影响[J].北方园艺,2013,37(14):34.
ZHONG Sha,JIANG Shou-yang,LIU Gui-hua,et al.Effect of Chemicals on the Germination Rate of Cucurbita ficifolia Bauche Seeds[J].Northern Horticulture,2013,37(24):34.
[9]杨国会,孙立梅,邢力,等.不同层积处理方法对东北红豆杉种子后熟过程中贮藏物质的影响[J].北方园艺,2013,37(14):82.
YANG Guohui,SUN Limei,XING Li,et al.Effects of Different Concentrations of GA3 with Different Temperature Stratifications on the Storage Substance Changes of Taxus cuspidata Sieb.et Zucc.[J].Northern Horticulture,2013,37(24):82.
[10]许耀照,吕彪,王勤礼,等.沙葱种子发芽特性研究[J].北方园艺,2014,38(06):4.
XU Yao-zhao,LV Biao,WANG Qin-li,et al.Study on Germination Character of Allium mongolicum Regel.[J].Northern Horticulture,2014,38(24):4.
备注/Memo
第一作者简介：郑茜子（1990-），女，河北唐山人，硕士研究生，研究方向为杜鹃色素分析与组织培养。E-mail：287096413@qq.com.责任作者：赵冰（1980-），女，博士，副教授，硕士生导师，现主要从事园林植物资源与应用研究及教学等工作。E-mail：bingbing2003915@163.com.基金项目：西北农林科技大学国际科技合作基金资助项目 (A213021501)。