LI Duofang,TIAN Anran,GENG Jinpeng,et al.Biological Effect of Zinnia elegans Jacq.Induced by Space Flight in SP1 Generation[J].Northern Horticulture,2015,39(23):97-100.[doi:10.11937/bfyy.201523027]
航天搭载诱导百日草的生物学效应
- Title:
- Biological Effect of Zinnia elegans Jacq.Induced by Space Flight in SP1 Generation
- Keywords:
- space flight; Zinnia elegans Jacq.; phyllotaxy; RAPD
- 文献标志码:
- A
- 摘要:
- 以百日草种子为试材，通过生物统计和分子标记的方法，对航天搭载处理的百日草干种子当代植株生长状况进行了研究。结果表明：航天搭载处理使百日草种子的发芽率有所下降；而航天搭载处理组的百日草当代幼苗黑斑病的感染率有一定升高；在航天搭载处理组的变异植株中发现1株稳定的三叶共生变异株H1-2，变异株的叶片明显小于对照植株的叶片。随机扩增多态性DNA（RAPD）分析表明，与对照植株相比H1-2基因组的变异率为13.4%。航天搭载诱导百日草生物学效应的研究为花卉的航天诱变育种提供了一定的参考依据。
- Abstract:
- Using the seeds of Zinna elegans Jacq.as the test materials，the growth and development of plants induced by space flight in SP1 generation were investigated with the methods of biostatistics and molecular marker.The results showed that after experiencing space flight，the germination rate of Zinnia decreased slightly，and the infection rate of the black spot of Zinnia increased.In the space flight groups，a stable clover symbiotic variant H1-2 was found.The leaf size of the variant H1-2 was obviously less than that of the wild plant.Random amplified polymorphic DNA (RAPD) analysis showed that compared with the control plants，the genomic variation rate of the variant H1-2 was 13.4%.The studies of the mutagenic effects of space flight on Zinnia elegans Jacq.provided an experimental basis for space mutation breeding of ornamental plants.
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备注/Memo
第一作者简介：李多芳（1985-），女，河北邯郸人，博士研究生，现主要从事大分子动力学与辐射生物学和物种进化等研究工作。E-mail：duofang_6608@163.com.责任作者：展永（1954-），男，教授，博士生导师，研究方向为大分子动力学离子与通道及辐射生物学和分子马达。E-mail：yongz2013@163.com.基金项目：河北省自然科学基金资助项目（C2013202192）。