TANG Doudou,LI Houhua,ZHANG Yanlong,et al.Analysis on Pigments and Antioxidant Activity in Red-leaf of Paeonia rocki var. purpurea[J].Northern Horticulture,2015,39(23):79-86.[doi:10.11937/bfyy.201523023]
变异紫斑牡丹红色叶色素与抗氧化活性分析
- Title:
- Analysis on Pigments and Antioxidant Activity in Red-leaf of Paeonia rocki var. purpurea
- Keywords:
- Paeonia rockii; red-leaf; peonidin-3; 5-di-O-glucoside; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以紫斑牡丹和变异紫斑牡丹的叶片为试材，采用高效液相色谱、紫外-可见分光光度法、薄层色谱对变异紫斑牡丹红色叶和紫斑牡丹绿色叶的色素进行定性定量分析；采用1,1-二苯基-2-三硝基苯肼自由基和2,2’-联氮-双-（3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基分析2种叶片的体外抗氧化活性。结果表明：变异紫斑牡丹红色叶的颜色发生变化主要是芍药花素-3,5-二葡糖苷和天竺葵素-3,5-二葡糖苷含量上升与叶绿素含量下降的共同作用结果；变异紫斑牡丹红色叶中单体酚含量（绿原酸、芦丁、儿茶素、表二茶素、二氢杨梅酮、对香豆酸和二氢槲皮素）比紫斑牡丹绿色叶中的高；变异紫斑牡丹红色叶较紫斑牡丹的绿色叶相比有较好的抗氧化能力，具有潜在药用价值。
- Abstract:
- The leaves of Paeonia rockii and Paeonia rocki var.purpurea were used as experimental materials.Pigments in leaves of Paeonia rockii and Paeonia rocki var.purpurea were qualitatively identified and quantitatively analyzed using HPLC-Diode array detector(DAD)，UV sphotospectrometry and thin layer chromatography；1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trin-itrophenyl) hydrazyl(DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate(ABTS+?) were used to evaluate the in vitro antioxidant activity of the leaves.The results showed that the color-leaf variation of Paeonia rocki var. purpurea was major caused by the combined effect of the increased accumulation of peonidin-3，5-di-O-glucoside，pelargonidin-3，5-di-O-glucoside and decreased accumulation of chlorophyll.The red-leaf of Paeonia rocki var.purpurea had higher content on chlorogenic acid，rutin，epicaetchin，dihydromyricetin，p-coumaric acid and dihydroquercetin than green-leaf of Paeonia rockii；compared with green-leaf of Paeonia rockii，the red-leaf of Paeonia rocki var. purpurea exhibitted stronger antioxidant activity，which had potential medicinal value.
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备注/Memo
第一作者简介：唐豆豆（1988-），女，硕士研究生，现主要从事园林植物分子生物学等研究工作。E-mail：1747662070@qq.com.责任作者：李厚华（1973-），男，博士，副教授，现主要从事植物分子生物学等研究工作。E-mail：lihouhua73@163.com.基金项目：林业公益性行业科研重大专项资助项目（201404701）；西北农林科技大学基础科研业务费资助项目（2452015040）。