WEI Jianmei,QI Xiudong,YAN Fangjiao.Sugar and Starch Metabolism and the Key-enzyme Gene Expression in Postharvest ‘Gala’ Apple Fruit[J].Northern Horticulture,2015,39(19):126-131.[doi:10.11937/bfyy.201519031]
采后‘嘎拉’苹果果实糖和淀粉代谢及关键酶基因表达特性
- Title:
- Sugar and Starch Metabolism and the Key-enzyme Gene Expression in Postharvest ‘Gala’ Apple Fruit
- 文献标志码:
- A
- 摘要:
- 以‘嘎拉’苹果为试材，研究了采后果实后熟软化过程中可溶性糖和淀粉含量、相关酶活性及关键酶基因表达的变化，分析了低温、乙烯因子（1-MCP、乙烯利）处理对它们的影响，探讨了糖和淀粉代谢与采后‘嘎拉’果实软化的关系。结果表明：‘嘎拉’果实软化的初始阶段，淀粉降解对果实软化的影响最显著，并伴随淀粉酶（AM）活性和MdAM基因表达的快速上升，且淀粉含量、AM活性及它们与硬度的相关性均显著受到低温和乙烯因子的调节，表明淀粉降解与‘嘎拉’ 果实软化关系密切。糖代谢中，蔗糖、果糖和葡萄糖含量变化与果实软化表现显著的相关性，均受到低温和乙烯因子的调节，但仅有蔗糖磷酸合成酶（SPS）活性与果实硬度的变化显著相关。随着果实软化SPS活性和MdSPS基因的表达量不断增加，并显著受乙烯利的促进，受低温与1-MCP的抑制，其活性及其与硬度间的相关性也受到同样的影响，表明蔗糖代谢参与了‘嘎拉’果实的后熟软化，SPS在蔗糖代谢中起重要作用。
- Abstract:
- Taking ‘Gala’ apple fruits as the test materials，the changes in the contents of soluble sugars and starch were analyzed in order to study the relationship between carbohydrate metabolism and fruit softening，the activities of related enzymes，and the key-enzyme gene expression after being treated at low temperature and with 1-MCP and ethephone.The results showed that the starch contents decreased rapidly and it played the most significant roles at the early stage of storage，and accompanied by a rapid rise rate in AM activity and MdAM gene expression，and either the starch content and AM activity or their correlation with firmness were significantly regulated by ethylene factor and low temperature treatment，showing that starch degradation had close correlations with ‘Gala’ fruit softening.The changes of sucrose，glucose and fructose contents showed significant correlations with fruit softening，which significantly regulated by low temperature and ethylene factor treatment.However，only the SPS activity increased and had significantly correlation with firmness loss，accompanied with a fast rise rate in the expression of MdSPS gene.Also，these phenomena were significantly promoted by ethephon treatment and obviously inhibited by low temperature and 1-MCP treatments，and the correlation between SPS activity and fruit firmness were affected in the same way.So above results showed that SPS played an important role in ‘Gala’ fruit softening by involving in sucrose metabolism.
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备注/Memo
第一作者简介：魏建梅（1973-），女，河北昌黎人，博士，现主要从事果实品质发育与贮藏生理等研究工作。E-mail：xbndwjm@126.com.基金项目：河北省科技支撑计划资助项目(12226806)。