ZHANG Zhuli,JIANG Yanqin,YU Hong.Induction of Bud on Vaccinium bracteatum Thunb.[J].Northern Horticulture,2015,39(17):79-82.[doi:10.11937/bfyy.201517020]
乌饭树芽诱导体系的建立
- Title:
- Induction of Bud on Vaccinium bracteatum Thunb.
- 文献标志码:
- A
- 摘要:
- 以乌饭树单芽茎段和幼嫩叶片为试材，通过比较不同消毒方法、取材部位、茎段放置方式以及激素对乌饭树初代培养的作用，研究了不同因素对其初代培养中芽诱导效果的影响。结果表明：75%酒精 30 s+0.1%升汞 6 min消毒效果最佳，污染率为0，褐化率较低，萌芽率较高，展叶天数较早，且消毒后的外植体生长状况最佳；乌饭树单芽茎段较其叶片用作试验材料较好；放置方式以茎段横置于培养基中较佳；相较其它激素，ZT对乌饭树的诱导效果好，而各浓度的ZT中，以4 mg/L 效果最佳。
- Abstract:
- The tender leaves and single-bud stem segment of Vaccimium bracteatum Thunb.were used as the explants to study the effect of different factors on bud induction by comparing the function of different disinfection methods,material parts,the way of placement and hormone.The results showed that a combination way of 30 seconds with 75% alcohol and then 6 minutes with 0.1% mercuric chloride reached the best effect;the single-bud stem segment was more suitable than tender leaves as the explants；the single-bud stem segment transverse in the culture medium was better;the ZT had better induction effect than other hormones,and the concentration of 4 mg/L was the best.
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备注/Memo
第一作者简介：张祝丽（1985-），女，安徽安庆人，硕士，现主要从事蓝浆果组培等研究工作。E-mail：zhulizhang1107@163.com.基金项目：国家农业部公益性行业（农业）科研专项资助项目（201103037）；江苏省农业自主创新资助项目(CX（11）1011)。