CHENG Xiaojing,ZHOU Chuqi,ZHOU Liying,et al.RAPD Analysis on Three Cucurbita ficifotia[J].Northern Horticulture,2015,39(17):75-78.[doi:10.11937/bfyy.201517019]
三种黑籽南瓜RAPD分析
- Title:
- RAPD Analysis on Three Cucurbita ficifotia
- Keywords:
- Cucurbita ficifotia Bauche; genome DNA; RAPD; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以“绿皮”、“白皮”、“绿皮花纹”3种云南黑籽南瓜为试验材料，采用RAPD技术分析了3种类型黑籽南瓜间的亲缘关系。结果表明：从38种随机引物中筛选出20种引物，选取扩增清晰、重复性好的6种引物分析，共产生43条谱带，多态谱带39条，多态性位点比率为90.70％；“绿皮”、“白皮”和“绿皮花纹”之间的平均遗传距离是0.94，“白皮”和“绿皮花纹”间的平均遗传距离为0.40；“绿皮”与“白皮”及“绿皮花纹”间的亲缘关系较远，而“白皮”与“绿皮花纹”间的亲缘关系较近。
- Abstract:
- The genomic DNA of 3 Cucurbita ficifotia whose names were ‘Green peel’,‘White peel’ and ‘Green peel with White peel’ respectively were extracted by CTAB and were used to analysis the genetic relationships by RAPD molecular markers.RAPD was affected by many factors，so this experiment was for improved RAPD method.5 factors and 4 levels orthogonal design and screening of RAPD-PCR reaction system was used，the three kinds of black seed pumpkin relatedness were analyzed.The results showed that from 38 kinds of random primers selected 20 kinds of primers，6 kinds of the primers’analysis was clear amplification，and better repeatability.Total 43 bands were amplified，in which 39 bands were polymorphic．The percentage of polymorphic band was 90.70%．The cluster analysis results were indicated as follows：the average genetic distance among ‘Green peel’，‘White peel’ and ‘Green peel with white spot’ was 0.94.The genetic distance between ‘White peel’ and ‘green peel with white spot’ was 0.40.The results showed that the Cucurbita ficifotia varieties of relationships：‘Green peel’ and ‘White peel’，‘Green peel with white spot’ had far more distant relatives，and then‘White peel’ and ‘Green peel with white spot’ had close genetic relationship.
参考文献/References:
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备注/Memo
第一作者简介：成晓静（1990-），女，山西霍州人，硕士研究生，研究方向为蔬菜生物技术与遗传育种。E-mail：154313767@qq.com.责任作者：杨正安（1974-），男，云南昆明人，博士，副教授，研究方向为蔬菜生物技术与遗传育种。E-mail:454483788@qq.com.基金项目：云南省自然科学基金资助项目（2011FB049）；国家自然科学基金资助项目（31260481,31460516）。