WANG Weihua,CAO Yuan,MO Juntao,et al.Establishment of High Performance Liquid Chromatography Method forDetermination of Procyanidin From Musalais Wine in Xinjiang[J].Northern Horticulture,2015,39(16):126-130.[doi:10.11937/bfyy.201516030]
新疆慕萨莱思葡萄酒中原花青素含量测定的HPLC方法建立
- Title:
- Establishment of High Performance Liquid Chromatography Method forDetermination of Procyanidin From Musalais Wine in Xinjiang
- 文献标志码:
- A
- 摘要:
- 以矢车菊素、儿茶素、表儿茶素、表儿茶素没食子酸酯为标准品，采用岛津LC-20A高效液相色谱仪、二极管阵列检测器，建立了慕萨莱思酒中原花青素含量测定的HPLC方法。结果表明：优化后的色谱条件为色谱柱Inertsil DOS-3,5 μm 4.6 mm×250 mm色谱柱；流动相A为甲醇，流动相B为超纯水；流动相比例为流动相A为35%，流动相B为65%；流速1.0 mL/min；进样量10 μL；柱温38℃。应用所建立的HPLC方法对慕萨莱思酒进行分析，出峰效果良好；标准曲线正常，回收率为82%～93%。该方法操作简单、准确度高、测定时间短，可用于慕萨莱思酒中原花青素的测定。
- Abstract:
- Taking cyanidin,catechin,epicatechin and epicatechin-3-gallate as standards，a high performance liquid chromatograph (HPLC) method for determination of procyanidin content from Musalais was established using SHIMADZU LC-20A HPLC and diode array detector.The results showed that optimized chromatographic condition was as follow：column with Inertsil DOS-3，5 μm 4.6 mm×250 mm;mobile phase A was methanol，and mobile phase B was ultrapure water;proportion of mobile phase:mobile phase A was 35%，and mobile phase B was 65%;flow rate was 1 mL/min;sample volume was 10 μL;column temperature was 38℃.Using this HPLC method to detect Musalais, peak effect was well，standard curve was normal，and recoveries were 82% to 93%.This method had the advantages of simple operation,high accuracy，and short detection time.So it was successfully applied to determine procyanidins form Musalais.
参考文献/References:
[1]张新民.南疆一本就GO[M].北京:科学出版社,2011. [2]《走遍中国》编辑部.走遍中国:新疆[M].3版.北京:中国旅游出版社,2014. [3]朱丽霞,李红梅,郭东起,等.新疆慕萨莱思自然发酵过程中酵母菌表型多样性及优势菌分析[J].食品科学,2012,33(7):142-147. [4]朱丽霞,甄文,王丽玲,等.新疆慕萨莱思感官特性定量描述分析[J].食品科学,2013,34(1):38-44. [5]朱丽霞,侯旭杰,许倩.新疆慕萨莱思葡萄酒品质分析初探[J].酿酒科技,2008(6):68-71. [6]ZHU L,WANG L,SONG H,et al.Qualitative analysis of the main aroma compounds associated with traditional Musalais processing in Xinjiang,China [J].Journal of the Institute of Brewing,2012,118(2):236-242. [7]国植,徐莉.原花青素:具有广泛发展前景的植物药[J].国外医药(植物药分册),1996,11(1):196-204. [8]余晓琴,阚建全,赵国华,等.天然抗氧化剂原花青素保健功能[J].粮食与油脂,2003(4):40-42. [9]孙芸.葡萄籽原花青素的聚合度与功效关系的研究[D].无锡:江南大学,2004. [10]PEARSON D A,SCHMITZ H H,LAZARUS S A,et al.Inhibition of in vitro low-density lipoprotein oxidation by ligomeric procyanidins present in chocolate and cocoas[J].Methods Enzymol,2001,335:350-360. [11]LING Z Q,XIE B J,YANG E L.Isolation,characterization,and determination of antioxidative activity of oligomeric procyanidins from the seedpod of Nelumbo nucifera Gaertn [J].J Agric Food Chem,2005,53(7):2441-2445. [12]XIAO J S,XIE B J,CAO Y P,et al.Characterization of oligomeric procyanidins and identification of quercetin glucuronide from the lotus (Nelumbo nucifera Gaertn.)[J].J Agric Food Chem,2012,60(11):2825-2829. [13]RIOS L Y,BENNETT R N,LAZARUS S A,et al.Cocoa procyanidins are stable during gastric transit in humans[J].American J Clin Nutr,2002,76(5):1106-1110. [14]AUGER C,AL-AWWADI N,BORNET A,et al.Catechins and procyanidins in Mediterranean diets[J].Food Research International,2004(3):233-245. [15]崔介君,孙培龙,马新.原花青素的研究进展[J].食品科技,2003(2):292-295. [16]李春阳.葡萄籽中原花青素的提取纯化及其结构和功能研究[D].无锡:江南大学,2006. [17]凌智群,谢笔钧.莲房原花青素对氧自由基和脂质过氧化的作用[J].营养学报,2002,24(2):121-124. [18]常世敏,范蓓.红葡萄酒中原花青素的功能[J].中国食物与营养,2004(3):48-50. [19]赵文军,吴雪萍,王旭.葡萄籽低聚原花青素提取及性质分析[J].精细化工,2004,21(8):591-593. [20]吴朝霞.葡萄籽原花青素分离提纯、组分鉴定及抗氧化性研究[D].沈阳:沈阳农业大学,2005. [21]周素娟.葡萄籽提取物原花青素的研究概况及其在我国保健食品中的应用[J].中国食品卫生杂志,2007,19(3):284-286. [22]王伟华,韩占江.新疆慕萨莱思酒天然活性成分的代谢组学研究进展：以原花青素为例[J].食品安全质量检测学报,2013,4(6):1808-1812. [23]王燕,王方,张岱,等.葡萄酒有机酸的HPLC分析中色谱条件的优化[J].中外葡萄与葡萄酒,2010(9):34-37. [24]王夏青,於洪建,赵余庆.干红葡萄酒中儿茶素、表儿茶素、白藜芦醇和原花青素的测定[J].中草药,2009,40(5):745-747. [25]李春阳,许时婴,王璋.葡萄籽原花青素结构单元的红外光谱分析[J].食品与生物技术学报,2005(4):47-51.
相似文献/References:
[1]李琳玲,程 华,吴聪华,等.SPE-HPLC法同时分离测定板栗花芽中四种内源激素的研究[J].北方园艺,2014,38(01):22.
LI Lin-ling,CHENG Hua,WU Cong-hua,et al.Simultaneous Determination of 4 Endogenous Hormones in Chinese Chestnut Floral Bud by Solid Phase Extraction-High Performance Liquid Chromatography[J].Northern Horticulture,2014,38(16):22.
[2]朱金霞,郑国保,孔德杰,等.甘草中甘草苷、甘草酸及总黄酮的测定及其含量相关性研究[J].北方园艺,2014,38(03):149.
ZHU Jin-xia,ZHENG Guo-bao,KONG De-jie,et al.Study on the Determination of Liquiritin,Glycyrrhizic Acid and Total Flavonoids in Licoric and Their Relationships[J].Northern Horticulture,2014,38(16):149.
[3]步佳佳,胡玉林,魏长斌,等.利用HPLC法对香蕉根中四种内源激素测定方法的优化[J].北方园艺,2012,36(08):30.
BU Jia-jia,HU Yu-lin,WEI Chang-bin,et al.The Optimum of HPLC Conditions of Four Kinds of Endogenous Hormones in the Roots of Banana[J].Northern Horticulture,2012,36(16):30.
[4]刘倩,康如龙,苏小建.高效液相色谱-蒸发光散射检测法测定果汁中单糖的含量[J].北方园艺,2012,36(21):36.
LIU Qian,KANG Ru-long,SU Xiao-jian.Research on Free Sugars of Juice By HPLC-ELSD[J].Northern Horticulture,2012,36(16):36.
[5]王 鹏,马玲玲,陈 雨,等.盐胁迫对油葵种子萌发及内源激素含量的影响[J].北方园艺,2015,39(03):12.[doi:10.11937/bfyy.201503004]
WANG Peng,MA Ling-ling,CHEN Yu,et al.Effect of Salt Stress on Seed Germination and Endogenous Hormones of Oil Sunflower[J].Northern Horticulture,2015,39(16):12.[doi:10.11937/bfyy.201503004]
[6]曹慧,徐文娟,陈志章,等.苹果多酚物质组分的高效液相色谱分析[J].北方园艺,2016,40(07):1.[doi:10.11937/bfyy.201607001]
CAO Hui,XU Wenjuan,CHEN Zhizhang,et al.Analysis of the Composition of Apple Phenolics by High Performance Liquid Chromatography (HPLC)[J].Northern Horticulture,2016,40(16):1.[doi:10.11937/bfyy.201607001]
[7]李媛媛,王冰林,张军,等.不同萝卜肉质根中可溶性糖组分及含量分析[J].北方园艺,2017,41(04):4.[doi:10.11937/bfyy.201704002]
LI Yuanyuan,WANG Binglin,ZHANG Jun,et al.Component and Content Analysis of Soluble Sugar in Various Radish Cultivars[J].Northern Horticulture,2017,41(16):4.[doi:10.11937/bfyy.201704002]
[8]姚小桐,张润芝,许宁,等.不同品种芸薹属蔬菜中硫代葡萄糖苷含量的比较分析[J].北方园艺,2018,42(21):30.[doi:10.11937/bfyy.20180890]
YAO Xiaotong,ZHANG Runzhi,XU Ning,et al.Analysis of Glucosinolate Content in Different Brassica Vegetables[J].Northern Horticulture,2018,42(16):30.[doi:10.11937/bfyy.20180890]
[9]徐世娟,卢海啸,黄华希,等.引种紫锥菊中松果菊苷含量的分析测定[J].北方园艺,2019,43(09):119.[doi:10.11937/bfyy.20183502]
XU Shijuan,LU Haixiao,HUANG Huaxi,et al.Determination of Echinacoside in Introducing Plant of Echinacea purpurea[J].Northern Horticulture,2019,43(16):119.[doi:10.11937/bfyy.20183502]
[10]廖霞,詹永,杨勇,等.不同年份蕲艾酚类化合物及抗氧化活性[J].北方园艺,2023,(05):110.[doi:10.11937/bfyy.20222867]
LIAO Xia,ZHAN Yong,YANG Yong,et al.Polyphenols and Antioxidant Activity of Artemisia argyi (Qichun) in Different Storage Years[J].Northern Horticulture,2023,(16):110.[doi:10.11937/bfyy.20222867]
备注/Memo
第一作者简介：王伟华（1977-），女，吉林双阳人，博士，副教授，现主要从事食品质量安全等研究工作。E-mail：wangweihua6688@163.com. 基金项目：国家自然科学基金资助项目（31360409，31471667）；新疆生产建设兵团“兵团英才”选拔培养工程（第一周期第三层次培养人选）资助项目。