LIU Duling,ZHANG Boyong,PENG Shaobing,et al.Effect of Different Storage Temperature and Time on Pollen Viability of Walnut Cultivars[J].Northern Horticulture,2015,39(16):7-10.[doi:10.11937/bfyy.201516002]
贮藏温度及时间对核桃品种花粉生活力的影响
- Title:
- Effect of Different Storage Temperature and Time on Pollen Viability of Walnut Cultivars
- Keywords:
- walnut; pollen vitality; storage conditions; storage method
- 文献标志码:
- A
- 摘要:
- 以“香玲”、“西林3号”、“西洛2号”、“强特勒”和“哈特勒”5个核桃品种的花粉为试材，用TTC染色法和离体培养法测定了不同贮藏温度及时间下花粉的生活力。结果表明：5个核桃品种花粉在室温（20℃）下贮藏生活力最低，在3℃和-20℃下贮藏生活力较高；随贮藏时间延长花粉生活力显著下降。用TTC染色法测得的花粉生活力显著高于离体培养法，2种测定方法之间具有极显著的相关性。“香玲”、“西林3号”、“西洛2号”和“强特勒”花粉适于在3℃下贮藏，“哈特勒”花粉适于在-20℃下贮藏，贮藏时间为6～9 d。
- Abstract:
- Pollen vitality of 5 walnut cultivars ‘Xiangling’，‘Xilin 3’，‘Xiluo 2’，‘Chandler’ and ‘Hartley’ were determined by TTC staining and in vitro culture method under different storage temperatures and time．The results showed that，the pollen viability of 5 walnut cultivars were the lowest at room temperature (20℃) while higher at 3℃ and -20℃；with the extension of storage time，the pollen viability decreased significantly and TTC staining was significantly higher than in vitro culture method.Two methods for determining had a significant correlation.The suitable condition was at 3℃ for ‘Xiangling’，‘Xilin 3’，‘Xiluo 2’，‘Chandler’ pollen storage and at -20℃ for ‘Hartley’，the storage time was 6-9 days.
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备注/Memo
第一作者简介：刘杜玲（1964-），女，陕西富平人，硕士，副教授，现主要从事经济林栽培的理论与技术等研究工作。E-mail：liudl606@126.com.责任作者：吕平会（1959-），男，陕西周至人，本科，副研究员，现主要从事经济林栽培及育种等研究工作。E-mail：lvpinghui@126.com.基金项目：陕西省农业攻关资助项目(2014K01-10-01)；西北农林科技大学基本科研业务费专项资助项目（QN2013080）。