PAN Duofeng,SHEN Zhongbao,WANG Jianli,et al.Effect of Alkaloid-saline Stress on the Seed Germination and Seedling Growth of Trifolium repens[J].Northern Horticulture,2015,39(14):67-70.[doi:10.11937/bfyy.201514019]
碱性盐胁迫对白三叶种子萌发及幼苗生长的影响
- Title:
- Effect of Alkaloid-saline Stress on the Seed Germination and Seedling Growth of Trifolium repens
- 文献标志码:
- A
- 摘要:
- 以白三叶新品系‘Z005’为试材,研究了不同浓度(0、20、40、80、120、160、200 mmol/L)碱性盐溶液(NaCl、Na2SO4、NaHCO3和Na2CO3)对种子萌发及幼苗生长的影响。结果表明:低浓度胁迫(≤20 mmol/L)提高白三叶种子的萌发能力、促进幼苗生长,而高浓度(>40 mmol/L)则表现为抑制作用。当溶液浓度大于80 mmol/L,发芽率、发芽势、发芽指数、活力指数、苗高和根长均极显著低于对照(P<0.01)。白三叶种子萌发适宜的碱性盐溶液浓度应小于94.50 mmol/L。碱性盐溶液对白三叶根生长的抑制作用强于对苗的抑制作用。
- Abstract:
- Taking Trifolium repens new strain (‘Z005’) as material,the effect of different concentration (0,20,40,80,120,160,200 mmol/L)saline-alkaloid (NaCl,Na2SO4,NaHCO3,Na2CO3)stress on the seed germination and seedling growth of Trifolium repens new strain (‘Z005’) were studied.The results showed that low concentration of saline-alkaloid stress(concentration was≤20 mmol/L) could improve seed germination ability and seedling growth of Trifolium repens,contrastly,high concentration decreased seed germination and seedling growth.When the concentration was exceed 80 mmol/L,germination rate,germination potential,germination index,vigor index,root length and shoot height were significantly lower compared with control (P<0.01). Seed of Trifolium repens could germinate under the condition that the concentration of saline-alkaloid below 94.50 mmol/L.And saline-alkaloid stress had a higher inhibition to root length than shoot height.
参考文献/References:
[1]TANJI K K.Agricultural salinity assessment and management[M].New York:American Society of Civil Engineers,1990:1-112. [2]徐恒刚.中国盐生植被及盐渍化生态治理[M].北京:中国农业科学技术出版社,2004:1. [3]俞仁培,陈德明.我国盐渍土资源及其开发利用[J].土壤通报,1999,30(4):158-159. [4]宋长春,何岩,邓伟.松嫩平原盐渍土壤生态地球化学[M].北京:科学出版社,2003:64-90. [5]刘兴土.松嫩平原退化土地整治与农业发展[M].北京:科学出版社,2001:60-135. [6]李取生,裘善文,邓伟.松嫩平原土地次生盐渍化研究[J].地理科学,1998,18(3):268-272. [7]申忠宝,潘多锋,王建丽,等.混合盐碱胁迫对5种禾草种子萌发及幼苗生长的影响[J].草地学报,2012,20(5):914-920. [8]尹红娟.虎尾草对盐碱混合胁迫的生理响应特点[D].长春:东北师范大学,2008:7. [9]THOMPSON D I,EDWARDS T J,van STADEN J.A novel dual-phase culture medium promotes germination and seedling establishment from immature embryosin South African Disa (Orchidaceae) species[J].Plant Growth Regul,2007,53:163-171. [10]刘杰,张美丽,张义,等.人工模拟盐、碱环境对向日葵种子萌发及幼苗生长的影响[J].作物学报,2008,34(10):1818-1825. [11]KATERJI N,van HOORN J W,HAMDY A,et al.Salt tolerance classification of crops according to soil salinity and to water stress day index[J].Agric WaterManag,2000,43:99-109. [12]KATERJI N,van HOORN J W,HAMDY A,et al.Salt tolerance of crops accordingto three classification methods and examination of some hypothesis about salt tolerance[J].Agric Water Manag,2001,47:1-8. [13]王建丽,申忠宝,高洪文,等.21份白三叶种质资源的农艺性状比较研究[J].黑龙江畜牧兽医,2012(10):73-75. [14]赵桂琴,王锁民,任继周.白三叶转基因及其生态适应性研究进展[J].生态学报,2004,24(3):592-598. [15]张鹤山,陈明新,田宏,等.高温胁迫下白三叶种子萌发特性及耐热性研究[J].种子,2010,29(8):1-5. [16]贾文庆,刘会超,何莉.盐分胁迫下白三叶种子的发芽特性研究[J].草业科学,2007,24(9):55-57. [17]吴海涛,刘芳,赵丹,等.盐胁迫对三种白三叶种子萌发的影响[J].四川畜牧兽医,2008(8):35-38. [18]殷秀杰,燕昌江,李凤兰,等.混合盐碱胁迫对白三叶种子萌发的影响[J].东北农业大学学报,2009,40(12):58-61. [19]国际种子检验协会(ISTA).国际种子检验规程[M].北京:中国农业出版社,1999:4-9. [20]韩萌,张月学,潘多锋,等.混合盐碱胁迫对3种无芒雀麦种子萌发及幼苗生长的影响[J].黑龙江农业科学,2012(7):119-122. [21]XU X Y,FAN R,ZHANG R,et al.Proteomic analysis of seed germination under salt stress in soybeans[J].Journal of Zhejiang University Science B:Biomedicine and Biotechnology,2011,12(7):507-517. [22]CHERIAN S,REDDY M P,PANDYA J B.Studies on salt tolerance in Avicennia marina(Forstk.)Vierh:effect of NaCl salinity on growth,ion accumulation and enzyme activity[J].Plant Physiol,1999,4:266-270. [23]卢艳敏,苏长青,李会芬.不同盐胁迫对白三叶种子萌发及幼苗生长的影响[J].草业学报,2013,22(4):123-129. [24]RUBIO A E,CASTILLO J M,LUQUE C J,et al.Influence of salinity on germinationand seeds viability of two primary colonizers of Mediterranean salt pans[J].J Arid Environ,2003,53:145-154. [25]杨春武,贾娜尔·阿汗,石德成,等.复杂盐碱条件对星星草种子萌发的影响[J].草业学报,2006,15(5):45-51. [26]MOHAMMAD M.Tomato root and shoot responses to salt stress under different levels of phosphorus nutrition[J].Plant Nutr,1998,21:1667-1680. [27]SHI D,WANG D.Effect of various salt-alkaline mixed stress conditions on Leymus chinensis (Trin.)[J].Plant Soil,2005,271:15-26.
相似文献/References:
[1]刘 海,齐 孝 辉,韩 雪.干旱胁迫及复水对四个白三叶品种叶片保护酶活性的影响[J].北方园艺,2010,34(05):0.[doi:10.201005037]
LIU Hai -yan,QI Xiao -hui,HAN Xue.Effect of Drought and Water Recovers on Protective Enzymes Activities of 4 White Clovers[J].Northern Horticulture,2010,34(14):0.[doi:10.201005037]
[2]金 忠 民,沙 伟,张 艳 馥,等.白三叶种子愈伤组织诱导及再生体系的建立[J].北方园艺,2010,34(12):0.[doi:10.11937/bfyy.2010012054]
JIN Zhong-min,SHA Wei,ZHANG Yan -fu,et al.Establishmengt of Regeneration System and Callus Inducement in Seed of Trifolium repens Linn[J].Northern Horticulture,2010,34(14):0.[doi:10.11937/bfyy.2010012054]
[3]韦新东,黄一格,王颖.镉、铅胁迫对白三叶种子萌发及幼苗生长的影响[J].北方园艺,2016,40(02):71.[doi:10.11937/bfyy.201602019]
WEI Xindong,HUANG Yige,WANG Ying.Effect of Heavy Metal Cadmium and Lead Stress on Seed Germination and Seedlings Growth of the Trifolium repens[J].Northern Horticulture,2016,40(14):71.[doi:10.11937/bfyy.201602019]
[4]李 志 亮,等.白 三 叶 转 基 因 研 究 进 展[J].北方园艺,2009,33(07):0.[doi:10.11937/bfyy.200907053]
LI Zhi - liang,XING Hao - chun,et al.Advancement of the Research on T ransgenic White Clover[J].Northern Horticulture,2009,33(14):0.[doi:10.11937/bfyy.200907053]
[5]王晶晶,李娜,张身嗣.覆盖作物白三叶对蓝莓园杂草的生物防除效果[J].北方园艺,2017,41(03):138.[doi:10.11937/bfyy.201703031]
WANG Jingjing,LI Na,ZHANG Shensi.Biological Control of Cover Crops White Clover Against Weeds in Blueberry Orchard[J].Northern Horticulture,2017,41(14):138.[doi:10.11937/bfyy.201703031]
[6]周莹,王明玖,陈国清,等.高加索三叶草与白三叶杂交 F1代株系的ISSR分析[J].北方园艺,2017,41(12):103.[doi:10.11937/bfyy.201712025]
ZHOU Ying,WANG Mingjiu,CHEN Guoqing,et al.ISSR Analysis for Hybrid F1 of Trifolium ambiguum Bieb.and T.repens L.[J].Northern Horticulture,2017,41(14):103.[doi:10.11937/bfyy.201712025]
备注/Memo
第一作者简介:潘多锋(1980-),男,宁夏青铜峡人,硕士,助理研究员,现主要从事牧草育种与草地生态等研究工作。E-mail:panduofeng2000@163.com.责任作者:申忠宝(1973-),男,黑龙江讷河人,硕士,研究员,现主要从事牧草育种和栽培等研究工作。E-mail:shzhbao2@126.com.基金项目:黑龙江省农业科技创新工程院级科研资助项目(2012ZD001)。