SUN Jingbo,YU Jing,AN Liping,et al.Extraction and Determination of Total Flavonoids From Cordyceps militaris Clutrued by Different Cultural Medium[J].Northern Horticulture,2015,39(13):137-139.[doi:10.11937/bfyy.201513039]
不同来源北冬虫夏草中总黄酮的提取及含量测定
- Title:
- Extraction and Determination of Total Flavonoids From Cordyceps militaris Clutrued by Different Cultural Medium
- 文章编号:
- 1001-0009（2015）13-0137-03
- 分类号:
- S 6;S 567.3+5
- 文献标志码:
- B
- 摘要:
- 以不同来源北冬虫夏草为试材，通过正交实验研究总黄酮的提取工艺，以紫外吸收峰的峰形、位置及强度为标准选定显色试剂及测定波长，确定北冬虫夏草中总黄酮的提取工艺及含量测定方法。结果表明：用40倍量的70%乙醇于90℃水浴中单次回流提取4 h，总黄酮提取率最高；以芦丁为对照品，0.1 mol/L的AlCl3为显色剂，265 nm为检测波长，测定总黄酮的含量方法最佳；以大米为培养基的北冬虫夏草（米虫草），蚕蛹培养（蛹虫草）的北冬虫夏草子实体、全草及虫体中总黄酮含量分别为3.700 8、4.039 8、3.219 2、2.502 4 mg/g。
- Abstract:
- Taking Cordyceps militaris from different sources as test materials，the extraction technology of total flavonoids in C.militaris was optimized by orthogonal test，the chromogenic agent and the determine wavelength selection depended on the shapes，positions and intensity of UV absorbance bands.The results showed that total flavonoids content in C.militaris was the highest when refluxed extracted with 40 times of 70% alcohol for 4 hours (90℃)，and then determined by UV at 265 nm after being treated with 0.1 mol/L AlCl3.The total flavonoids contents in C.militaris growing on rice medium，and in fruit bodies，whole grass，pupa body of C.militaris growing on silkworm pupa were 3.700 8 mg/g，4.039 8 mg/g，3.219 2 mg/g，2.502 4 mg/g，respectively.
参考文献/References:
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备注/Memo
第一作者简介：孙晶波（1978-），女，博士，副教授，研究方向为药用植物活性成分及其功用。E-mail：sjb781219@163.com.责任作者：杜培革（1964-），女，博士，教授，研究方向为微生物与生化药学。E-mail：2207520094@qq.com.基金项目：吉林市科技计划具有产业化前景的科技创新攻关专项资助项目（201464045），吉林市科技支撑计划农业科技创新专项资助项目（2013222010）。