SHAO Qian-shun,GAO Lei,NAN Xiong-xiong,et al.Analysis of Genetic Diversity and Construction of Fingerprint of Lycium bararum L.Using SSR Technology[J].Northern Horticulture,2015,39(12):91-95.[doi:10.11937/bfyy.201512024]
利用SSR技术对十七份枸杞材料进行遗传多样性分析及标准指纹图谱构建
- Title:
- Analysis of Genetic Diversity and Construction of Fingerprint of Lycium bararum L.Using SSR Technology
- 文章编号:
- 1001-0009（2015）12-0091-05
- Keywords:
- Lycium bararum L.; SSR; genetic diversity; fingerprint map
- 分类号:
- S 567.1+9
- 文献标志码:
- A
- 摘要:
- 以17个枸杞资源为试材，采用简单重复序列（Simple Sequence Repeat，SSR）技术，研究宁夏枸杞系列品种和其它枸杞种/品种及野生类型的遗传多样性和指纹图谱。结果表明：以15对SSR引物对17份枸杞种质扩增获得109个位点，其中91个多态性位点，多态性比例83.4%；17个种质平均多样性指数H为0.1943，Shannon信息指数I为0.3108；10个宁夏枸杞栽培品种的Nei’s多样性指数H为0.0962，Shannon信息指数I为0.1537；7个野生种质的Nei’ s多样性指数H为0.2004，Shannon信息指数I为0.3130，说明非栽培枸杞的多样性比栽培品种的多样性更丰富。以其中4对引物构建了17个枸杞种质的DNA指纹图谱。该研究结果为枸杞品种鉴定、遗传图谱构建等奠定了技术基础。
- Abstract:
- Genetic diversity of Ningxia wolfberry series varieties and other Chinese wolfberry species/varieties and wild type were analyzed using Simple Repeat sequences (SSR) technology with 17 wolfberry germplasm，also their fingerprint map was constructed for the first time.The results showed that 17 Chinese wolfberry germplasm was checked with 15 pairs of SSR primers，and 91 polymorphic loci of 109 loci were obtained，the proportion of polymorphism was 83.4%；the average diversity index H of 17 germplasm was 0.1943，the Shannon information index I was 0.3108；the Nei ‘ s diversity index H of 10 ningxia wolfberry cultivars was 0.0962，the Shannon information index I was 0.1537；the Nei ’s diversity index H of 7 wild germplasm was 0.2004，Shannon information index I was 0.3130.It’s proved that the diversity of the not cultivated wolfberry was richer than that of cultivated varieties.The fingerprint map of 17 Chinese wolfberry germplasm was constructed with four pairs of primers.Our study laid a solid foundation for the identification of Chinese wolfberry species and the construction of genetic map.
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备注/Memo
第一作者简介： 邵千顺（1986-），硕士，助理研究员，研究方向为分子遗传育种。Email:shaoqianshun@126.com.基金项目： 农业科技成果转化资金资助项目（2012GB300478）。