LIU Shun-zhi,ZHOU Hao-bin,LIN Run-yi,et al.Optimizating of Aerial Root Chromosome Preparation Technology and Karyotype Analysis of Red Pitaya( Hylocereus polyrhizus)[J].Northern Horticulture,2015,39(12):71-76.[doi:10.11937/bfyy.201512020]
红肉火龙果气生根染色体制片优化及核型分析
- Title:
- Optimizating of Aerial Root Chromosome Preparation Technology and Karyotype Analysis of Red Pitaya( Hylocereus polyrhizus)
- 文章编号:
- 1001-0009（2015）12-0071-06
- Keywords:
- red pitaya; aerial root; chromosome; preparation technique; karyotype analysis
- 分类号:
- S 667.9
- 文献标志码:
- A
- 摘要:
- 以红肉火龙果的气生根为试材，研究气生根长度、采样时间、预处理方法、解离时间和染色方法等对红肉火龙果气生根根尖细胞染色体制片效果的影响，以优化的制片技术制片并进行核型分析。结果表明：10:30时段取长度5 mm以下的幼嫩气生根，用0.1%秋水仙素常温预处理2 h，应用双低渗法使染色体分散，常温下1 mol/L HCl解离50 min或60℃下解离20 min，20%改良苯酚-品红染色液染色20 min，可以得到最佳的制片效果，用中性树脂制成永久装片。染色体核型分析表明，红肉火龙果的染色体数目为11对，最长染色体/最短染色体为1.63，核型公式为2n＝2x＝22＝22m，核型不对称系数为54.29%，核型属1A类型。
- Abstract:
- Taking aerial root tips of red pitaya (Hylocereus polyrhizus) as material for chromosome preparation，the effect of root length，sampling time，permanent，dissociation time and staining on cytological observation were analyzed. The results showed that the optimum chromosome preparation was achieved with collecting root tips at 10:30 AM，root length within 5 mm，pretreated for 2 hours in 0.1% colchicine at room temperature，spreading chromosomes by double hypotonic treatment，dissociation with 1 mol/L HCl for 50 minutes at room temperature or 20 minutes at 60℃，and stained with 20% modified carbol fuchsin dyeing.The permanent slides could be made by the neutral resin.The karyotype analysis indicated that red pitaya is diploid(2n＝2x＝22)，and the ratio of the longest chromosome to the shortest one was 1.63，so that the karyotype formula of red pitaya was 2n＝22m，belonging to 1A karyotype.The karyotype asymmetrical coefficient was 54.29%.
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备注/Memo
第一作者简介： 刘顺枝(1968-), 女,硕士, 副教授, 现主要从事果树栽培生理与分子生物学等研究工作。E-mail:shunzhiliu@163.com. ?责任作者： 胡位荣(1966-), 男, 博士, 教授, 现主要从事果蔬采后生物学等研究工作。E-mail:weironghu@163.com.基金项目： 广州市科技计划资助项目(2014J4100143)；广州市属高校科研计划资助项目(2012A047);广东省高等院校学科建设专项资助项目（粤财教(2008)342）。