ZHANG Yue,WANG Jian-li,WANG Xiu-feng,et al.Establishment of Wild Lilium Tissue Culture System[J].Northern Horticulture,2015,39(11):95-97.[doi:10.11937/bfyy.201511023]
野生细叶百合组织培养体系的建立
- Title:
- Establishment of Wild Lilium Tissue Culture System
- 文章编号:
- 1001-0009(2015）11-0095-03
- Keywords:
- Lilium pumilum; organogenesis; somatic embryogenesis
- 分类号:
- S682.2+65
- 文献标志码:
- A
- 摘要:
- 以细叶百合为试材，采用组织培养的方法，研究细叶百合不同部位、不同激素配比及不同发生途径对获得细叶百合再生植株的影响。结果表明：以鳞茎为外植体，通过器官发生途径获得再生植株，其最佳分化及增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L；以花器官、幼嫩叶片为外植体通过体细胞发生途径获得再生植株，其愈伤组织诱导培养基为MS+2,4-D 2.0 mg/L+NAA 1.5 mg/L，不定芽诱导培养基为MS+6-BA 1.0 mg/L+IAA 0.1 mg/L，最佳增殖培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,生根培养基为1/2MS+NAA 0.1 mg/L。
- Abstract:
- Taking Lilium pumilum as experimental material,using tissue culture method,effects of different parts of Lilium pumilum, different hormone combinations and different ways of plant regeneration on Lilium pumilum were studied.The results showed that with the bulb as explants,the organ regeneration plants were obtained,the best medium for differentiation and proliferation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L；in flower organ,the young leaves as explants via somatic embryo genesis regeneration plants were obtained,and the callus induction medium was MS+2,4-D 2.0 mg/L+NAA 1.5 mg/L，adventitious bud induction medium was MS+6-BA 1.0 mg/L+IAA 0.1 mg/L，the optimal proliferation medium was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.Rooting culture medium was 1/2MS+NAA 0.1 mg/L.
参考文献/References:
[1] 葛蓓孛，杨青杰，吴萍，等．细叶百合组织培养植株再生[J]．东北林业大学学报,２０１０,３８(５):５４-５９.[2] 吴若著,阮少宁.百合胚状体形成途径及植株的再生研究[J].福建林学院学报,2003,23(4):322-325.[3] 万勇,毛凌华,黄永萍,等.万载百合组织培养快速繁殖的研究[J].江西农业学报,2000,12(4):26-29.[4] 唐东芹,黄丹枫,唐克轩,等.东方百合鳞片的组织培养[J].植物生理学通讯,2003,39(5):450-452.[5] 王元忠,沙本才.南川百合的组织培养和快速繁殖[J].亚热带植物科学,2008,37(3):69-70.[6] 张艺萍,屈云慧,吴学尉,等.大理百合的组织培养和快速繁殖[J].北方园艺,2007(8):189-190.[7] 周祖富,艾素云,杨美纯.火百合花丝组织培养器官形成的细胞组织学研究[J].亚热带植物科学,2006,41(3):1-15.[8] 刘菊华,金志强,徐碧玉,等.龙牙百合的植株再生与遗传转化[J].分子植物育种,2003，4(1):465-474.
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备注/Memo
第一作者简介：张悦(1983-)，女,硕士，助理研究员，现主要从事园艺作物组织培养等研究工作。E-mail:55757703@qq.com.基金项目：吉林省科技厅资助项目(20120262）。