[1]王 崇,程玉祥.AtGDPD-Like 6和AtGDPD-Like 7基因启动子表达特性及AtGDPD-Like 6蛋白定位[J].北方园艺,2015,39(11):90-94.[doi:10.11937/bfyy.201511022]
　WANG Chong,CHENG Yu-xiang.The Expression Patterns of Arabidopsis thaliana GDPDL6 and GDPDL7 and the Subcellular Localization of GDPDL6[J].Northern Horticulture,2015,39(11):90-94.[doi:10.11937/bfyy.201511022]
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AtGDPD-Like 6和AtGDPD-Like 7基因启动子表达特性及AtGDPD-Like 6蛋白定位

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年11 页码: 90-94 栏目: 出版日期: 2015-06-15
Title:
The Expression Patterns of Arabidopsis thaliana GDPDL6 and GDPDL7 and the Subcellular Localization of GDPDL6
文章编号:
1001-0009(2015)11-0090-05
作者:
王 崇; 程玉祥
东北林业大学 林木遗传育种国家重点实验室，黑龙江 哈尔滨 150040
Author(s):
WANG Chong; CHENG Yu-xiang
State Key Laboratory of Tree Genetics and Breeding，Northeast Forestry University，Harbin,Heilongjiang 150040
关键词:
拟南芥; GDPD-Like; 表达模式; 蛋白定位
Keywords:
Arabidopsis thaliana; GDPD-Like; expression pattern; subcellular localization
分类号:
Q 945
DOI:
10.11937/bfyy.201511022
文献标志码:
A
摘要:
以野生型(Col-0）拟南芥为试材，采用RT-PCR法，研究AtGDPDL6和AtGDPDL7基因组织表达特性。以ProAtGDPDL6::GUS和ProAtGDPDL7::GUS转基因拟南芥为试材，采用GUS组织化学染色法，研究AtGDPDL6和AtGDPDL7基因启动子表达模式，并以Pro35S::AtGDPDL6-GFP转基因拟南芥为试材，采用激光共聚焦显微镜进行荧光观察，研究AtGDPDL6蛋白的细胞内定位。结果表明：AtGDPDL6基因在拟南芥花组织中特异表达；AtGDPDL7基因在花和角果中特异表达。AtGDPDL6和AtGDPDL7基因特异表达在拟南芥成熟的花粉囊和柱头中。AtGDPDL6蛋白定位于细胞膜和细胞壁区域。
Abstract:
Using wild type (Col-0) Arabidopsis thaliana as plant materials，the expression pattern of AtGDPDL6 and AtGDPDL7 was studied by RT-PCR.Using the ProAtGDPDL6::GUS and ProAtGDPDL7::GUS transgenic plants as plant materials，the expression pattern of AtGDPDL6 and AtGDPDL7 promoter were studied by GUS activity staining.Using the Pro35S::AtGDPDL6-GFP transgenic plants as plant materials，the subcellular localization of GDPDL6 was studied by fluorescence microscopy.The results showed that AtGDPDL6 was specifically expressed in flowers，and AtGDPDL7 was expressed in flowers and siliques;ProAtGDPDL6::GUS and ProAtGDPD7::GUS were mainly expressed in mature pollen sacs and stigma;the AtGDPDL6 was localized to the plasma membrane or the cell wall.

参考文献/References:

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备注/Memo

第一作者简介：王崇(1989-），男，黑龙江哈尔滨人，硕士研究生，研究方向为林木遗传育种。E-mail:nefuwangchong@163.com.责任作者：程玉祥(1972-），男，教授，现主要从事树木分子遗传与育种等研究工作。E-mail:chengyuxiang@nefu.edu.cn.基金项目：国家“863”计划课题资助项目(2013AA102702）。

更新日期/Last Update: 2015-08-06