GAO Kun-mei,XING Zhi-wei,ZHENG Su-yue,et al.Selection and Identification of Hybrid Strain of Pleurotus eryngii var. tuoliensis[J].Northern Horticulture,2015,39(09):121-123.[doi:10.11937/bfyy.201509034]
白灵菇杂交子的筛选及鉴定
- Title:
- Selection and Identification of Hybrid Strain of Pleurotus eryngii var. tuoliensis
- 文章编号:
- 1001-0009（2015）09-0121-03
- Keywords:
- Pleurotus eryngii var. tuoliensis; hybrid strain; antagonistic test; isozyme analysis; primordium formation
- 分类号:
- S 567.3+ 9
- 文献标志码:
- A
- 摘要:
- 以白灵菇2个品种“中农1号”和“中农翅鲍”为亲本，采用镜检、拮抗、酯酶同工酶及原基诱导等方法，研究了150对杂交组合菌株特性。结果表明：150对杂交组合菌株中，99个菌株生长较快，有锁状联合，75个与2个亲本有明显拮抗线，且具与2个亲本菌株的典型酯酶同工酶条带，确定是杂交菌株，其中50个杂交子具有出菇能力。
- Abstract:
- Taking two different Pleurotus eryngii var. tuoliensis strains as parents (‘Zhongnong No.1’ and‘Zhongnong Chibao’),the proterties of 150 cross combinations were studied by microscopy，antagonism，esterase isozyme and primordium induction.The results showed that 99 of 150 hybrid strains were with clamp-connection，75 strains had obvious antagonism with two parents and had new enzyme bands and complementary enzyme bands，50 strains had primordium formation in PDA plate.
参考文献/References:
[1]王贺祥，刘庆洪.食用菌栽培学[M].北京：中国农业大学出版社，2014.
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备注/Memo
第一作者简介：高坤梅（1981-），女，硕士，现主要从事食用菌等研究工作。E-mail：gaokunmei@126.com.