[1]张彦妮,刘奕佳,李博.干旱胁迫及复水对大花飞燕草幼苗生理特性的影响[J].北方园艺,2015,39(09):58-62.[doi:10.11937/bfyy.201509018]
　ZHANG Yan-ni,LIU Yi-jia,LI Bo.Effect of Drought Stress and Rewatering on Physiological Characteristics of Delphinium grandiflorum Seedling[J].Northern Horticulture,2015,39(09):58-62.[doi:10.11937/bfyy.201509018]
点击复制

干旱胁迫及复水对大花飞燕草幼苗生理特性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年09 页码: 58-62 栏目: 出版日期: 2015-05-15
Title:
Effect of Drought Stress and Rewatering on Physiological Characteristics of Delphinium grandiflorum Seedling
文章编号:
1001-0009（2015）09-0058-05
作者:
张彦妮; 刘奕佳; 李博
（东北林业大学 园林学院，黑龙江 哈尔滨 150040）
Author(s):
ZHANG Yan-ni; LIU Yi-jia; LI Bo
（College of Landscape Architecture，Northeast Forestry University，Harbin，Heilongjiang 150040）
关键词:
大花飞燕草; 干旱胁迫; 生理特性
Keywords:
Delphinium grandiflorum; drought stress; physiological property
分类号:
S 681.9
DOI:
10.11937/bfyy.201509018
文献标志码:
A
摘要:
以大花飞燕草幼苗为试材，研究了干旱胁迫及复水条件对大花飞燕草幼苗膜脂过氧化和保护酶活性、渗透物质等生理特性的影响。结果表明：随干旱胁迫时间的延长，细胞膜透性和丙二醛（MDA）含量不断增加，在干旱胁迫的第20天，细胞膜透性和MDA含量均达到最大，此时植物受害严重。叶片中脯氨酸（Pro）含量迅速增加，复水后又开始下降，膜透性得到了一定的恢复。可溶性蛋白质含量不断下降。复水后，植物的可溶性蛋白质含量有所升高。当胁迫20 d时，其体内可溶性蛋白质含量最低。随着时间的推移，干旱胁迫下植株体内的过氧化物酶（POD）活性、超氧化物歧化酶（SOD）活性均呈先上升后下降的趋势，复水后，POD、SOD活性呈缓慢升高的趋势。第10天时，POD、SOD活性达到最大值，说明此时植物自身有较高的清除活性氧的能力。
Abstract:
Taking Delphinium grandiflorum seedlings as test materials，effect of soil drought stress and rewatering on membrane lipid peroxidation，protective enzyme activity and the permeable material of seedlings were studied.The results showed that with the prolonging of drought stress time,cell membrane permeability and malondialdehyde rose significantly，in section 20 days of drought stress，the cell membrane permeability and malondialdehyde content reached the maximum，the plant was severely damaged.Proline content in leaves increased rapidly，after rewatering began to decline,but the content of soluble protein in the opposite.With the passage of time,the peroxidase and superoxide dismutase activity showed a downward trend after the first rise.After rewatering，peroxidase and superoxide dismutase activity showed a slowly increasing trend.In section 10 days of drought stress,the peroxidase and superoxide dismutase activity reached the maximum，the results showed that the plant had a higher ability of scavenging active oxygen.

参考文献/References:


[1]樊江文,钟华平,陈立波,等.我国北方干旱和半干旱区草地退化的若干科学问题[J].中国草地学报,2007,29(5):95-101.
[2]李广敏,唐连顺,商振清,等.渗透胁迫对玉米幼苗保护酶系统的影响及其与抗旱性的关系[J].河北农业大学学报,1994,17(2):1-5.
[3]彭昌操,孙中海.低温锻炼期间柑桔原生质体SOD和CAT酶活性的变化[J].华中农业大学学报,2000,19(4):384-387.
[4]孔兰静,李红双,张志国.三种观赏草对土壤干旱胁迫的生理响应[J].中国草地学报,2008,30(4):40-45.
[5]池书敏,李广敏,史吉平,等.玉米抗旱机理研究[J].河北农业大学学报,1997,20(4):11-15.
[6]Sergi M B,Lenonor A.Drought-induced changes in the redox state of alpha-tocopherol ,ascorbate and the diterpenecarnosic acid inchloroplasts of labiatae species differing in carnosic acid contents[J].Plant Physiology,2003,131:1816-1825.
[7]Marin C,Boutaleb-Charki S,Diaz J G,et al.Antileishmaniasis activity of flavonoids from Consolida oliveriana[J].Journal of Natural Products,2009,72(6):1069-1074.
[8]刘炳仑.毛莨科部分有毒蜜粉源植物及其花粉形态[J].养蜂科技,1996(3):5-7.
[9]Kokoska L,Urbanova K,Kloucek P,et al.Essential oils in the ranunculaceae family:chemical composition of hydrodistilled oils from Consolida regalis,Delphinium elatum,Nigella hispanica，and N.nigellastrum seeds[J].Chemistry and Biodiversity,2012,9(1):151-161.
[10]韩丽春,许翠英.大花飞燕草对离体器官的作用[J].内蒙古医学杂志,1980,9(1):1-2.
[11]王世毅,何立生,周光创.大花飞燕草肌松作用的研究[J].黑龙江医药,1978(5):16-20.
[12]徐力,刘彦季.飞燕草总黄酮提取物对小鼠炎症抑制作用的研究[J].中国当代医药,2011,18(32):14-59.
[13]吴晓蕾,王松,王素玲.飞燕草的繁育技术及养护管理[J].才智,2011(32):19.
[14]王金发,何小玲,何炎明.大花飞燕草的组织培养及再生体系建立[J].热带亚热带植物学报,2000,8(4):315-318.
[15]张彦妮,李博,何淼.盐胁迫对大花飞燕草种子萌发的影响[J].草业科学,2012,29(8):1235-1239.
[16]张彦妮,李博,何淼.PEG干旱胁迫对大花飞燕草幼苗生理特性的影响[J].草业科学,2014,31(3):446-450.
[17]刘晓东,李洋洋,何淼.PEG模拟干旱胁迫对玉带草生理特性的影响[J].草业科学,2012,29(5):687-693.
[18]Lagerwerff J V.Control of osmotic pressure of culture solution with polyethylene glycol[J].Science,1961,133:1186-1487.
[19]郝再斌,苍晶,徐仲.植物生理试验[M].哈尔滨:哈尔滨工业大学出版社,2004:22-108.
[20]李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2009:184-263.
[21]徐启贺.苹果砧木对干旱胁迫的生理响应及抗旱性评价[D].北京:中国农业科学院,2010.
[22]谢志玉,张文辉,刘新成.干旱胁迫对文冠果幼苗生长和生理生化特征的影响[J].西北植物学报,2010,30(5):948-954.
[23]葛体达,隋方功,白莉平,等.水分胁迫下夏玉米根叶保护酶活性变化及其对膜脂过氧化作用的影响[J].中国农业科学,2005,38(5):922-928.
[24]汤章城.逆境条件下植物脯氨酸的累积及其可能的意义[J].植物生理学通讯,1984(1):15-21.
[25]刘娥娥,宗会,郭振飞,等.干旱、盐、低温胁迫对水稻幼苗脯氨酸含量的影响[J].热带亚热带植物学报,2000,8(3):235-238.

相似文献/References:

[1]方 晶.高压电场对干旱胁迫下黄瓜种子萌发的影响[J].北方园艺,2014,38(13):41.
　FANG Jing.Effect of High Voltage Electric Field on Germination of Cucumber Seeds Under Drought Stress[J].Northern Horticulture,2014,38(09):41.
[2]王贵平,王金政,薛晓敏,等.叶面喷施甜菜碱对干旱胁迫下苹果幼树[J].北方园艺,2014,38(12):10.
　WANG Gui-ping,WANG Jin-zheng,XUE Xiao-min,et al.Effect of Spraying Glycinebetaine on Physiological Responses of Apple Young Trees Under Drought Stress[J].Northern Horticulture,2014,38(09):10.
[3]张 爽,赵 纯,董 然,等.槭叶草对PEG-6000模拟干旱胁迫的生理响应[J].北方园艺,2013,37(22):74.
　ZHANG Shuang,ZHAO Chun,DONG Ran,et al.Physiological Responses of Mukdenia rossii Under PEG-6000 Drought Stress[J].Northern Horticulture,2013,37(09):74.
[4]蔡喜悦,陈晓德,刘成,等.外源钙对干旱胁迫下复羽叶栾树幼苗水分及光合特性的影响[J].北方园艺,2013,37(10):58.
　CAI Xi-yue,CHEN Xiao-de,LIU Cheng,et al.Effect of Drought Stress with Exogenous Ca2+on Relative Water Content and the Characteristics of Photosynthesis of Koelreuteria paniculata[J].Northern Horticulture,2013,37(09):58.
[5]梁蕊芳,康利平,徐 龙,等.干旱胁迫对樱桃番茄幼苗叶片生长特性的影响[J].北方园艺,2013,37(23):12.
　LIANG Rui-fang,KANG Li-ping,XU Long,et al.Effect of Drought Stress on Growth Characteristics of Leaves in Lycopersicom esculentum var. cerasiforme Seedlings[J].Northern Horticulture,2013,37(09):12.
[6]王少平,黄超.切花菊‘黄中黄’对干旱胁迫的生理响应[J].北方园艺,2013,37(15):64.
　WANG Shao-ping,HUANG Chao.Physiological Response of Cut Chrysanthemum ‘Huangzhonghuang’ to Drought Stress[J].Northern Horticulture,2013,37(09):64.
[7]董延龙,常缨.干旱胁迫对五种观赏蕨类植物叶绿素荧光特性的影响[J].北方园艺,2013,37(15):66.
　DONG Yan-long,CHANG Ying.Effects of Drought Stress on Chlorophyll Fluorescence Characteristics of Five Species of Ornamental Ferns[J].Northern Horticulture,2013,37(09):66.
[8]岳桦,石喜梅.聚乙二醇模拟干旱胁迫对兔儿伞生理特性的影响[J].北方园艺,2013,37(11):56.
　YUE Hua,SHI Xi-mei.Effects of Drought Stress Simulated by PEG-6000 on Physiological Characteristics of Syneilesis aconitifolia[J].Northern Horticulture,2013,37(09):56.
[9]邹原东,韩振芹,陈秀新,等.干旱胁迫对蓝羊草渗透调节物质和抗氧化酶活性的影响[J].北方园艺,2013,37(23):71.
　ZOU Yuan-dong,HAN Zhen-qin,CHEN Xiu-xin,et al.Effect of Drought Stress on Osmotic Adjustment Substance and Antioxidative Activity of Leymus chinensis[J].Northern Horticulture,2013,37(09):71.
[10]余莉琳,裴宗平,孔静,等.干旱胁迫下四种矿区生态修复植物的抗旱性研究[J].北方园艺,2013,37(12):61.
　YU Li-lin,PEI Zong-ping,KONG Jing,et al.Drought Resistance of Four Plant Species in Ecological Regeneration on Mining Area Under Drought Stress[J].Northern Horticulture,2013,37(09):61.

备注/Memo

第一作者简介：张彦妮（1974-），女，博士，副教授，研究方向为园林花卉的繁殖栽培及育种。E-mail：ynzhang808@126.com.

基金项目：黑龙江省教育厅科研资助项目（12543012）。

更新日期/Last Update: 2015-08-01