[1]李月荣,颜欢,张波,等.过氧化氢诱导不同色素水平葡萄愈伤组织白藜芦醇及白藜芦醇苷含量差异[J].北方园艺,2015,39(08):114-116.[doi:10.11937/bfyy.201508030]
　LI Yue-rong,YAN Huan,ZHANG Bo,et al.The Differences Between Resveratrol and Piceid in Grapevine Callus Responsed to the Hydrogen Peroxide Treatment[J].Northern Horticulture,2015,39(08):114-116.[doi:10.11937/bfyy.201508030]

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过氧化氢诱导不同色素水平葡萄愈伤组织白藜芦醇及白藜芦醇苷含量差异

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷期数: 2015年08 页码: 114-116 栏目: 出版日期: 2015-04-30

Title:: The Differences Between Resveratrol and Piceid in Grapevine Callus Responsed to the Hydrogen Peroxide Treatment

文章编号:: 1001-0009（2015）08-0114-03

作者:: 李月荣¹; 颜欢¹; 张波^{1 2}; 杨晓燕¹; 李荣飞¹; 王晓琴¹; （1.石河子大学药学院，新疆石河子 832002；2.省部共建新疆特种资源植物药重点实验室，新疆石河子832002）

Author(s):: LI Yue-rong¹; YAN Huan¹; ZHANG Bo^{1 2}; YANG Xiao-yan¹; LI Rong-fei¹; WANG Xiao-qin¹; (1.College of Pharmacy，Shihezi University,Shihezi,Xinjiang 832002;2.Key Laboratory of Xinjiang Endemic Phytomedicine Resources,Ministry of Education,Shihezi，Xinjiang 832002）

关键词:: 葡萄愈伤组织; 白藜芦醇; 白藜芦醇苷; 过氧化氢

Keywords:: grape callus; resveratrol; piceid; H₂O₂

分类号:: S 663.1

DOI:: 10.11937/bfyy.201508030

文献标志码:: A

摘要:: 以不同状态的“红地球”葡萄愈伤组织为试材，采用高效液相色谱法，研究H₂O₂对其白藜芦醇和白藜芦醇苷含量的影响。结果表明：经H₂O₂诱导后3种愈伤组织中白藜芦醇苷含量为：红色愈伤组织>褐化的愈伤组织>白色愈伤组织，而白藜芦醇在白色愈伤组织中的含量高于红色和褐化的愈伤组织。2.5 μM H₂O₂诱导红色愈伤组织24 h后，白藜芦醇苷含量高达306 μg/gFW，而白藜芦醇的含量变化不明显。红色愈伤组织中的白藜芦醇苷响应H2O2的胁迫较为明显，其对H₂O₂最佳的响应浓度为2.5 μM。

Abstract:: Taking three different stages grape callus(Vitis vinifera L.cv.Red Globe) as test materials,with H₂O₂ solution to investigate the effect of resveratrol and piceid content by HPLC.The results showed that the content of piceid in red callus was the highest，following by brown callus，and white callus.The content of resveratrol in white callus was higher than red and browning callus.The maximum content of piceid 306 μg/g FW in treated red callus was achieved by 2.5 μM H₂O₂ for 24 hours treatment.It was not significant that the content of resveratrol changes in three different stages grape callus.It was very obvious that the response of piceid in red callus to H₂O₂，which limited to 2.5 μM H₂O₂.

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备注/Memo

第一作者简介：李月荣（1990-），女，四川威远人，硕士研究生，研究方向为生物技术制药。E-mail：yueronger512@163.com.

责任作者：张波（1978-），男，陕西宝鸡人，博士，教授，研究方向为肿瘤药理及生物技术制药。E-mail：Bozhang_lzu@126.com.

基金项目：国家自然科学基金资助项目（31160058）；新疆兵团重点领域科技攻关资助项目（2014BA029）。

更新日期/Last Update: 2015-07-24