ZHANG Chuan-li,GUI Xue-mei,WANG Xi,et al.Study on the Pleurotus tuber Regium Strains Cultured in Coffee Shell[J].Northern Horticulture,2015,39(07):128-131.[doi:10.11937/bfyy.201507037]
咖啡壳生产“虎奶菇”菌种研究
- Title:
- Study on the Pleurotus tuber Regium Strains Cultured in Coffee Shell
- Keywords:
- Yunnan tropical region; Pleurotus tuber Regium; coffee shell; strains; medium
- 文献标志码:
- A
- 摘要:
- 为拓宽云南热区咖啡主产地可再生资源咖啡壳资源利用途径,探讨咖啡壳制作“虎奶菇”菌种的可能性,为云南热区利用咖啡壳资源制作“虎奶菇”等珍稀食用菌菌种提供科学依据。以“虎奶菇1号”菌种为研究对象,采用单、双、三因素试验设计,以菌丝生长情况及菌种使用效果为评价指标,筛选以咖啡壳作为主要原料生产“虎奶菇”菌种的培养基。结果表明:“虎奶菇”菌丝在9种不同配方的培养基上均能正常生长,其中筛选出配方④(即咖啡壳刨花麸皮培养基:咖啡壳40%、刨花40%、麸皮18%、石膏1%、石灰1%)、配方⑦(即咖啡壳刨花棉籽壳培养基:咖啡壳40%、刨花40%、棉籽壳18%、石膏1%、石灰1%)和配方⑨(即咖啡壳棉籽壳麸皮培养基:咖啡壳40%、棉籽壳40%、麸皮18%、石膏1%、石灰1%)为该试验条件下的最优配方,综合评价指标与对照相差不大。经合理搭配,咖啡壳作为“虎奶菇”菌种生产原料是可行的。
- Abstract:
- In order to broaden the renewable coffee shell resource utilization way in Yunnan tropical,the possibility of production of Pleurotus tuber Regium strain by means of coffee shell as the basic medium was experimented so as to provide scientific references for producing Pleurotus tuber Regium strain of rare edible fungi with coffee shell in Yunnan tropical region.With pleurotus tuber as research object,single,double,three factors experiment was conducted,the indicators of mycelia growth and using effect of Pleurotus tuber Regium was synthetically evaluated for the selection of the coffee shell as main raw materials its medium.The results showed that the Pleurotus tuber Regium mycelia could be normal growth on nine different formula of culture medium,including selected formula ④(namely coffee shell bran medium:coffee shell 40%,wood shaving 40%,wheat bran 18%,1% gypsum,1% lime),formula ⑦(namely coffee shell shavings cotton seed hull medium:coffee shell 40%,wood shaving 40%,cotton seed hull 18%,1% gypsum,1% lime) and formula ⑨ (namely coffee shell cotton seed hull bran medium:coffee shell 40%,the cotton seed hull 40%,wheat bran 18%,1% gypsum,1% lime) for the test under the condition of the optimal formula and comprehensive evaluation index and contrast.The reasonable collocation,coffee shell as raw materials for the Pleurotus tuber Regium strains producing was feasible.
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备注/Memo
第一作者简介:张传利(1981-),男,硕士,讲师,现主要从事生物技术方面的教学与科研工作。E-mail:zhchuanli00@163.com. 基金项目:云南省教育厅资助项目(2012Z131C);云南农业大学热带作物学院科研资助项目(2014KY011)。