YANG Wei-min,DU Jing-qi,ZHAO Jun,et al.Effect of ABA,H2O2 and NO Donor SNP on the Gene Expression and Development of Jujube Fruit[J].Northern Horticulture,2015,39(07):90-93.[doi:10.11937/bfyy.201507027]
ABA、H2O2与NO供体硝普钠对基因表达以及枣果发育的影响
- Title:
- Effect of ABA,H2O2 and NO Donor SNP on the Gene Expression and Development of Jujube Fruit
- Keywords:
- Mu jujube; ABA; H2O2; NO donor SNP; DNA; gene expression
- 文献标志码:
- A
- 摘要:
- 枣在成熟期间遇到连阴雨天气会发生严重的裂果现象，这种基于基因表达的生理病害的发生与非生物或生物胁迫有关。以吕梁当地木枣为试材，在木枣成熟期，采用改良CTAB法检测组织中DNA纯度及含量的变化，研究ABA、H2O2与NO供体硝普钠(SNP)在枣果发育中对基因表达的影响。结果表明:ABA、H2O2与NO供体硝普纳（SNP）对枣果发育中DNA纯度及含量的影响不一，差异较为明显。其中不同浓度的ABA处理枣果组织中DNA的OD260/OD280比值基本不在1.700~1.900范围内，DNA纯度差异明显。但样品DNA含量较高，其值在1 200 ng/μL左右；H2O2 和SNP处理样品DNA 的OD260/OD280比值基本上在1.700~1.900之间，DNA纯度较高。样品DNA含量基本上小于或在1 000 ng/μL左右。外源ABA较为明显地干扰了基因表达，诱导了新蛋白质、RNA和酚类物质等的生物合成，有促进木枣果发育的趋势；而H2O2和NO供体硝普钠(SNP)对基因表达或枣果发育的影响不十分明显。
- Abstract:
- The jujube encounter overcast and rainy weather occurs the phenomenon serious in the mature period,the physiological disease gene expression associated with abiotic or biotic stress based on.Taking Lyuliang local Mu jujube as test material,in the jujube mature period,detection for change of DNA purity and contentby of tissue by the modified CTAB method, gene expression of ABA,H2O2 and NO in fruit development were studied.The results showed that effect of ABA，H2O2 and NO donor sodium nitroprusside (SNP) on purity and conten of DNA in fruit development had significant difference.Treated with different concentrations of ABA in jujube fruit，the DNA OD260/OD280 ratio was not exactly in the range of 1.700-1.900，the purity of DNA was obvious difference.But the content of DNA was higher, its value at 1 200 ng/μL; the DNA OD260/OD280 ratio was basically in between 1.700-1.900 with H2O2 and SNP treated，DNA with high purity. The content of DNA in the sample basically or less than or at 1 000 ng/μL.The gene expression were obviously interfered with exogenous ABA,and induced biosynthesis of new proteins，RNA，and the phenolic compounds，had a tendency to promote the development of jujube fruit；While effect of H2O2 and NO on gene expression or in fruit development was not very obvious.
参考文献/References:
[1]程国媛，程春生.山西红枣防裂果技术取得突破[N].中国绿色时报,2013-05-16. [2]杨卫民,杜京旗,赵君,等.ABA与NO,GA 在枣果实发育期的网络关系与拮抗效应探讨[J].山西农业科学,2014,42(2)：195-198,202. [3]周运刚,张静,郑新疆.红枣裂果的研究进展[J].江西农业学报,2012,24(7):25-27. [4]李登科,黄丛林,田建保,等.高质量枣树基因组DNA提取方法的研究[J].分子植物育种,2005,3(4):579-583. [5]陈碧华.一种改良的CTAB法提取马尾松基因组DNA[J].广东林业科技，2009,25(2):26-29. [6]罗志勇，周钢，陈湘晖，等.高质量植物基因组DNA 的分离[J].湖南医科大学学报，2001,26(2)：178-180. [7]周延清.DNA分子标记技术在植物研究中的应用[M].北京:化学工业出版社,2005:43-44. [8]易文凯,王佳,杨辉,等.植物ABA受体及其介导的信号转导通路[J].植物学报,2012,47(5):515-524. [9]Thomas S G，Franklin-Tong V E.Self-incompatibility triggers programmed cell death in Papaver pollen[J].Nature,2004，429:305-309. [10]Lin A,Wang Y Q,Tang J Y,et al.Nitric oxide and protein S-nitrosylationare integral to hydrogen peroxide-induced leaf celldeath in rice[J].Plant Physiol,2012,158:451-464. [11]Torres M A,Jones J D G,Dangl J L.Reactive oxygen species signaling in response to pathogens[J].Plant Physiol,2006,141:373-378.
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备注/Memo
第一作者简介：杨卫民（1960-），男，山西文水人，本科，教授，现主要从事枣裂果调控机制等研究工作。E-mail:yangweimin0318@sina.com. 基金项目：山西省自然科学基金资助项目（2013011029-1）；吕梁学院自然科学校内基金资助项目（ZRXN201203）。