WANG Yu-meng,SHI Dong-fang,LI Sai-nan,et al.Optimize of Extraction Technology of Juglone from Juglandis mandshuricae Cortex[J].Northern Horticulture,2015,39(05):141-144.[doi:10.11937/bfyy.201505041]
核桃楸树皮中胡桃醌提取工艺优化
- Title:
- Optimize of Extraction Technology of Juglone from Juglandis mandshuricae Cortex
- Keywords:
- tree bark of Juglandis mandshuricae Cortex; high performance liquid chromatography; juglone; orthogonal test
- 文献标志码:
- A
- 摘要:
- 以核桃楸树皮为试材，采用正交实验的方法对核桃楸树皮进行提取，并用HPLC技术对其化学成分进行检测，应用标准曲线法计算胡桃醌的含量。结果表明：从核桃楸树皮中提取胡桃醌的最佳工艺料液比1∶12 g/mL，乙醇浓度95%，回流提取2次，每次回流50 min。
- Abstract:
- Taking tree bark of Juglandis mandshuricae Cortex as the test material and the factors about the extraction by set up L9(34) orthogonal test was optimized.The content of juglone was calculated by HPLC technique combined with the method called standard curve.The results showed that optimum extraction conditions of juglone from Juglandis mandshuricae Cortex was solid-liquid ratio of 1∶12 g/mL，the ethanol concentration of 95%，extracted two times，each time the reflux of 50 min.
参考文献/References:
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备注/Memo
第一作者简介：王雨萌（1989-），女，硕士研究生，研究方向为天然药物化学。E-mail:dongfang1107@163.com. 责任作者：刘春明(1964-)，女，博士，教授，现主要从事天然药物化学等研究工作。基金项目：吉林省发改委资助项目（2013C002）；吉林省教育厅资助项目（吉教科合字[2012]第225号）；长春师范学院自然科学基金资助项目（长师院自科合字[2012]第05号）。