WANG Xiao-lin,ZHONG Fang-li,XUE Jian-fei,et al.Study on the Extraction Technology of Total Flavonoids in Fruit of Rosa davurica Pall.with Enzymes Method[J].Northern Horticulture,2015,39(04):136-139.[doi:10.11937/bfyy.201504032]
酶法提取刺玫果总黄酮工艺研究
- Title:
- Study on the Extraction Technology of Total Flavonoids in Fruit of Rosa davurica Pall.with Enzymes Method
- Keywords:
- fruit of Rosa davurica Pall.; total flavonoids; enzymolysis; extract
- 文献标志码:
- A
- 摘要:
- 以刺玫果为试材,采用酶法辅助提取刺玫果总黄酮,研究各因素对刺玫果总黄酮提取率的影响。结果表明:纤维素酶辅助提取刺玫果总黄酮的最佳工艺条件为提取溶剂为50%乙醇、温度控制在50℃、料液比1∶15 g/mL、纤维素酶加入量为40 mg/g、控制提取液于pH 5.5条件下,酶解提取2次,每次140 min,在最佳条件下进行3次验证试验,其总黄酮提取率平均为95.94 mg/g。
- Abstract:
- Taking fruits of Rosa davurica Pall. as test materials,the effect of many factors on the extraction efficiency of total flavonoids(TF) in fruits of Rosa davurica Pall. with enzymes-aided extraction method were studied.The results showed the optimum extraction technology with cellulase as follow,the concentration of the ethanol extraction solvent,the temperature,the ratio between raw material and solvent,the quantity of cellulase,the pH value,the extraction times and time were 50%,50℃,1∶15 g/mL,40 mg/g,5.5,2 times,140 min respectively.The average extraction efficiency of TF was 95.94 mg/g in three verification experiments under the optimized extraction condition.
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备注/Memo
第一作者简介:王晓林(1969-),男,山东五莲人,硕士,副教授,现主要从事天然产物有效成分的提取及纯化工艺等研究工作。E-mail:wangxiaolin69@eyou.com.责任作者:钟方丽(1970-),女,山东安丘人,博士,教授,硕士生导师,现主要从事天然产物化学成分的分离与生物活性等研究工作。E-mail:fanglizhong@sina.com.基金项目:吉林省科技厅计划资助项目(20110948)。