LI Jin,GENG Jin-peng,CAO Tian-guang,et al.Study on the Mutagenic Effects of Space Environment on Cyclamen persicum Mill.[J].Northern Horticulture,2015,39(04):112-115.[doi:10.11937/bfyy.201504026]
太空环境对仙客来诱变效应的研究
- Title:
- Study on the Mutagenic Effects of Space Environment on Cyclamen persicum Mill.
- 关键词:
- 太空环境; 仙客来; 表观性状; 突变株; 随机扩增多态性DNA
- Keywords:
- space environment; Cyclamen persicum Mill.; phenotypic traits; mutants; random amplified polymorphic DNA analysis
- 文献标志码:
- A
- 摘要:
- 以仙客来干种子为试材，利用“神舟八号”飞船搭载进行太空诱变处理，以此探究太空环境对仙客来造成的诱变效应。结果表明：太空诱变产生的M1代仙客来植株在花色和花形上与对照株相比表现出明显的表观性状差异；对突变株进行的RAPD（Random amplified polymorphic DNA）试验分析中，28条随机引物中有27条引物扩增出现多态性，总共扩增出156条条带，其中91条呈现出多态性，多态性比率达到58.33%。所有突变株的DNA分子均发生了显著变化，变异率从23.08%到33.97%不等。综合分析显示，太空环境对当代植株造成了显著的诱变效应，经辐照后的植株在分子水平上均发生了明显的变异，而基因组的改变进一步导致了植株表观性状的变异。
- Abstract:
- To explore the mutagenic effect of space environment on Cyclamen persicum Mill.，dry seeds were carried by Chinese“Shen Zhou VIII”.After recovery，these seeds were planted and investigated.The results showed the first generation of the space-exposed plants displayed considerable changes of phenotypic traits in flower color and flower shape compared with their control plants.Mutated plants which acquired new phenotypic traits after space flight were analyzed by random amplified polymorphic DNA (RAPD) analysis.Among 28 random primers used in this experiment，27 primers generated different DNA band type.156 DNA bands were produced，among which 91 DNA bands were polymorphic with the percentage of polymorphism being 58.33%.The DNA of mutated plants had changed，and the DNA mutation rates varied from 23.08% to 33.97%，which suggested that dramatic changes on DNA molecular level occurred after the plants had been exposed to the space environment.Results of the study demonstrated that the mutagenic effects could occur in the seeds flown in space，and the space environment could make changes in some of the genes，and these changes in the genes would induce phenotypical changes.
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备注/Memo
第一作者简介：李谨（1986-），女，博士研究生，研究方向为辐照生物学。E-mail：Davidjin2013@163.com.责任作者：展永（1954-），男，博士，教授，博士生导师，现主要从事生物大分子动力学及辐照生物学等教学与研究工作。E-mail：Yong_zhan@163.com.基金项目：河北省科技计划资助项目（09220102D）；河北省自然科学基金资助项目（2013202192）。