[1]马玉琳,谷娜,池惠荣,等.复方二氧化氯对平菇褐斑病病原菌的杀菌效果及机理[J].北方园艺,2015,39(03):130-134.[doi:10.11937/bfyy.201503038]
　MA Yu-lin,GU Na,CHI Hui-rong,et al.The Germicidal Efficacy and Mechanism of a Compound Chlorine Dioxide to Pathogen of Oyster Mushroom Brown Spot[J].Northern Horticulture,2015,39(03):130-134.[doi:10.11937/bfyy.201503038]

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复方二氧化氯对平菇褐斑病病原菌的杀菌效果及机理

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷期数: 2015年03 页码: 130-134 栏目: 出版日期: 2015-02-15

Title:: The Germicidal Efficacy and Mechanism of a Compound Chlorine Dioxide to Pathogen of Oyster Mushroom Brown Spot

作者:: 马玉琳1; 谷娜2; 池惠荣3; 王奎涛4; （1.河北科技大学化学与制药工程学院，河北石家庄 050018；2.河北科技大学理学院，河北石家庄 050018；3.河北省农业科学院遗传生理研究所，河北石家庄 050050；4.河北省药用分子化学重点实验室，河北石家庄 050018）

Author(s):: MA Yu-lin1; GU Na2; CHI Hui-rong3; WANG Kui-tao4; (1.School of Chemical and Pharmaceutical Engineering，Hebei University of Science and Technology，Shijiazhuang，Hebei 050018；2.School of Science，Hebei University of Science and Technology，Shijiazhuang，Hebei 050018；3.Institute of Genetics and Physiology，Hebei Academy of Agricultural Sciences,Shijiazhuang，Hebei 050050；4.Key Laboratory of Medicinal Molecular Chemistry in Hebei Province，Shijiazhuang，Hebei 050018)

关键词:: 食用菌; 细菌性病害; 褐斑病; 复方二氧化氯; 杀菌

Keywords:: edible mushroom; bacterial disease; brown spot; compound chlorine dioxide; sterilization

DOI:: 10.11937/bfyy.201503038

文献标志码:: A

摘要:: 以平菇为试材，将采集到的平菇褐斑病标本进行了细菌菌株的分离纯化，纯化后的细菌菌株经回接感染试验和细菌形态学鉴定确定致病病原菌。通过悬液定量杀菌试验研究不同配方复方二氧化氯食用菌杀菌剂对平菇褐斑病病原菌的杀菌效果，筛选出有效的复方二氧化氯食用菌杀菌剂配方。最后从细菌细胞微观形态和细胞膜渗透性变化2个方面分析了复方二氧化氯的杀菌机理。结果表明：5 mg/L二氧化氯和2.3 mg/L的烷基糖苷的复配后对平菇褐斑病病原菌杀灭对数值可以达到5，杀菌作用显著。复方二氧化氯中的杀菌增效剂烷基糖苷对二氧化氯杀菌起到协同增效作用，可以降低二氧化氯的用量，复方二氧化氯对病原菌细胞超微结构和内含物的破坏能力大于二氧化氯，杀菌效果更好。

Abstract:: Taking oyster mushroom as test material，the bacteria strains were isolated from oyster mushroom infected by brown spot and pathogenic bacteria were indentified by artificial infection test and morphological identification.The optimum ingredient design of compound chlorine dioxide was determined by studying the bactericidal effects of bactericide with different ingredients on pathogenic bacteria of brown spot through using suspension quantitative germicidal test.At last the germicidal mechanism of compound chlorine dioxide was analyzed by the variation of micro morphology of bacterial cell determined by TEM and permeability of cell membrane indicated by soluble protein.The results showed that the killing log value of pathogen of oyster mushroom brown spot exposed to optimum compound ingredient with 5 mg/L chlorine dioxide disinfectant and 2.3 mg/L alkyl-polyglucoside(APG) could attain 5.The use of sterilization synergist APG in compound chlorine dioxide could decre-ase the dosage of chlorine dioxide thus reduce the affection to fruitbody.The damaged capacity of compound chlorine dioxide to micromorphology and inclusion of bacteria cells was more than chlorine dioxide.

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备注/Memo

第一作者简介：马玉琳（1989-），女，硕士研究生，研究方向为食用菌病害防治。E-mail：804066070@qq.com.责任作者：王奎涛（1962-），男，教授，研究方向为食用菌病害防治。E-mail：xiaoyuan41@126.com.基金项目：河北省科技支撑计划资助项目（11230908D-4-4）。

更新日期/Last Update: 2015-03-03