XU Dong-sheng.Study on Extraction Total Flavonoids by Microwave from Astragalus membranaceus[J].Northern Horticulture,2015,39(03):113-115.[doi:10.11937/bfyy.201503033]
微波提取黄芪黄酮的方法研究
- Title:
- Study on Extraction Total Flavonoids by Microwave from Astragalus membranaceus
- Keywords:
- Astragalus membranaceus; microwave; flavonoid
- 文献标志码:
- A
- 摘要:
- 以黄芪为试材，用微波消解仪提取黄酮，分光光度法测定黄酮提取率，采用单因子试验及正交实验研究黄芪黄酮的最佳提取条件。结果表明：乙醇浓度95%，提取时间20 min，料液比1∶15 g/mL，提取温度90℃时，提取效果最好，黄酮提取率为0.489%，各因子对提取效果影响从大到小依次为提取温度>乙醇浓度>料液比>提取时间。
- Abstract:
- Taking the Astragalus membranaceus as material and ethanol as solvent to extract total flavonoids with microwave digestion instrument，the optimal condition of extraction was studied through the orthogonal test based on the single factor,total flavonoid content were determinated by spectrophotometry.The results showed the optimal extraction conditions were as follows：ethanol concentration 95％，extraction time 20 minutes，solid to liquid ratio 1∶15 g/mL，temperature 90℃，flavonoids extraction rate was 0.489%，the order of affecting was the temperature>ethanol concentration>solid to liquid ratio>extraction time.
参考文献/References:
[1]李淑芳.中药黄芪药理作用研究进展[J］.湖北中医杂志,2013,35(6):73-75.
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备注/Memo
作者简介：徐东生（1955-），男，湖北武汉人，高级实验师，现主要从事植物及植物保护等研究工作。E-mail:1377994531@qq.com.基金项目：湖北省重点实验室开放基金资助项目（2014K09）。