HAN Xue-ping,XUE Xiao-min,WANG Jin-zheng,et al.Screening Factors That Impact Pollen Growth of Prunus armeniaca L.cv ‘Katy’ in vitro[J].Northern Horticulture,2015,39(03):96-99.[doi:10.11937/bfyy.201503029]
‘凯特’杏花粉离体培养条件筛选
- Title:
- Screening Factors That Impact Pollen Growth of Prunus armeniaca L.cv ‘Katy’ in vitro
- Keywords:
- Prunus armeniaca L.cv ‘Katy’; pollen germination; sucrose; boric acid
- 文献标志码:
- A
- 摘要:
- 以‘凯特’杏为试材，采用不同培养基组分及不同培养基类型的方法，测定了花粉萌发率和花粉管伸长长度，研究了不同培养基组分及不同培养基类型对花粉萌发和花粉管伸长的影响。结果表明：固体培养基适合‘凯特’杏离体萌发，最适培养基组分是10%蔗糖+0.10%硼酸+0.5%琼脂；在该培养基上，花粉萌发率达90%以上，花粉管生长长度为13 510.30 μm。
- Abstract:
- Taking Prunus armeniaca L.cv ‘Katy’ as material，the pollen germination rate and the pollen tube length that different types of mediums and different concentrations of sucrose and boric acid were determinated.The effect of different types of mediums and different concentrations of sucrose and boric acid for pollen germination and pollen tube elongation were studied.The results showed that,most suitable medium for Prunus armeniaca L.cv ‘Katy’ was 10% sucrose+0.10% boric acid+0.5% agar.It was more than 90% pollen germination rate and the length of pollen tube growth 13 510.30 μm in vitro.
参考文献/References:
[1]黄家兴,吴杰,安建东,等.凯特杏花粉的离体培养及影响因子分析[J］.西北植物学报，2008，28(1)：52-58.
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备注/Memo
第一作者简介：韩雪平（1985-），女，硕士，研究实习员，现主要从事核果类育种等研究工作。E-mail：hanxuepingrun@163.com.责任作者：薛晓敏（1979-)，女，硕士，助理研究员，现主要从事果树育种与栽培等研究工作。基金项目：国家“十二五”科技支撑计划资助项目（2013BAD02B03-3-3）。