LI Qian-nan,TIAN Li-ping,ZHANG Yi-hui,et al.The Seed Melon Optimum Extraction Technology of Total Saponins[J].Northern Horticulture,2015,39(01):140-142.[doi:10.11937/bfyy.201501037]
籽瓜总皂苷的提取工艺研究
- Title:
- The Seed Melon Optimum Extraction Technology of Total Saponins
- Keywords:
- Keywords:seed melon; saponin; extraction
- 文献标志码:
- A
- 摘要:
- 摘 要：以籽瓜为试材，通过乙醇回流法、超声法、超声纤维素酶法、超声果胶酶法提取籽瓜总皂苷，并采用4因素3水平正交实验设计对超声纤维素酶法提取工艺进行优选，以籽瓜提取液中总皂苷的含量和提取物得率为指标，优选最佳提取工艺。结果表明：纤维素酶用量为10 U/g，pH 4.5，酶解温度为45℃，酶反应时间为120 min为籽瓜总皂苷提取的最佳工艺条件。籽瓜提取物得率为61.32%，籽瓜总皂苷的含量为5.03%，该试验操作简单，工艺条件稳定。
- Abstract:
- Abstract:Taking seed melon as material，the ethyl alcohol，transonic extraction，transonic cellulose，pectinase transonic extraction on total saponins were studied by orthogonal test of 4 factors and 3 levels,and with the index of total saponins and extraction rate,the optimum technology was studied.The results showed that cellulase dosage of 10 U/g，pH 4.5，reaction temperature was 45℃，enzyme reaction time was 120 minutes were the optimal technogical condition.Seed melon extract yield was 61.32%；seed melon of total saponin content was 5.03%.This experimental operation was simple，stable process conditions.
参考文献/References:
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备注/Memo
第一作者简介：李欠南（1987-），女，硕士，研究方向为药物资源的开发利用与研制。E-mail：295522152@qq.com.责任作者：田丽萍（1961-），女，教授，硕士生导师，现主要从事植物化学和资源植物等研究工作。E-mail：Li-pingt@163.com.基金项目：兵团科技援疆计划资助项目（2012 AB013，2012-2014）。