WANG Chang-xian,XIAO Long.Effect of Different Nitrogen Forms on Growth and Nutrients Uptake of Cucumber Seedlings in Sand Culture[J].Northern Horticulture,2015,39(01):8-11.[doi:10.11937/bfyy.201501003]
不同氮形态对沙培黄瓜幼苗生长及养分吸收的影响
- Title:
- Effect of Different Nitrogen Forms on Growth and Nutrients Uptake of Cucumber Seedlings in Sand Culture
- Keywords:
- Keywords:cucumber; NO3--N; NH4 +-N; growth; nutrients; nitrate reductase; free amino acid
- 文献标志码:
- A
- 摘要:
- 摘 要：以黑龙江主栽黄瓜品种“津春3号”为试材，采用盆钵培养的方法，研究了营养液中不同NO3--N与NH4 +-N配比对黄瓜幼苗生长及氮、磷养分吸收的影响。结果表明：沙培条件下，营养液中氮的总浓度为15 mmoL/L时，随着营养液中NH4 +比例的增加，幼苗长势随之变弱；全NO3-N处理的幼苗叶面积、干重及壮苗指数分别是全NH4 +-N处理的1. 92、2. 12与3. 07倍；氮形态对幼苗地上部全氮含量与硝酸还原酶活性无显著影响，而对幼苗地上部全磷含量影响显著，全NO3--N处理的幼苗磷含量显著高于全NH4 +-N处理，为全NH4 +-N处理的2倍；受NH3毒害，全NH4 +-N处理的幼苗根系活力显著下降，地上部游离氨基酸含量显著上升。全NO3-处理对黄瓜幼苗生长最有利。
- Abstract:
- Abstract:Taking ‘Jinchun No. 3’ which was main cucumber variety of Heilongjiang as materials,a pot experiment was conducted to investigate the effect of ratio of NO3--N to NH4 +-N on cucumber seedling growth and N and P uptake.The results showed that maintaining the total N concentration in solution at 15 mmoL/L,seedlings became weak in growth with the increasing of NH4 +-N ration in sands.The leaf area,dry weight and the indexes of seedling’s quality treated by NO3--N were 1. 92 times,2. 12 times and 3. 07 times higher than NH4 +-N treatment.The effect of ratio of NO3--N to NH4 +-N on N content and nitrate reductase activity were not significant；however,the effect on P content was significant,the P content in shoots treated by NO3--N was 2 times higher than NH4 +-N treatment.For the damage caused by NH3,the root activity of seedlings treated by NH4 +-N decreased significantly,but increase in free amino acid content was detected.The quality of seedlings treated by NO3--N was the best.
参考文献/References:
[1] 陆景陵.植物营养学(上册)[M].北京:中国农业大学出版社,2002:23-25.
[2] 高祖明,张春兰,倪金应,等.黄瓜等九种蔬菜与NO3--N亲和力的研究[J].南京农业大学学报,1990,13(1):75-79.
[3] 寿森炎,楼惠宁,董伟敏.氮素形态不同配比对黄瓜生长和性别分化的影响（初报）[J].园艺学报,1996,23(1):49-53.
[4] 武新岩,郭建华,方正,等.不同氮素形态对黄瓜光合作用及果实品质的影响[J].华北农学报,2011,26(2):223-227.
[5] 杜永臣,张福墁,刘步洲.不同形态氮素对温室沙培黄瓜生长发育的影响[J].园艺学报,1989,16(1):45-50.
[6] 吕桂云,陈贵林,齐国辉.黄瓜菌根化育苗基质的研究[J].中国蔬菜,2002(4):9-12.
[7] 李合生.植物生理生化实验原理与技术指导[M].北京:高等教育出版社,2000:1-278.
[8] 鲍士旦.土壤农化分析[M].北京:中国农业大学出版社,2002.
[9] 郝建军,康宗利,于洋.植物生理学实验技术[M].北京:化学工业出版社,2006:175-176.
[10] Crasford N M,Glass A D M.Molecular and physiological aspects of nit rate uptake in plants[J].Trends Plant Science,1998,3(10)：389-395.
[11] Andrews M.The partitioning of nitrate assimilation between root and shoot of higher plants[J].Plant Cell and Environ,1986,9(7):511-519.
[12] Haynes R J.Uptake and assimilation of mineral nitrogen by plants[A]//Haynes R J (ed).Mineral nitrogen in the plant soil system[M].Orlando：Academic Press,1986:303-378.
[13] Nicodemus M A,Salifu K F,Jacobs D F.Growth,nutrit ion,and photosynthetic response of Juglans nigra to varying nitrogen source and rate[J].J Plant Nutr,2008,31:1917-1936.
[14] 高青海,魏珉,杨凤娟,等.黄瓜幼苗干物质积累、膨压及光合速率对铵态氮和硝态氮的响应[J].植物营养与肥料学报,2008,14(1):120-125.
备注/Memo
第一作者简介：王倡宪（1974-），女，博士，副教授，现主要从事菌根生物技术等教学与科研工作。E-mail：wangchangxian@hlju.edu.cn.基金项目：黑龙江省教育厅科学技术研究资助项目（12511403）。