WANG Chang-tong,CAO Gang-qiang,LIANG Cong-min,et al.Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber[J].Northern Horticulture,2014,38(14):101-104.
黄瓜NBS类抗病基因类似物的生物信息学分析
- Title:
- Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber
- 文章编号:
- 1001-0009(2014)14-0101-04
- Keywords:
- cucumber; NBS; RGA; bioinformatics
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 利用生物基因组学数据库，对黄瓜NBS类抗病基因类似物(RGA基因)进行生物信息学分析，以预测RGA基因编码产物的理化性质、结构与功能，为进一步研究黄瓜抗病调控机制提供一定的理论依据。结果表明：RGA基因编码产物为一个长度达84个氨基酸的蛋白，其理论分子量为10 964.32 Da，理论等电点为PI 9.36，不存在跨膜区域，主要定位在细胞质中；进化比对表明，黄瓜的NBS类抗病基因在亲缘关系上与西瓜最近，其次是丝瓜；二级结构为α-β型，具有较为明显的3个螺旋和2个折叠；具有酰化位点、酪蛋白激酶II磷酸化位点和蛋白激酶C磷酸化位点3个功能位点；预测RGA基因编码产物主要充当辅酶角色参与生化反应的调控，介导基因表达调控，在抗逆相关的信号通路以及种子胚的发育等过程中发挥重要的生理功能。
- Abstract:
- The bioinformatics of cucumber NBS resistance gene analogs (RGA gene) was analyzed using bioinformatics software (NCBI,DNAMAN,nnPredict,Antheprot,SOSUI,CBS,SWISS-MODEL).The physical and chemical properties,structure and function of the cucumber NBS resistance gene analogs (RGA gene) protein were predicted,in order to provide a theoretical basis for the further study on the regulation mechanism of disease resistance of cucumber.The results showed that the RGA gene encoded a length of 84 amino acid protein.Its molecular weight was 10 964.32 Da.The theoretical isoelectric point was 9.36.This gene had no transmembrane domain.It mainly was located in the cytoplasm.Evolutionary analysis indicated that the cucumber NBS resistance genes had the close genetic relationship with watermelon,also loofah.The secondary structure on this gene was α-β type,with the more obvious three helices and two fold.The protein had three functional sites:N-myristoylation site,Casein kianse II phosphorylation site and Protein kinase C phosphorylation site.And predicted that RGA protein could acts as a coenzyme role in the regulation of biochemical reactions,mediated regulation of gene expression in the stress resistant associated signaling pathways,as well as it could play an important physiological function during the development of seed embryos.
参考文献/References:
[l] Whitham S, Dinesh-Kumar S P,Choi D, et a1.The product of the tobacco mosaic m.rus resistance gene N: Similarity to toll and the interleukin-l receptor[J]. Cell,1994, 78(6): 1101-1115.
[2]李峰，张颖，刘崇怀，等，植物NBS-LRR类抗病基因的研究进展[J].分子植物育种（网络版），2011(9)：1784-1790.
[3]高丽华，周以飞，郑伟文，等．南瓜NBS类抗病基因同源序列的克隆与分析[J]．长江蔬菜，2007(8)：40-43.
[4] Collins N C,Webb C A,Seah S. The isolation and mapping of disease resistance gene analogs in maize[J]. Molecular Plant-microbe Interact, 1988(II)：968-978.
[5]秦跟基，李万隆，陈佩度，等，植物抗病基因结构特征及其类似序列的研究进展[J]．南京农业大学学报，1999 ,22(3)：102-107.
[6] Deng Z, Huang S, Ling P, et aL Cloning and characterization of NBS-LRR class resistance-gene candidate sequences in citrus [J]. Theoretical and Applied Genetics, 2000 ,101(5-6): 814-822.
[7] Zhou T, Wang Y, Chen J Q, et al.Genome-wide identification of NBSgenes in japonica nce reveals significant expansion of divergent non-TIR NBS-LRR genes[J]. Mol Genet Genomics, 2004, 271: 402-415.
[8]薜勇彪，唐定中，张燕生，等，水稻基因组中R类抗病基因同源序列的分析[J].科学通报，1998,43(2)：277-281.
[9] Meyers B C,DickermanA W, Michelmore R W, et al.Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily[J]. Plant,1999, 20(3): 317-332.
[10] Brotman Y.Silberstein L,Kovalski I,et al. Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance[J].Theoretical and Applied Genetics.2002 ,104(6-7):1055-1063.
[11] Gao Y, Guo W, Wang L, et al.Isolation and characterization of resist-ance and defense gene analogs in ootton (Gossypium barbadenseL)[J].Science in China Series C, Life Sciences, 2006, 49(6):530-542.
[12] Huang X, Xu Z Q, Chen LY, et al.Cloning and identification of theResistance gene analogs of wheat[J]. Journal of Molecular Cell Biology, 2006,39(2)：91-96.
[13] Sela H,Cheng J,Jun Y, et al.Divergent diversity patterns of NBS and LRR domains of resistance gene analogs in wild emmer wheat populations[J]. Genome, 2009, 52 (6): 557-565.
[14] Liu J J ,Ekramoddoullah A K M Isolation,genetic variation and expres-sion of TIR-NBS-LRR resistance gene analogs from western white pine( Pinus monticola Dougl. exn Don) [J]. Mol Genet Genomics, 2003, 270 (5):432-441.
[15]张春宝，赵丽梅，赵洪锟，等．植物蛋白激酶研究进展[J]．生物技术通报，2011(10)：17-23.
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备注/Memo
第一作者简介：王昶童(1988-),男,硕士研究生,研究方向为抗冷黄瓜选育。E-mail:king_pose@126.com.