LIN Ying,SI Chun-can,ZHAO Qing-song,et al.Isolation and Identification of A Bacillus vallismortis CZ and Primary Testing Its Disease-suppressive and Growth-promoting Capacities[J].Northern Horticulture,2014,38(13):88-92.
一株死谷芽孢杆菌的分离、鉴定及抗病促生效果初探
- Title:
- Isolation and Identification of A Bacillus vallismortis CZ and Primary Testing Its Disease-suppressive and Growth-promoting Capacities
- 文章编号:
- 1001-0009（2014）13-0088-05
- 分类号:
- S 476
- 文献标志码:
- A
- 摘要:
- 为了寻求新的生物防治菌种资源，以醋糟基质为试验材料，采用稀释涂布平板法从醋糟中分离出对立枯丝核菌具有明显抑制作用的拮抗菌，采用平板对峙法研究其对病原菌的广谱抑制效果,将菌体特征与16S rDNA序列分析及生理生化指标相结合，对拮抗菌进行初步鉴定，确定其分类地位，采用温室盆栽试验对分离的拮抗菌进行抗病促生效果初探。结果表明：从醋糟中分离到了1株对立枯丝核菌具有明显拮抗作用的菌株CZ,且菌株CZ具有广谱的抑菌效果，对西瓜枯萎病、香瓜枯萎病、黄瓜枯萎病、水稻恶苗病、小麦赤霉病、苹果炭疽病原菌都具有较强的拮抗作用。根据菌体特征、16S rDNA序列分析及生理生化指标的结果，初步鉴定该菌为死谷芽孢杆(Bacillus vallismortis CZ)。温室盆栽试验表明菌株CZ对由立枯丝核菌引起的黄瓜立枯病具有显著的抑制效果，与对照组相比，接种菌株CZ后，黄瓜小苗的病情指数降低了18.3%。由此可见，菌株Bacillus vallismortis CZ具有开发成生防试剂的潜力。
- Abstract:
- In order to seek new biocontrol agents，taking the vinegar residue substrate as the test materials，bacteria isolated from the vinegar residue substrate with inhibitory activities toward R.Solani using the dilution plate method，their broad antifungal inhibitory activity were tested using the dual plate assay,combining with morphological characteristics,16S rDNA sequence and physiological and biochemical characteristics to identify and determine its taxonomic status，its antagonistic potential against disease and plant growth promotion under greenhouse were further evaluated.The results showed that strain CZ isolated from the vinegar residue substrate exhibited broad-spectrum antagonism towards the tested fungi，including Fusarium oxysporum f.sp.niveum，Fusarium oxysporum f.sp.Melonis，Fusarium oxysporum f.sp.cucumerinum，Gibberella fujikuroi (Sawada) Wollenw，Fusarium graminearum，Glomerella cingulate.According to the morphological，phylogenetic analysis of the 16S rDNA sequence and physiological characteristics，strain CZ was identified as Bacillus vallismortis.Strain CZ also was found to be effective in controlling damping-off disease caused by Rhizoctonia solani under greenhouse conditions，inoculated with strain CZ led the percent disease index reductions in 18.3%.Together all，strain CZ has great potential as a biocontrol agent.
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备注/Memo
第一作者简介：林英(1985-)，女，博士研究生，研究方向为设施园艺工程与技术。E-mail:jslinying@163.com.