LIU Ji-ping,ZHANG Zhao-qi,LI Hong-mei,et al.Pretreatments With Nano-silver and STS Alleviated the Harmful Effect of Exogenous Ethylene on Cut Waxflowers[J].Northern Horticulture,2014,38(12):112-115.
纳米银和硫代硫酸银预处理对减轻蜡花切花乙烯伤害的研究
- Title:
- Pretreatments With Nano-silver and STS Alleviated the Harmful Effect of Exogenous Ethylene on Cut Waxflowers
- 文章编号:
- 1001-0009(2014)12-0112-04
- 关键词:
- 蜡花切花; 纳米银; 硫代硫酸银(STS); 乙烯; 花朵脱落
- Keywords:
- cut waxflower; nano-silver; silver thiosulfate; ethylene; flower abscission
- 分类号:
- S 681.5
- 文献标志码:
- A
- 摘要:
- 以乙烯敏感型切花蜡花为试材,用10、20 mg/L纳米银(nano-silver,NS)溶液及0.5 mmol/L硫代硫酸银(silver thiosulfate,STS)溶液分别预处理蜡花切花茎基端24 h,以去离子水为对照,随后移至去离子水中瓶插,并用5 μL/L外源乙烯处理24 h,观测瓶插期间的观赏品质、瓶插寿命和花朵脱落率。结果表明:乙烯处理可加快蜡花切花失水凋萎及花朵脱落,并抑制花朵开放,而NS和STS预处理可显著减轻乙烯处理对蜡花切花的不利影响,其中以0.5 mmol/L STS处理效果最佳,10 mg/L NS处理次之;另外,采用电感耦合等离子体-原子发射光谱(ICP-AES)法测定10 mg/L NS和0.5 mmol/L STS溶液预处理后银(Ag)在蜡花切花体内分布发现,2种处理的茎末端、茎上部、花瓣和叶片均有Ag分布,但NS处理的茎末端Ag含量明显高于STS处理,而前者花瓣Ag含量则显著低于后者。
- Abstract:
- Taking cut waxflowers which was highly sensitive to ethylene as material,they were pretreated for 24 h with 10 and 20 mg/L nano-silver (NS) or 0.5 mmol/L silver thiosulfate (STS) solution and using de-ioned (DI) water as control,then immediately placed in vases containing DI water,and exposed to 5 μL/L ethylene for 24 h.The changes in ornamental quality,vase life and flower abscission rate of cut waxflowers during their vase period were investigated.The results showed that exogenous ethylene treatments caused quick wilting and abscission of flowers and obvious inhibition in flower opening of cut waxflowers.Both NS and STS pretreatments could significantly alleviate above harmful effects of exogenous ethylene on cut waxflowers,and the STS treatment was the best and 10 mg/L NS treatment was in second place.Additionally,the distribution of Ag in cut waxflowers after pretreated with 10 mg/L NS and 0.5 mmol/L STS,determined by Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES),demonstrated that Ag could be found in stem end,top of stem,petals and leaves of cut waxflowers pretreated with both STS and NS.However,Ag content in stem end of cut waxflowers pretreated with NS was significantly higher than with STS,while Ag content in their petals of the former was significantly lower than that of the latter.
参考文献/References:
[1] 熊友华,寇亚平.澳大利亚花卉银桦、蜡花的引种栽培研究[J].农业科技与信息,2011(6):15.
[2] Dinh S Q,Joyce D C,Irving D E,et al.Histology of waxflower (Chamelaucium spp.) flower infection by Botrytis cinerea[J].Plant Pathol,2011,60:278-287.
[3] Joyce D C.Postharvest floral organ fall in Geraldton waxflowers (Chamelaucium uncinatum Schauer)[J].Aust J Exp Agr,1993,33:481-487.
[4] Macnish A J,Irving D E,Joyce D C,et al.Sensitivity of Geraldton waxflower to ethylene-induced flower abscission is reduced at low temperature [J].J Horti Sci Biotech,2004,79:293-297.
[5] Joyce D C.Treatments to prevent flower abscission in Geraldton waxflowers [J].Hort Science,1989,24:391.
[6] Beasley D R,Joyce D C.A review of the flower characteristics of Geraldton waxflower and factors influencing their abscission from harvested stems [J].Aust J Exp Agr,2002,42:519-525.
[7] Nell T A.Taking silver safely out of the longevity picture [J].Grower Talks,1992(6):35-42.
[8] Cameron A C,Reid M S 1-MCP blocks ethylene-induced petal abscission of Pelargonium peltatum but the effect is transient [J].Postharvest Biol Technol,2001,22:167-177.
[9] Kim J S,Kuk E,Yu K N,et al.Antimicrobial effects of silver nanoparticles [J].Nanomedicine,2007(3):95-101.
[10] Lok C N,Ho C M,Chen R,et al.Silver nanoparticles:partial oxidation and antibacterial activities [J].J Biol Inorg Chem,2007,12:527-534.
[11] Marambio-Jones C,Hoek E M V.A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment [J].J Nanopart Res,2010,12:1531-1551.
[12] Kim J H,Lee A K,Suh J K.Effect of certain pre-treatment substances on vase life and physiological character in Lilium spp [J].Acta Hort,2005,673:307-314.
[13] 李红梅,林燕飞,刘昌镇,等.纳米银预处理对麝香百合切花的保鲜效应研究[J].北方园艺,2012(8):166-169.
[14] 黄新敏,林启灵,冼锡金,等.纳米银对瓶插月季切花乙烯作用的拮抗效应[J].园艺学报,2012,39(4):735-742.
[15] 刘季平,谢立贤,李红梅,等.不同纳米银处理方式对香石竹切花瓶插寿命的影响[J].仲恺农业工程学院学报,2012,25(4):1-4.
[16] Liu J,Ratnayake K,Joyce D C,et al.Effects of three different nano-silver formulations on cut Acacia holosericea vase life[J].Postharvest Biol Technol,2012,66:8-15.
[17] Choi O,Deng K,Kim N,et al.The inhibitory effects of silver nanoparticles,silver ions,and silver chloride colloids on microbial growth [J].Water Res,2008,42(12):3066-3074.
[18] Asharani P V,Mun G L K,Hande M P,et al.Cytotoxicity and genotoxicity of silver nanoparticles in human cells [J].J Am Chem Soc,2009,3(2):279-290.
[19] Liu J,He S,Zhang Z,et al.Nano-silver pulse treatments inhibit stem end bacteria on cut gerbera cv.Ruikou flowers [J].Postharvest Biol Technol,2009,54:59-62.
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备注/Memo
第一作者简介:刘季平(1982-),女,博士,实验员,研究方向为切花采后生物学。E-mail:liujipingpipi@163.com.
责任作者:何生根(1965-),男,博士,教授,研究方向为观赏植物生物学。E-mail:howtoroot@163.com.
基金项目:国家自然科学基金资助项目(31071829,31272193);
广东省自然科学基金资助项目(10151022501000035,S2012010010418);
广东省高校优秀青年创新人才培育计划资助项目(2012LYM0079)。
收稿日期:2014-01-17