WANG Gui-ping,WANG Jin-zheng,XUE Xiao-min,et al.Effect of Spraying Glycinebetaine on Physiological Responses of Apple Young Trees Under Drought Stress[J].Northern Horticulture,2014,38(12):10-14.
叶面喷施甜菜碱对干旱胁迫下苹果幼树
- Title:
- Effect of Spraying Glycinebetaine on Physiological Responses of Apple Young Trees Under Drought Stress
- 文章编号:
- 1001-0009(2014)12-0010-05
- Keywords:
- glycinebetaine (GB); apple; drought stress; physiological response
- 分类号:
- S 661.1
- 文献标志码:
- A
- 摘要:
- 以1年生“富士”苹果苗(砧木为“平邑甜茶”)为试材,通过盆栽试验,研究了干旱胁迫过程中叶面喷施甜菜碱对苹果幼树叶片中叶绿素、可溶性蛋白质、脯氨酸、可溶性糖、电解质外渗和丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响。结果表明:干旱胁迫过程中,喷施甜菜碱预处理有效缓解了叶绿素和可溶性蛋白质含量的下降,增加了脯氨酸和可溶性糖的含量;改善了苹果的水分状况;抗氧化酶活性也显著增强,膜脂过氧化程度减弱;叶面喷施甜菜碱提高了苹果幼树的抗旱性。
- Abstract:
- Taking one-year-old ‘Fuji’ apple trees as materials(with‘Malus hupehensis’as rootstock),the effect of spraying glycinebetaine on content of chlorophyll,soluble protein content,soluble sugar content,electrolyte leakage rate,MDA content and antioxidant enzyme activity(SOD activity,CAT activity) were studied under drought stress by pot experience.The results showed that exogenous glycinebetaine(GB) alleviated the decreases of chlorophyll and soluble protein,increased the proline content and soluble sugar accumulation in apple leaves during drought stress,ameliorated the water condition;meanwhile,increased the SOD and CAT activities,decreased the MDA content and cell membrane permeability,so the drought resistance of apple young tree was improved.
参考文献/References:
[1] Sakamoto A,Murata N.The role of glycinebetaine in the protection of plants from stress:clues from transgenic plants[J].Plant Cell Environ,2002,25:163-171.
[2] Park E J,Jeknic Z,Sakamoto A,et al.Genetic engineering of glycinebetaine synthesis in tomato protects seeds,plants,and flowers from chilling damage[J].Plant J,2004,40:474-487.
[3] Waditee R,Bhuiyan M N H,Rai V,et al.Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis[J].Proc Natl Acad Sci USA,2005,102:1318-1323.
[4] Ma X L,Wang Y J,Xie S L,et al.Glycinebetaine Application Ameliorates Negative Effects of Drought Stress in Tobacco[J].Russian Journal of Plant Physiology,2007,54(4):472-479.
[5] 马双艳,姜远茂,彭福田,等.水分胁迫对几种果树甜菜碱含量的影响[J].山西果树,2003(5):3-4.
[6] 赵世杰,史国安,董新纯.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002:55-57.
[7] Bajji M,Lutts S,Kinet J M.Water deficit effect on solution contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions[J].Plant Science,2001,160:669-681.
[8] Bartoli C G,Simontacchi M,Tambussi E,et al.Drought and watering dependent oxidative stress:effect on antioxidant content in Triticum aestivum L.leaves[J].J Exp Bot,1999,50:375-383.[9] Terence J,Blake Jiyue L.Hydraulic adjustment in jack pine and black spruce seedlings under controlled cycles of dehydration and rehydration [J].Physiol Plant,2003,117:532-539.
[10] Sudhakar C,Lakshmi A,Giridarakumar S.Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity [J].Plant Sci,2001,161:613-619.
[11] Blum A,Ebercon A.Cell membrane stability as measure of drought and heat tolerance in wheat [J].Crop Sci,1981,21:43-47.
[12] Rehman H,Malik S A,Saleem M.Heat tolerance of upland cotton during fruiting stage evaluated using cellular membrane thermostability [J].Field Crops Res,2004,85:149-158.
[13] 刘艳,王丽雪,王有年.水分胁迫对苹果叶片叶绿体1,6-二磷酸果糖磷酸酯酶活性的影响[J].内蒙古农业大学学报(自然科学版),2002,23(4):42-45.
[14] 罗华建,刘星辉.干旱胁迫对光合特性的影响[J].果树科学,1999,16(2):126-130.
[15] 张建新,徐福利,吕家珑,等.外源甜菜碱对作物的抗旱作用效果研究[J].干早地区农业研究,2003,21(2):86-90.
[16] Anjum S A,Farooq M,Wang L C,et al.Gas exchange and chlorophyll synthesis of maize cultivars are enhanced by exogenously-applied glycinebetaine under drought conditions[J].Plant Soil Environ,2011,57(7):326-331.
[17] Makela P,Kontturi M,Pehu E,et al.Photosynthetic response of drought-and salt-stressed tomato and turnip rape plants to foliar-applied glycinebetaine [J].Physiol Plant,1999,105:45-50.
[18] Ma X L,Wang Y J,Xie S L,et al.Glycinebetaine Application Ameliorates Negative Effects of Drought Stress in Tobacco[J].Russian Journal of Plant Physiology,2007,54(4):472-479.
[19] 邹琦.作物抗旱生理生态研究[M].济南:山东科学技术出版社,1994:4.
[20] 马千全,邹琦,李永华,等.根施甜菜碱对水分胁迫下小麦幼苗水分状况和抗氧化能力的改善作用[J].作物学报,2004(4):321-328.
[21] 张士功,高吉寅,宋景芝.外源甜菜碱对盐胁迫下小麦幼苗体内几种与抗逆能力有关物质含量以及钠钾吸收和运输的影响[J].植物生理学通讯,2000,36(1):23-26.
[22] 刘瑞冬,王有年,王丽雪,等.外源甜菜碱对仁用杏抗旱生理指标的影响[J].内蒙古农业大学学报,2004,25(2):69-72.
[23] 韦建学,李绍鹏,李茂富.外源甜菜碱对香蕉幼苗抗冷性的影响[J].广东农业科学,2007(7):41-43.
[24] Saneoka H,Moghaieb R E A,Premachandra G S,et al.Nitrogen nutrition and water stress effects on cell membrane stability and leaf water relations in Agrostis palustris Huds[J].Environ Exp Bot,2004,52:131-138.
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备注/Memo
第一作者简介:王贵平(1980-),女,山东菏泽人,博士,助理研究员,现主要从事水果育种与栽培生理等研究工作。E-mail:guigui0530@163.com.
基金项目:山东省自然科学基金资助项目(ZR2011CM034)。
收稿日期:2014-03-14