DUAN Guo-feng,WANG Zheng-zheng,LI Li-juan,et al.Effect of High Voltage Electrostatic Field on Physiological Activity of Kidney Bean[J].Northern Horticulture,2014,38(11):24-26.
高压静电场对菜豆生理活性的影响
- Title:
- Effect of High Voltage Electrostatic Field on Physiological Activity of Kidney Bean
- 文章编号:
- 1001-0009(2014)11-0024-03
- 分类号:
- S 643.1
- 文献标志码:
- A
- 摘要:
- 以菜豆品种“翠芸1号”为试材，采用二因素完全随机试验设计，研究了高压静电场场强和处理时间对菜豆种子生理活性的影响。结果表明：300 kV/m、90 min处理游离脯氨酸（Pro）含量最高，为（16.30±1.40）μg/g FW，极显著高于大多数处理及对照；100 kV/m、30 min处理过氧化物酶(POD)活性最高，为（13.02±0.78）μg·g-1·min-1,极显著高于其它处理及对照；200 kV/m、60 min处理超氧化物歧化酶(SOD)活性最高，为（468.36±26.55）U/gFW，极显著高于大多数处理及对照；在每个场强范围内随处理时间的增长其所测指标均呈先上升后下降的变化趋势，100 kV/m、30 min处理下，叶绿素a、叶绿素b和总叶绿素含量以及POD活性与其它处理相比较结果最优。试验表明，适宜的高压静电场处理有利于提高菜豆抗逆性。
- Abstract:
- Taking‘Cuiyun No.1’kidney bean as material,using two factors completely random experiment design,the effect of high voltage electrostatic field and processing time on physiological activity of kidney seed were studied.The results showed that,under the 300 kV/m,90 min treatment,the free proline content was the highest with (16.30±1.40) μg/g FW,significantly higher than most of the processing and control.Under 100 kV/m,30 min treatment,the peroxidase activity was the highest with (13.02±0.78) μg·g-1·min-1,very significantly higher than other treatment and control;under 200 kV/m,60 min processing,the superoxide dismutase SOD activity was the highest,with (468.36±26.55)U/g FW,significantly higher than most of the processing and control;within each electrostatic field as the growth time extended,the measurement indexes presented first rising then decline trend,under 30 min,100 kV/m treatment,chlorophyll a,and chlorophyll b,total chlorophyll content and POD activity were superior to other processing;The experiment showed that suitable high voltage electrostatic field treatment was favour of improving kidney beans resistance.
参考文献/References:
[1] Murr L E.Plant growth response in all electrokencfic field [J].Nature,1963,207(3):1177-1178.
[2] 康敏,余登苑,柳学平,等.静电场对植物生长的生物效应研究[J].农业工程学报,1998,14(4):252-254.
[3] 韩德恩.生物静电效应的利用与开发[J].氨基酸和生物资源,2002,24(3):26-30.
[4] 王愈,李里特.高压电场处理对贮藏番茄活性氧代谢的调节[J].农业工程学报,2009,25(1):255-259.
[5] 高伟娜,顾小清.高压静电场对植物生物学效应的研究进展[J].现代生物医学进展,2006,6（7）:60-62.
[6] 张本华,李成华.高压电场对大豆种子活力影响的试验研究[J].沈阳农业大学学报,2006,37(4):660-662.
[7] 曹永军,习岗,杨初平,等.不同电场对大豆种子萌发的影响[J].应用与环境生物学报,2004,10(6):691-694.
[8] 孙迎春,张羽,李丽雅,等.高压芒刺电场预处理对大豆种子发芽的影响[J].东北师大学报(自然科学版),2005,31(1):54-56.
[9] 蔡兴旺,杨建新.珍珠番茄种子高压静电场处理的田间生物学效应试验[J].种子,2004,23(4):19-22.
[10] 刘志勇,毕玉凤,黄洪云.高压静电场的静电研究[J].唐山师范学院学报,2008,30(2):53-54.
[11] 李合生,孙群,赵世杰,等.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000:136,258-260.
[12] 陈志远,谢菊芳,罗治钢,等.高压静电场预处理对作物种子活力影响的机理探讨[J].湖北大学学报(自然科学版),2003,25(3):224-227.
[13] 张本华,王君玲,陈彬,等.高压静电处理对种子发芽率影响的试验研究[J].农机化研究,2006(5):139-140.
[14] 张永明.高压静电场对黄瓜种子发芽率影响与机理研究[J].长江蔬菜,2008(4):13-16.
[15] 侯福林,金兰芝,柳建军,等.高压静电场对春萝卜种子活力的影响[J].山东农业科学,1995,4(5):32-33.
[16] 王振邦.高压静电场对绿豆种子活力影响的机理探讨[J].陕西教育学院学报,2004,20(2):11-13.
[17] 孙迎春,丁会,徐丽红,等.高压芒刺静电场对玉米种子发芽影响的实验研究[J].东北师大学报(自然科学版),2006,38(1):84-86.
[18] 吴旭红,孙为民,张红燕,等.静电场对植物的生物学效应[J].黑龙江农业科学,2005(2):44-46.
相似文献/References:
[1]南炳东,付金元,肖正璐,等.庆阳市菜豆优良品种引进筛选研究[J].北方园艺,2014,38(12):29.
NAN Bing-dong,FU Jin-yuan,XIAO Zheng-lu,et al.Study on Screening and Introducing of Excellent Phaseolus Vulgaris in Qingyang[J].Northern Horticulture,2014,38(11):29.
[2]于海培,王红宾,王涛.河南省菜豆春季露地品种比较试验[J].北方园艺,2014,38(06):34.
YU Hai-pei,WANG Hong-bin,WANG Tao.Variety Test of Kidney Bean in Spring Open Field in Henan[J].Northern Horticulture,2014,38(11):34.
[3]杨小梅.高寒地区日光温室菜豆引种比较试验[J].北方园艺,2014,38(09):60.
YANG Xiao-mei.Introduction Experiment About Phaseolus vulgaris L.Cultivars in Solar Greenhouse in Alpine Region[J].Northern Horticulture,2014,38(11):60.
[4]张雪,李爽,韩玉珠.利用种子蛋白SDS-PAGE电泳谱带鉴定菜豆品种真实性与纯度的研究[J].北方园艺,2014,38(07):91.
ZHANG Xue,LI Shuang,HAN Yu-zhu.Study on Trueness and Purity Identification of Seeds in Common Bean[J].Northern Horticulture,2014,38(11):91.
[5]王红宾.菜豆新品种“高产架豆王”的选育[J].北方园艺,2013,37(11):182.
WANG Hong-bin.Breeding of A New Kidney Bean Variety- ‘Gaochanjiadouwang’[J].Northern Horticulture,2013,37(11):182.
[6]车培忠,于立芝,于桂香,等.露地栽培菜豆品种比较试验[J].北方园艺,2012,36(02):31.
CHE Pei-zhong,YU Li-zhi,YU Gui-xiang,et al.The Comparative Experiment of Phaseolus vulgaris L. Varieties in Open Field Cultivation[J].Northern Horticulture,2012,36(11):31.
[7]徐 婷,周传余,周 超,等.腐植酸液体叶面肥对保护地菜豆产量与品质的影响[J].北方园艺,2013,37(09):187.
XU Ting,ZHOU Chuan-yu,ZHOU Chao,et al.Effects of Humic Acid Liquid Foliar Fertilizer on Yield and Quality of Greenhouse Beans[J].Northern Horticulture,2013,37(11):187.
[8]田甜,冯国军,盛慧,等.SSR分子标记对菜豆品种鉴定的研究[J].北方园艺,2012,36(04):111.
TIAN Tian,FENG Guo-jun,SHENG Hui,et al.Research of SSR Molecular Markers for Phaseolus vulgaris L.Variety Identification[J].Northern Horticulture,2012,36(11):111.
[9]徐丽鸣,辛 焱,徐 飞.菜豆种质资源色泽性状鉴定与相关性分析[J].北方园艺,2012,36(18):63.
XU Li-ming,XIN Yan,XU Fei.Seed Color Character Identification and Correlation Analysis on Snap Bean Germplasm Resources[J].Northern Horticulture,2012,36(11):63.
[10]张雪梅,宋述尧,张春波,等.塑料大棚栽培菜豆品种筛选研究[J].北方园艺,2014,38(15):49.
ZHANG Xue-mei,SONG Shu-yao,ZHAO Chun-bo,et al.Study on Cultivated Varieties Screening of Snap Bean in Plastic Greenhouse[J].Northern Horticulture,2014,38(11):49.
备注/Memo
第一作者简介：段国锋（1978-)，男，硕士，讲师，研究方向为园艺作物育种及采后生理。E-mail：448052339@qq.com.
基金项目：山西省科技基础平台建设资助项目（201009016）。
收稿日期：2014-03-13