ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Photosynthetic Characteristic and the Content of Chlorophyll and β-carotene in Tomato Seedling Leaves[J].Northern Horticulture,2014,38(11):15-20.
盐胁迫对番茄幼苗叶片光合特性及叶绿素和β-胡萝卜素含量的影响
- Title:
- Effect of Salt Stress on Photosynthetic Characteristic and the Content of Chlorophyll and β-carotene in Tomato Seedling Leaves
- 文章编号:
- 1001-0009(2014)11-0015-06
- 分类号:
- S 641.2
- 文献标志码:
- A
- 摘要:
- 以新疆当地栽培种“石红305”和“黑番茄”为试材，采用基质栽培方法，设置50、100、150、200 mmol/L不同盐胁迫浓度，以0 mmol/L为对照，测定了番茄幼苗叶片的光合特性以及叶绿素a、叶绿素b、总叶绿素和β胡萝卜素的含量变化，以探讨盐胁迫对不同番茄品种光合特性及光合色素含量的影响。结果表明：随盐胁迫程度的增加，2个番茄品种叶片的净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）、胞间CO2浓度（Ci）均表现为先升高后降低的趋势，且光合速率的降低主要由气孔限制因素造成；而水分利用效率（WUE）却基本成增高趋势，这与Pn和Tr的下降幅度有关；叶绿素a含量随盐胁迫浓度的增加而逐渐降低，而叶绿素b、总叶绿素以及β-胡萝卜素含量的变化趋势与净光合速率等的变化趋势相同；这说明一定程度的盐胁迫在影响植物的光合作用、叶绿素合成等初级代谢的同时，也会对其次级代谢色素产生影响，且β-胡萝卜素的含量变化与叶绿素以及净光合速率的变化趋势相符合。
- Abstract:
- Taking two different local tomato cultivars,‘Shihong 305’ and ‘Black tomato’ as materials,under different salt stress level with substrate culture,the effect of 50,100,150,200 mmol/L different salt stress on photosynthetic characteristic and the content of chlorophyll a,chlorophyll b,total chlorophyll and β-carotene in tomato seedling leaves were studied,in order to explore the salt stress on the influence of photosynthetic characteristic and content of photosynthetic pigment.The results showed that,the general tends of Pn, Tr, Gs and Ci in the two cultivars’s leaves were all first increased then decreased together with the rise of salt content in different treatment,and the main cause of Pn decreasement was stomatal limitation.But the WUE was higher in heavy salt stress treatment,this most related to the descent of Pn and Tr. The content of chlorophyll a was decreased along with the change of salt content,but the change of chlorophyll b,total chlorophyll and β-carotene were same to Pn.This illustrated that salt stress could affect the photosynthesis and chlorophyll synthesis in plant leaves and also had influence on the content of secondary pigments,further more,the change of β-carotene content was correlated to chlorophyll content and Pn in tomato seedling leaves.
参考文献/References:
[1] 加孜拉?阿山,王修贵,姚宛艳,等.新疆土壤盐碱化治理技术初步研究[J].节水灌溉,2011(11):50-52.
[2] 朱新广,张其德.NaCl对光合作用影响的研究进展[J].植物学通报,1999,16(4):332-338.
[3] 孙璐,周宇飞,李丰先,等.盐胁迫对高粱幼苗光合作用和荧光特性的影响[J].中国农业科学,2012,45(16):3265-3272.
[4] 鲁少尉,齐飞,李天来,等.NaCl胁迫对番茄叶片光合特性及蔗糖代谢的影响[J].北方园艺,2012(9):14-18.
[5] 武维华,张蜀秋.袁明,等.植物生理学[M].北京:科学出版社,2003:122-124.
[6] Télef N,Stammitti-Bert L,Mortain-Bertrand A,et al.Sucrose deficiency delays lycopene Accumulation in tomato fruit pericarpdiscs[J].Plant Mol Biol,2006,62:453-469.
[7] 何强强,惠伯棣,宫平,等.类胡萝卜素代谢物的生物学活性研究进展[J].食品科学,2011,32(15):289-295.
[8] Bhimanagouda S P,Jayaprakasha G K.Tropical and subtropical fruits:flavors,color,and health benefits[M].American Chemical Society,2013:43-61.
[9] 牛艳,许兴,魏玉清,等.不同产地土壤因子与宁夏枸杞中β-胡萝卜素关系的研究[J].农业科学研究,2005,26(2):21-39.
[10] 中国无土栽培技术论坛.常用无土栽培营养液配方[EB/OL].http://www.soilles.com.2012-11-07.
[11] 刘蕾.新疆土壤盐胁迫的组成和分布特征[J].干旱环境监测,2009,23(4):227-229.
[12] 张蜀秋,李云.植物生理学试验技术教程[M].北京:科学出版社,2011:70-72.
[13] 刘国顺,韦凤杰,王芳,等.反相高效液相色谱法测定烤烟叶片发育过程中的类胡萝卜素类物质[J].色谱,2006,24(2):161-163.
[14] 王新超,陈亮,赵丽萍,等.反相高效液相色谱法测定茶叶中β-胡萝卜素含量[J].中国农学通报,2006,22(9):91-93.
[15] Sudhir P R,Murthy S D S.Effects of salt stress on basic processes of photosynthesis[J].Photosynthetica,2004,42(4):481-486.
[16] Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33:317-345.
[17] 陈淑芳,朱月林,刘友良,等.NaCl胁迫对番茄嫁接苗保护酶活性、渗透调节物质含量及光合特性的影响[J].园艺学报,2005,32(4):609-613.
[18] 王素平,李娟,郭世荣,等.NaCl胁迫对黄瓜幼苗植株生长和光合特性的影响[J].西北植物学报,2006,26(3):455-461.
[19] 曹生奎,冯起,司建华,等.植物叶片水分利用效率研究综述[J].生态学报,2009,29(7):3382-3892.
[20] 王素平,郭世荣,李璟,等.盐胁迫对黄瓜幼苗根系生长和水分利用的影响[J].应用生态学报,2006,17(10):1883-1888.
[21] 李汉美,何勇.NaCl对番茄嫁接苗光合作用和叶绿素荧光特性的影响[J].西北农业学报,2013,22(3):131-134.
[22] 张纪涛,徐猛,韩坤,等.盐胁迫对番茄幼苗的营养及生理效应[J].西北农业学报,2011,20(2):128-133.
[23] 束胜,郭世荣,孙锦,等.盐胁迫下植物光合作用的研究进展[J].中国蔬菜,2012(18):53-61.
[24] 康燕玉,谢文玲,高亚辉,等.不同浓度NaCl和光照强度对杜氏藻体内β-胡萝卜素含量的影响[J].植物生理学通讯,2006,42(2):315-318.
相似文献/References:
[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]石秋梅,邓翻云,吴敏言,等.罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J].北方园艺,2014,38(12):128.
SHI Qiu-mei,DENG Fan-yun,WU Min-yan,et al. Study on Salt Tolerance of Apocynum venetum Linn.and Poacynum hendersonii(Hook.f.) Woodson at Stages of Seed Germination and Seedlings Growth[J].Northern Horticulture,2014,38(11):128.
[3]常青山,张利霞,郑轶琦,等.盐胁迫下钙离子对苜蓿种子萌发的影响[J].北方园艺,2014,38(10):64.
CHANG Qing-shan,ZHANG Li-xia,ZHENG Yi-qi,et al.Effects of Ca2+?on Seed Germination Characteristics of Medicago sativa Under Salt Stress[J].Northern Horticulture,2014,38(11):64.
[4]杨 凯,孙迎坤,谭 雯,等.盐胁迫对香石竹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(22):86.
YANG Kai,SUN Ying-kun,TAN Wen,et al.Effects of Salt Stress on Seeds Germination and Seedling Growth of Carnation[J].Northern Horticulture,2013,37(11):86.
[5]张 娇,张大治,马 艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014,38(14):65.
ZHANG Jiao,ZHANG Da-zhi,MA Yan,et al.Effect of NaCl Simulation of Salt Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus[J].Northern Horticulture,2014,38(11):65.
[6]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(11):1.
[7]何会流.外源水杨酸对盐胁迫下凤仙花种子萌发特性的影响[J].北方园艺,2013,37(13):75.
HE Hui-liu.Effect of Exogenous Salicylic Acid on the Seeds Germination Characters of Impatiens balsamina L.Under NaCl Stress[J].Northern Horticulture,2013,37(11):75.
[8]刘 杰.盐碱胁迫对向日葵体内矿质营养的影响[J].北方园艺,2014,38(02):1.
LIU Jie.Effects of Simulated Salt and Alkali Conditions on the Mineral Nutrition of Helianthus annuusL.[J].Northern Horticulture,2014,38(11):1.
[9]张芬,张波,田丽萍,等.盐胁迫对加工番茄叶片番茄红素[J].北方园艺,2014,38(04):82.
ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Expression of Lycopene Cyclase Gene in Processing Tomato Leaves[J].Northern Horticulture,2014,38(11):82.
[10]刘新星,罗俊杰.外源钙对盐胁迫下豌豆幼苗生化指标的影响[J].北方园艺,2013,37(14):24.
LIU Xin-xing,LUO Jun-jie.Effects of Exogenous Calcium on Physiological Indexes of Pea Seedlings Under Salt Stress[J].Northern Horticulture,2013,37(11):24.
备注/Memo
第一作者简介：张芬（1988-），女，硕士研究生，研究方向为植物营养生理生态。E-mail：zhangfen2008.cool@163.com.
责任作者：田丽萍（1961-），女，硕士，教授，研究方向为植物营养生理生态和生药学。E-mail：lipingt@163.com.
基金项目：国家“十二五”农村领域科技计划资助项目［2011BAD35B07(02)］。
收稿日期：2014-03-13