GUO Chun-hui,SHA Wei,LI Xiao-kai.Effect of Drought Stress on Physiological Characteristics of Three Moss Species[J].Northern Horticulture,2014,38(09):78-82.
干旱胁迫对三种藓类植物生理特性的影响
- Title:
- Effect of Drought Stress on Physiological Characteristics of Three Moss Species
- 文章编号:
- 1001-0009（2014）09-0078-05
- Keywords:
- silica gel drought stress; Racomitrium japonicum; Grimmia pilifera; Tortula ruralis; physiological characteristics
- 分类号:
- Q 945.78
- 文献标志码:
- A
- 摘要:
- 以东亚砂藓、毛尖紫萼藓和山墙藓3种藓类植物为试材，选取不同的时间梯度，对其进行3个月的硅胶干燥处理，研究了干旱胁迫对3种藓类植物相对水分含量（RWC）、丙二醛(MDA)含量、过氧化物酶(POD)活性、游离脯氨酸（Pro）含量、可溶性蛋白质（SP）含量、可溶性糖（SS）含量等6项生理指标的影响。结果表明：经硅胶干旱胁迫处理的3种藓类，随着相对水分含量的逐渐降低，3种藓类植物体内的丙二醛含量均呈现先降低后升高再下降再升高的趋势；山墙藓POD活性较另外2种藓类低，且变化不明显，而东亚砂藓和毛尖紫萼藓在不同时期POD活性有较大变化；3种藓类的渗透调节物中，游离脯氨酸含量变化与可溶性糖含量变化呈反相关，可溶性蛋白质含量变化与POD活性呈正相关；6种生理指标的变化从一定程度上反映出3种藓类具有不同于其它植物的特殊抗旱生理机制。
- Abstract:
- Taking three kinds of mosses including Racomitrium japonicum Dozy & Molk,Grimmia pilifera P.Beauv.and Tortula ruralis Gaertn as materials,three kinds of mosses were subjected to drought treatment by silica gel for three months of different time gradients,and the effect of drought stress on six physiological indexes of them including relative water content (RWC),malondialdehyde (MDA) content,POD activity,free proline (Pro) content,soluble protein (SP) content and soluble sugar (SS) content were studied.The results showed that the malondialdehyde content in the three mosses treated with silica drought stress was gradually decreased and then increased and reveals the same trend once again along with the relative water content reduced.POD activity of Tortula ruralis was lower than those of the other two mosses,and changes inconspicuously,while the POD activity of Racomitrium japonicum and Grimmia pilifera in different periods showed great changes.Among osmoregulation substances of the three moss species,the change of free proline content and soluble sugar (SS) content were inversely related,however,change of soluble protein (SP) content and POD activity was positively related.To some extent,the six physiological indexes indicated that the three mosses may have some special physiological mechanisms those were different from other plants.
参考文献/References:
[1]关周博,郑磊,李殿荣,等.干旱半干旱地区甘蓝型油菜产量与相关经济性状的灰色关联度分析[J].陕西农业科学,2012(6):19-22. [2]蒲伟凤,纪展波,李桂兰,等.作物抗旱性鉴定方法研究进展(综述)[J].河北科技师范学院学报,2011(2):34-39. [3]Yong R Q,Yan C N,Jiang B H,et al.The comparison of contents of malondia ldehyde and proline in the area of Karst area in northweastern Guangxi Province[J].Agricultural Science Technology,2011,12(4):469473,575. [4]丁玉梅,马龙海,周晓罡,等.干旱胁迫下马铃薯叶片脯氨酸、丙二醛含量变化及与耐旱性的相关性分析[J].西南农业学报,2013,26(1):106-110. [5]史玉炜,燕凌,李文兵,等.水分胁迫对刚毛柽柳可溶性蛋白、可溶性糖和脯氨酸含量变化的影响[J].新疆农业大学学报,2007,30(2):5-8. [6]杨保义,张杰.变色硅胶干燥剂吸湿性能研究[J].装备环境工程,2010,7(2):32-35. [7]Shu C M,Xiu Q L,Lian P M,et al.Comparison of methods for ultradrying of Chinese chive seeds[J].Agricultural Science and Technology,2013,14(4):598-601. [8]上海植生所.现代植物生理实验指南[M].北京:中国科技出版社,1998:128-145. [9]李忠光.愈创木酚法测定植物过氧化物酶活性的改进[J].植物生理学通讯,2008,44(2):323-324. [10]高峻风.植物生理学实验技术[M].西安:世界图书出版社,2000:11-26. [11]张志良,瞿伟菁,李小方.植物生理学实验指导[M].北京:高等教育出版社,2009. [12]张蕾.生物化学实验指导[M].武汉:武汉大学出版社,2011. [13]刘奉觉,郑世锴,臧道群.杨树几个水分关系指标的主分量分析[J].植物生理学报,1986(3):13-16. [14]Gholz H L,Ewel K C,Teskey R O.Water and forest productivity[J].Forest Ecology and Management,1990,30:1-18. [15]张慧,汪沛洪.叶片相对含水量的活体测定[J].植物生理学通讯,1991,27(3):17-19. [16]周智彬,徐新文,杨兰英.3种固沙植物对高温胁迫的生理响应及其抗热性研究[J].干旱区地理,2005,28(6):824-830. [17]冉飞,包苏科,石丽娜,等.干旱胁迫和复水对锡金微孔草抗氧化酶系统的影响(简报)[J].草业学报,2008,17(5):156-160. [18]白娟,龚春梅,王刚.干旱胁迫下荒漠植物红砂叶片抗氧化特性[J].西北植物学报,2010,30(12):2444-2450. [19]魏娟,谢福春,王华田,等.水分胁迫对海州常山抗逆生理特性的影响[J].山东农业大学学报(自然科学版),2009,40(3):371-376. [20]潘东明,郑国华,徐开生,等.水分胁迫对枇杷SOD、POD及脂质过氧化的影响[C]//园艺学论文集.北京:北京农业大学出版社,1995:149-154. [21]刘世鹏,刘济明,陈宗礼,等.模拟干旱胁迫对枣树幼苗的抗氧化系统和渗透调的影响[J].西北植物学报,2006,26(9):1781-1787. [22]李晓清,曹小军,王启和,等.希蒙得木幼苗对干旱胁迫的响应[J].水土保持学报,2010,24(5):268-273. [23]康俊梅,杨青川,樊奋成.干旱对苜蓿叶片可溶性蛋白的影响[J].草地学报,2005,13(3):199-202. [24]吉增宝,王进鑫.干旱胁迫对侧柏幼树某些生理特性的影响[J].西北林学院学报,2009,24(6):6-9. [25]钱永生,王慧中.渗透调节物质在作物干旱逆境中的作用[J].杭州师范学院学报(自然科学版),2006,5(6):476-481.
备注/Memo
第一作者简介：国春晖（1988-），男，硕士研究生，研究方向为植物生理生化。E-mail：343061426@qq.com.