HAI Xiao-xia,LV Fei,WANG Zhi-gang,et al.Determination of Semilethal Temperature of Three Varieties of Weigela florida Under Low Temperature Stress and Evaluation on Their Cold Resistance[J].Northern Horticulture,2014,38(07):56-61.
三个锦带花栽培品种低温半致死温度的测定及其抗寒性分析
- Title:
- Determination of Semilethal Temperature of Three Varieties of Weigela florida Under Low Temperature Stress and Evaluation on Their Cold Resistance
- 文章编号:
- 1001-0009（2014）07-0056-06
- Keywords:
- Weigela florida; relative electric conductivity; relative K+ leakage; free water; bound water; semilethal temperature(LT50)
- 分类号:
- S 687
- 文献标志码:
- A
- 摘要:
- 以锦带花属的红王子锦带、花叶锦带和四季锦带3个优良的锦带花栽培品种当年生枝条为试材，通过人工冰冻方法，测定了模拟低温0（CK）、-10、-20、-30、-40℃条件下3个锦带花品种离体枝条的K+相对渗出率和相对电导率，同时利用Logistic方程拟合K+相对渗出率及相对电导率与人工模拟低温变化关系，推算出3个品种的低温半致死温度(LT50)；并对3个品种离体枝条的自由水含量、束缚水含量以及二者比值的变化进行了比较。结果表明：在模拟低温条件下，3个品种离体枝条的K+相对渗出率和相对电导率均随温度降低逐渐增大，且总体上与CK有显著差异；对K+相对渗出率和相对电导率与温度进行Logistic方程拟合推算出花叶锦带、红王子锦带、四季锦带的半致死温度分别为-35.60、-32.21、-31.50℃和-34.88、-31.14、-28.44℃；随着胁迫温度的降低，3个品种的束缚水含量均高于自由水含量，其中束缚水和自由水的比值排序为花叶锦带＞红王子锦带＞四季锦带。综合比较，3个锦带花品种抗寒性强弱顺序为花叶锦带＞红王子锦带＞四季锦带。
- Abstract:
- Taking the current twigs which of three varieties of Weigela florida (Weigela florida cv. Red Prince,Weigela florida cv.Variegata, Weigela florida(Bunge) A.DC) as materials，by artificial freezing method and atomic absorption spectrometer,the relative K+leakage and electric conductivity of dormant,the current twigs which of three varieties of Weigela florida were calculated with different low temperature 0 (CK),-10,-20,-30,-40℃.Regression models were established between low temperature (T) and the relative K+leakage and electric conductivity to calculate the semilethal temperatures (LT50)；and measured the free and bound water and its ratio of the three different varieties of stem tissue.The results showed that the relative K+leakage and conductivity of dormant the current twigs gradually increased with the decreasing temperature under low temperature stress,it showed significant difference compared with the control（P＜0.05）；the semi-lethal temperatures (LT50) of three varieties of Weigela florida were calculated respectively by logistic equation regression analysis of the relative K+ leakage and eletric conductivity,which of Weigela florida cv Red Prince,Weigela florida cv.Variegata,Weigela florida(Bunge) A.DC were -35.60,-32.21,-31.50℃ and -34.88,-31.14,-28.44℃.The bound water content of three varieties were higher than the free water under low temperature stress.The order of bound water/free water ratio was Weigela florida cv.Variegata > Weigela florida cv. Red Prince>Weigela florida(Bunge) A.DC.The comprehensive comparison results showed that the cold resistance of the three varieties was in the order of Weigela florida cv.Variegata >Weigela florida cv. Red Prince> Weigela florida(Bunge) A.DC.
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备注/Memo
第一作者简介：海小霞（1984-），女，山西怀仁人，硕士研究生，研究方向为城市林业。E-mail：moqi.hai@foxmail.com.