XU Lu-jia,HUA Yun-fen.Research Advances on the Tissue Culture of Protocorm of Dendrobium[J].Northern Horticulture,2014,38(06):191-195.
石斛原球茎的组织培养研究进展
- Title:
- Research Advances on the Tissue Culture of Protocorm of Dendrobium
- 文章编号:
- 1001-0009(2014)06-0191-05
- Keywords:
- Dendrobium; protocorm; tissue culture; research advance
- 分类号:
- R 931.71
- 文献标志码:
- A
- 摘要:
- 石斛是名贵的中草药之一，含有多糖、生物碱、氨基酸和微量元素等多种成分，具有增强免疫、抗肿瘤、抗疲劳、降血糖和养阴生津等多种生物活性。现对影响石斛原球茎增殖和分化的培养基、植物生长调节剂、金属元素与附加物、碳源和多胺、培养方式等因素以及原球茎的保存和有效成分积累进行了综述；总结了石斛原球茎多糖的生物活性作用；并对今后的研究重点进行了展望，建议应该在寻找影响石斛原球茎生长速度和生物产量的各种理化因素上进行深入研究。
- Abstract:
- Dendrobium is one of the valuable chinese herbal medicine.It contains polysaccharides,alkaloids,amino acids,trace elements and other ingredients,which have many biological activities,such as strengthening immunity,anti-tumor,anti-fatigue,hypoglycemic and regenerating body fluid.Currently affecting protocorm proliferation and differentiation medium,plant growth regulators,metallic elements and appendages,carbon and polyamines,training methods and other factors and the preservation and accumulation of the active ingredients of the protocorm were reviewed；the biological activity polysaccharide of protocorms were summarized.And future research priorities were discussed,the proposal should be looking at a variety of physical and chemical factors affecting protocorm growth rate and biomass yield in-depth study.
参考文献/References:
[1]刘莉,萧凤回.石斛属药用植物多糖研究进展[J].现代中药研究与实践,2009,23(1):77. [2]郑泉,郭维明,郑勇平,等.超声波辅助诱导春石斛原球茎途径再生体系的建立[J].江西农业大学学报,2009,31(6):1019. [3]莫昭展,贝学军.铁皮石斛原球茎增殖条件的研究[J].福建林业科技,2007,34(4):55-58. [4]莫昭展,贝学军,符韵林,等.流苏石斛原球茎增殖研究[J].林业科技,2008,33(6):59. [5]魏小勇.铁皮石斛种胚原球茎液体悬浮培养研究[J].中国现代应用药学,2005,22(6):471-473. [6]张桂芳,关杰敏,黄松,等.铁皮石斛原球茎的诱导与增殖影响因素研究[J].中药材,2011,34(8):1172. [7]杨柳平,刘畅庆,赵仁发,等.铁皮石斛种子诱导原球茎组培快繁体系的研究[J].广东农业科学,2012,39(7):54-57. [8]戴传云,刘腾飞,管天冰,等.铁皮石斛原球茎增殖培养基配方的优化[J].安徽农业科学,2011,39(10):5788-5789. [9]莫昭展,贝学军,韦江萍,等.不同培养条件对铁皮石斛原球茎增殖的影响[J].安徽农业科学,2007,35(22):6835-6836. [10]林丛发,钟爱清,林云斌,等.铁皮石斛类原球茎增殖和分化的研究[J].江西农业学报,2007,19(1):84-86. [11]沐德俊,高剑英.霍山石斛原球茎在不同培养方式下生长状态的研究[J].上海农业科技,2011(3):19-21. [12]魏小勇.铁皮石斛原球茎悬浮培养研究[J].现代中药研究与实践,2004,18(4):7-11. [13]侯丕勇,郭顺星.悬浮培养的铁皮石斛原球茎在固体培养基上生长和分化的研究[J].中国中药杂志,2005,30(10):729-732. [14]尹明华,聂凤琴,胡文韬,等.NAA和KT对铁皮石斛原球茎增殖与分化的影响[J].黑龙江农业科学,2011(8):25. [15]邵世光,侯北伟,周琪,等.基于正交实验法的铁皮石斛原球茎分化和生根条件研究[J].南京师大学报,2009,32(4):98. [16]蒋波,杨存亮,黄捷,等.铁皮石斛原球茎生长分化及生根壮苗研究[J].玉林师范学院学报,2005,26(3):66-69. [17]包英华,白音,何清清.美花石斛原球茎增殖及分化的研究[J].安徽农业科学,2007,35(3):675-677. [18]Luo J P,Christoph W,Brigitte K.Enhanced micropropagation of Dendrobium huoshanense C Z Tang et S J Cheng through protocorm-like bodies：The effects of cytokinins,carbohydrate sourcesand cold pretreatment[J].Scientia Horticulturae,2009,123:258-262. [19]Panisa P,Ronnawich S,Pongsathorn C.Improving the micropropagation efficiency of hybrid Dendrobium orchids with chitosan [J].Scientia Horticulturae,2010,124:490-499. [20]Wang Y,Luo J P,Wu H Q.Conversion of protocorm-like bodies of dendrobium huoshanense to shoots:The role of polyaminesin relation to the ratio of total cytokinins and indole-3-acetic acidindole-3-acetic acid[J].Journal of Plant Physiology,2009,166:2013-2022. [21]Wei M,Wei S H,Yang C Y.Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots[J].Plant Cell Tiss Organ Cult,2010,102:145-151. [22]Lin Y,Li J,Li B.Effects of light quality on growth and developmentof protocorm-like bodies of Dendrobium officinale in vitro[J].Plant Cell Tiss Organ Cult,2011,105:329-335. [23]Wei M,Yang C Y,Wei S H.Enhancement of the differentiation of protocorm-like bodies of Dendrobium officinale to shoots by ultrasound treatment [J].Journal of Plant Physiology,2012,169:770-774. [24]王君晖,张毅翔,刘峰等.铁皮石斛种子、原球茎和类原球茎体的超低温保存研究[J].园艺学报,1999,26(1):59-61. [25]蒙爱东,余丽莹,董青松,等.铁皮石斛原球茎常温保存研究[J].广西植物,2009,29(6):808. [26]涂红艳,刘伟,叶庆生.细茎石斛拟原球茎生长与有效成分积累的关系[J].生物磁学,2004,4(3):37-41. [27]黄蓓,洪萨丽,金青,等.硝普钠、植酸和水杨酸对悬浮培养的霍山石斛原球茎生长和生理活性的影响[J].植物生理学通讯,2010,46(5):423. [28]陈晓梅,孟志霞,郭顺星.真菌诱导子与铁皮石斛原球茎作用条件的优化[J].中国药学杂志,2008,43(20):1545-1549. [29]姚睿,朴炫春,李铁军,等.利用气升式生物反应器培养铁皮石斛原球茎[J].中国中药杂志,2012,37(24):3763-3767. [30]苏江,罗兴录,何铁光,等.铁皮石斛原球茎固体培养和液体悬浮培养动力学研究[J].西北农业学报,2007,16(4):161-165. [31]韦晓新,苏江,何铁光.几种因素对铁皮石斛原球茎生长和多糖积累的影响[J].广西农业科学,2008,39(5):601. [32]何铁光,杨丽涛,李杨瑞,等.蔗糖对铁皮石斛原球茎生长与多糖积累的影响[J].安徽农业科学,2007,35(13):3817-3819. [33]苏江,岑忠用,何铁光.中途添加不同浓度蔗糖对铁皮石斛原球茎多糖积累的影响[J].广东农业科学,2010,37(9):65. [34]宋经元,郭顺星,肖培根.氮源和真菌诱导子对铁皮石斛原球茎悬浮培养的影响[J].云南植物研究,2008,30(1):105-109. [35]查学强,罗建平,姜绍通.悬浮培养霍山石斛原球茎合成活性多糖的研究[J].食品科学,2005,26(4):41-44. [36]苏江,岑忠用,邓晰朝.基于正交试验法的铁皮石斛原球茎生长和多糖合成研究[J].作物杂志,2013(2):84-90. [37]王增利,史昊,张宗申.铁皮石斛原球茎悬浮培养及其多糖积累的研究[J].河南农业科学,2012,41(2):129-131. [38]何铁光,杨丽涛,李杨瑞,等.铁皮石斛原球茎多糖DCPP1a-1对氧自由基和脂质过氧化的影响[J].天然产物研究与开发,2007,19(3):410-414. [39]何铁光,杨丽涛,李杨瑞,等.铁皮石斛原球茎多糖粗品与纯品的体外抗氧活性研究[J].中成药,2007,29(9):1265-1269. [40]何铁光,杨丽涛,李杨瑞,等.铁皮石斛原球茎多糖DCPP1a-1的理化性质及抗肿瘤活性[J].天然产物研究与开发,2007,19(4):578-583. [41]李胜立,陈程,杨思林,等.霍山石斛类原球茎免疫调节活性的有效部位及其毒理安全性评价[J].药物评价研究,2012,35(5):321-327. [42]林宏,施松善,王顺春.铁皮石斛原球茎多糖D-02C的结构特征及其抗补体活性[J].福建中医药,2012,43(3):54-57. [43]Zha X Q,Luo J P,Jiang S T.Enhancement of polysaccharide production in suspension cultures ofprotocorm-like bodies from Dendrobium huoshanense by optimizationof medium compositions and feeding of sucrose[J].Process Biochemistry,2007,42:344-351. [44]陈程,吴胡琦,查学强,等.霍山石斛组织快繁中不同发育阶段多糖理化性质和免疫活性的比较研究[J].中药材,2012,35(8):1195-1199.
相似文献/References:
[1]张文龙.石斛种质资源遗传多样性的PAGE分析[J].北方园艺,2014,38(14):104.
ZHANG Wen-long.PAGE Analysis of Genetic Diversity of Dendrobium Germplasm Resources[J].Northern Horticulture,2014,38(06):104.
[2]黄作喜,张 杨,颜小玉,等.铁皮石斛原球茎高效增殖体系的构建[J].北方园艺,2014,38(14):107.
HUANG Zuo-xi,ZHANG Yang,YAN Xiao-yu,et al.Establishment for a Highly Efficient Proliferation System of Protocorm of Dendrobium officinale Kimura et Migo[J].Northern Horticulture,2014,38(06):107.
[3]董海丽,刘红.湿法超微粉碎提取石斛多糖的研究[J].北方园艺,2013,37(15):150.
DONG Hai-li,LIU Hong.Study on Extraction of Dendrobium candidumPolysaccharide by Wet Ultrafine Grinding Technology[J].Northern Horticulture,2013,37(06):150.
[4]李 翠,张占江,韦 莹,等.药用植物马鞭石斛组织培养与快速繁殖[J].北方园艺,2013,37(06):105.
LI Cui,ZHANG Zhan-jiang,WEI Ying,et al.Study on Tissue Culture and Rapid Propagation of Dendrobium fimbriatum Hook.var. oculatum.Hook[J].Northern Horticulture,2013,37(06):105.
[5]龚建英,王华新,孙利娜,等.九种引种石斛生物学特性及栽培适应性研究[J].北方园艺,2013,37(20):75.
GONG Jian-ying,WANG Hua-xin,SUN Li-na,et al.Study on Biological Characteristics and Cultivation Adaption of Nine Introductional Dendrobium [J].Northern Horticulture,2013,37(06):75.
[6]韩晓红,罗明英,欧阳志成,等.金线莲原球茎增殖研究[J].北方园艺,2012,36(24):144.
HAN Xiao-hong,LUO Ming-ying,OUYANG Zhi-cheng,et al.Study on the Proliferation of Protocorm-like Bodies in Anoectochilus roburghii[J].Northern Horticulture,2012,36(06):144.
[7]徐萌,郭绍霞,孙丽,等.大花蕙兰原球茎诱导、增殖与分化影响因素研究[J].北方园艺,2016,40(16):108.[doi:10.11937/bfyy.201616028]
XU Meng,GUO Shaoxia,SUN Li,et al.Influence Factors on Induction，Proliferation and Differentiation of Protocorm of Cymbidium hybridum[J].Northern Horticulture,2016,40(06):108.[doi:10.11937/bfyy.201616028]
[8]吴彦秋,吕享,李小兰,等.杜鹃兰原球茎增殖培养条件[J].北方园艺,2016,40(19):124.[doi:10.11937/bfyy.201619031]
WU Yanqiu,LYU Xiang,LI Xiaolan,et al.Culture Conditions of Protocorms Proliferation of Cremastra appendiculata[J].Northern Horticulture,2016,40(06):124.[doi:10.11937/bfyy.201619031]
[9]李东徽,关文灵,古 今,等.云南石斛农业生产环节SOP规程构建的探讨[J].北方园艺,2011,35(03):0.[doi:10.11937/bfyy.201103072]
LI Dong-hui,GUAN Wen-ling,GU Jin,et al.Discussion on the Construction of SOP of Agricultural Production of Dendrobium sp.[J].Northern Horticulture,2011,35(06):0.[doi:10.11937/bfyy.201103072]
[10]包英华,白 音,任安祥,等.生物技术在我国石斛产业上的应用[J].北方园艺,2007,31(04):0.[doi:10.11937/bfyy.200704034]
[J].Northern Horticulture,2007,31(06):0.[doi:10.11937/bfyy.200704034]
备注/Memo
第一作者简介：徐路加（1990-），男，硕士研究生，研究方向为天然产物化学。E-mail:657608499@qq.com.