LU Shun-jiao,YI Shuang-shuang,LIU Guo-feng,et al.Research on Pollen Germination and Pollen Preservation of Platanus acerifolia[J].Northern Horticulture,2014,38(06):62-65.
二球悬铃木花粉萌发及花粉保存研究
- Title:
- Research on Pollen Germination and Pollen Preservation of Platanus acerifolia
- 文章编号:
- 1001-0009（2014）06-0062-04
- 分类号:
- S 792
- 文献标志码:
- A
- 摘要:
- 以新鲜采集的二球悬铃木花粉为试材，研究了不同浓度的蔗糖及硼酸对花粉萌发的影响，并在此基础上研究了不同贮藏温度对干燥与未干燥的二球悬铃木花粉生活力的影响。结果表明：在15%蔗糖+0.10%硼酸的液体培养基上花粉萌发最好；不同贮藏温度对花粉的生活力影响显著，随着贮藏温度的降低，花粉生活力上升，且贮藏时间延长，常温条件下贮藏10 d后花粉生活力为0%，而在-72℃条件下贮藏38 d，干燥花粉仍有24.65%生活力；花粉经硅胶干燥4 h后耐贮性明显高于新鲜花粉。综合分析，经硅胶干燥后于-72℃条件下贮藏利于二球悬铃木花粉的保存。
- Abstract:
- Taking pollen of Platanus acerifolia as material,the effect of different sucrose and boric acid concentration on the pollen germination of Platanus acerifolia was studied,and then the effect of different storage temperature on pollen viability was analyzed.The results showed that pollen had the highest germination rate at the liquid medium with 15% sucrose and 0.10% boric acid.Storage temperature had a significant effect on pollen viability,with the reduction of storage temperature,the pollen viability risen and extended storage time.At normal temperature,all detected pollen loss viability after 10 days,but it had 24.65% viability when storage at -72℃ after 38 days.Moreover,the storage property of dry pollen was higher than fresh pollen.In conclusion,dry pollen and storage at -72℃ was favourable for preservation of Platanus acerifolia pollen.
参考文献/References:
[1]Besnard G,Tagmount A,Baradat P,et al.Molecular approach of genetic affinities between wild and ornamental Platanus[J].Euphytica,2002,126:401-412. [2]陈有民.园林树木学(园林专业用)[M].北京:中国林业出版社,1990:400-403. [3]李志能,刘国锋,罗春丽,等.悬铃木花粉生活力及贮藏力的研究[J].武汉植物学研究,2006,24(1):54-57. [4]赵兴华,张道旭,印东生,等.百合花粉生活力测定和贮藏方法研究[J].北方园艺,2009(2):172-175. [5]胡君艳,李云,孙宇涵,等.银杏花粉生活力测定及贮藏方法的优化[J].中国农学通报,2008,24(5):148-153. [6]冯岚,洑香香,方升佐.青钱柳花粉生活力及耐贮性的研究[J].北京林业大学学报,2010(4):121-125. [7]何莉,张华珍,贾文庆.广玉兰花粉生活力测定及贮藏方法研究[J].江苏农业科学,2013(4):164-166. [8]何莉,贾文庆.郁李花粉生活力及贮藏特性的研究[J].天津农业科学,2013(5):5-8. [9]马杰,李桂荣,张玉园,等.不同培养条件对红碧桃花粉生活力的影响[J].资源开发与市场,2013,29(5):457-460. [10]张铭芳,吴磊磊,贾桂霞.百合不同杂交系品种花粉贮藏特性分析[J].西北植物学报,2013,33(7):1465-1472. [11]吴子龙,赵昕,牛红雷,等.不同干燥法与贮藏温度对玉兰花粉萌发的影响[J].北方园艺,2013(1):59-61. [12]盖树鹏,盖伟玲,郑国生.不同条件下牡丹花粉的贮藏寿命[J].北方园艺,2011(18):104-106. [13]黄春球,宋天顺,李明静,等.白芨花粉活力测定与花粉保存[J].北方园艺,2011(3):182-184. [14]刘自刚.桔梗花粉贮藏研究[J].北方园艺,2009(4):73-75. [15]王小敏,吴文龙,李维林,等.黑莓花粉含水量与贮藏特性的研究[J].江西农业学报,2013,25(5):35-37. [16]孟令威,毕晓颖,郑洋.紫花鸢尾花粉萌发及贮藏性研究[J].中国农学通报,2012(28):192-197.
相似文献/References:
[1]李 荣.紫荆花粉生活力测定及贮藏方法研究[J].北方园艺,2014,38(11):116.
LI Rong.Study on the Determination of Cercis chinensis Pollen Viability and Its Storage Method[J].Northern Horticulture,2014,38(06):116.
[2]覃屏生,张 瑜,侯丽霞,等.沙田柚花柱蛋白对其花粉萌发及生长的影响[J].北方园艺,2014,38(03):88.
QIN Ping-sheng,ZHANG Yu,HOU Li-xia,et al.Effect of Citrus grandis var.shatinyu Hort.Style Protein on Its Pollen Germination and Growth[J].Northern Horticulture,2014,38(06):88.
[3]吴子龙,赵昕,牛红雷,等.不同干燥法与贮藏温度对玉兰花粉萌发的影响[J].北方园艺,2013,37(01):59.
WU Zi-long,ZHAO Xin,NIU Hong-lei,et al.Effect of Different Drying Method and Storage Temperature on the Pollen Germination of Yulania denudata[J].Northern Horticulture,2013,37(06):59.
[4]刘雪莲,秦佳梅,孙珺博.培养基组分对锦带花粉离体萌发的影响[J].北方园艺,2012,36(24):129.
LIU Xue-lian,QIN Jia-mei,SUN Jun-bo.Effect of Medium Components on Pollen Germination in vitro of Diervilla sessilifolia[J].Northern Horticulture,2012,36(06):129.
[5]韩雪平,薛晓敏,王金政,等.‘凯特’杏花粉离体培养条件筛选[J].北方园艺,2015,39(03):96.[doi:10.11937/bfyy.201503029]
HAN Xue-ping,XUE Xiao-min,WANG Jin-zheng,et al.Screening Factors That Impact Pollen Growth of Prunus armeniaca L.cv ‘Katy’ in vitro[J].Northern Horticulture,2015,39(06):96.[doi:10.11937/bfyy.201503029]
[6]李琛,窦玉娟,刘丽叶,等.甜樱桃花粉原位萌发荧光显微镜观察[J].北方园艺,2018,42(05):54.[doi:10.11937/bfyy.20173289]
LI Chen,DOU Yujuan,LIU Liye,et al.Fluorescence Microscope Observation on Pollinated Styles of Sweet Cherry[J].Northern Horticulture,2018,42(06):54.[doi:10.11937/bfyy.20173289]
[7]杨芩,陈磊,张婷渟,等.花龄对‘灿烂’兔眼蓝莓花粉原位萌发和花粉管生长的影响[J].北方园艺,2018,42(09):57.[doi:10.11937/bfyy.20172906]
YANG Qin,CHEN Lei,ZHANG Tingting,et al.Effects of Flower Age on Pollen Germination and Pollen Tube Growth of ‘Britewell’ Rabbitteye Blueberry[J].Northern Horticulture,2018,42(06):57.[doi:10.11937/bfyy.20172906]
备注/Memo
第一作者简介：陆顺教（1981-），男，博士，助理研究员，现主要从事园林花卉遗传育种与生物技术等研究工作。E-mail：lushunjiao@sina.com.