[1]王亚军,安巍,罗青,等.丛枝菌根真菌菌剂扩繁及菌根化枸杞育苗技术研究[J].北方园艺,2014,38(05):139-143.
Research on Enlargment Reproducing of AMF Microbial Inoculum and Growing Seedling Technology of Wolfberry With Mycorrhization.WANG Ya-jun1,2,AN Wei1，LUO Qing1，MA Ping3,SHI Zhi-gang1，ZHAO Jian-hua1[J].Northern Horticulture,2014,38(05):139-143.
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Research on Enlargment Reproducing of AMF Microbial Inoculum and Growing Seedling Technology of Wolfberry With Mycorrhization.WANG Ya-jun1,2,AN Wei1，LUO Qing1，MA Ping3,SHI Zhi-gang1，ZHAO Jian-hua1[J].Northern Horticulture,2014,38(05):139-143.
丛枝菌根真菌菌剂扩繁及菌根化枸杞育苗技术研究
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
卷:
第38卷
期数:
2014年05期
页码:
139-143
栏目:
出版日期:
2014-03-15
- Title:
- WANG Ya-jun1,2,AN Wei1，LUO Qing1，MA Ping3,SHI Zhi-gang1，ZHAO Jian-hua1
- 文章编号:
- 1001-0009(2014)05-0139-05
- Keywords:
- Ambuscular Mycorrhiza Fungus(AMF); enlargement reproducing; mycorrhization; wolfberry; growing seedling technology
- 分类号:
- R 33
- 文献标志码:
- A
- 摘要:
- 以“宁杞7号”枸杞为试材，以红三叶草和玉米2种宿主植物对4种丛枝菌根真菌（AMF）菌种进行了菌剂扩繁，采用枸杞嫩枝扦插育苗技术，利用自繁的AMF菌剂进行枸杞菌根化苗木繁育；苗木出圃后田间定植，调查田间的性状表现，以期为AMF在枸杞上的应用和深层研究提供了理论参考。结果表明：采用红三叶草和玉米2种植物作为宿主植物可以扩繁出高质量的丛枝菌根真菌菌剂，侵染率在94%以上，基质中孢子密度168～351个/50g干土；接种后育苗成活率均在60%以上，各处理间差异不大；接种处理的幼苗根系中均含有大量的真菌的菌丝体，菌根真菌的侵染率达43%以上；菌根化苗木的苗高生长明显高于对照，其中缩球囊霉、根内球囊霉和混合接种处理分别较对照提高了53.45%～89.66%，达极显著差异水平，摩西球囊霉接种处理较对照提高了39.66%，达显著差异水平；接菌处理的地径的生长量虽高于对照，但多重比较分析表明，不存在差异的显著性。
- Abstract:
- Taking wolfberry ‘Ningqi No.7’ as tested material,4 Ambuscular Mycorrhiza Fungus(AMF) microbial inoculum had been enlarged reproduced with Trifolium pretense L.and Zae mays.With self-reproduced microbial inoculum,wolfberry seedling with mycorrhization had been reared with microbial inoculum using young-shoot-cuttage seedling technology of wolfberry.After removed from the garden，wolfberry seedling with mycorrhization were planted in test field and the field performance were investigated.It could provide theoretical reference for the application of AMF on the wolfberry and deep research.The results showed that the high quality AMF microbial inoculum could been reproduced using Trifolium pretense L.and Zae mays as host plants.The mycorrhizal fungi colonization rate was more than 94%.The spores density was from 168 to 351 per 50 g dried substrate.The survival rate of wolfberry seedling was more than 60% after inoculated by AMF microbial inoculum,and had little difference among various treatments.There were lots of fungal myceliums in the roots of wolfberry seedling inoculated by AMF microbial inoculum.The mycorrhizal fungi colonization rate of wolfberry seedling was more than 43%.The seedling height growth of wolfberry seedling with mycorrhization obviously higher than the control，and the treatment with Glomus constrictum Trappe, Glomus intraradices Schenck & Smith and mixture had extreme difference with control（P<0.01）,increased by 53.45% to 89.66% respectively.The treatment with Glomus mosseae(Nicolson & Gerdemann had striking difference with the control（P<0.05）,increased by 39.66% than that of control.The basal diameter growth higher than the control,but the LSD multi-comparative analysis showed that there no striking difference to the control.The research can offer reference for applications and deeper research of AMF on wolfberry.
参考文献/References:
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备注/Memo
第一作者简介：王亚军（1979-），男，甘肃定西人，硕士，助理研究员，现主要从事枸杞育种及枸杞种质资源等研究工作。E-mail：yajun817@163.com.责任作者：安巍（1970-），男，宁夏中宁人，副研究员，现主要从事枸杞育种及枸杞种质资源等研究工作。E-mail:angouqi@163.com.基金项目：宁夏回族自治区科技攻关计划资助项目（2011ZYH088）；宁夏自然科学基金资助项目（NZ1179)。
更新日期/Last Update:
2014-08-10