DAI Min,SUN Kun,SU Xue,et al.Effect of Pb on Seed Germination and Seedling Growth of Arctium lappa [J].Northern Horticulture,2014,38(04):132-135.
铅胁迫对牛蒡种子萌发及幼苗生长的影响
- Title:
- Effect of Pb on Seed Germination and Seedling Growth of Arctium lappa
- 文章编号:
- 1001-0009(2014)04-0132-05
- Keywords:
- Arctium lappa; Pb; seed germination; seedling growth
- 分类号:
- R 282.71
- 文献标志码:
- A
- 摘要:
- 以牛蒡为试材，研究浓度为0、50、150、300、600、800、1 000 mg/L的铅（Pb）溶液对牛蒡种子萌发和幼苗生长的影响。结果表明：Pb溶液浓度为150 mg/L时发芽率最高，50、150 mg/L低浓度Pb溶液对牛蒡种子的萌发具有促进效应，当Pb溶液浓度大于150 mg/L时则表现出递增抑制效应；不同浓度Pb胁迫下，牛蒡种子的发芽势、发芽指数和活力指数以及幼苗鲜重均表现出先升高后降低的趋势，且除幼苗鲜重外均在Pb2+浓度150 mg/L时出现峰值；幼苗根长和芽长在Pb2+不同浓度胁迫下同样呈现出先增加后减小的趋势，其中幼苗根长和芽长分别在Pb2+浓度为50、150 mg/L时出现峰值；幼苗叶片丙二醛和叶绿素含量随Pb2+浓度增加表现出持续升高趋势；幼苗叶片中超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性均表现出随Pb2+浓度增加先升高后降低的趋势，但二者活性在较高浓度Pb胁迫（>600 mg/L）时仍能保持较高水平。试验结果表明，低浓度Pb处理对牛蒡种子萌发和幼苗生长具有一定促进效应，高浓度Pb则对其产生抑制作用，但高浓度Pb胁迫下牛蒡种子仍具有较高的萌发率，幼苗也可以正常生长，SOD、POD保持较高活性。
- Abstract:
- Taking Arctium lappaas material，the effect of different Pb solution concentrations on seed germination and seedling growth of Arctium lappawere studied.The results showed that the seed germination rate was the highest at the 150 mg/L Pb2+ solution concentration；Pb stimulated the seed germination at 50 mg/L and 150 mg/L concentrations，and restrained the seed germination at the higher concentrations (>150 mg/L).All the seed germination potential，germination index，and vital index，as well as the seedling fresh weight showed out low-high-low tendency，and the crest value were at the 150 mg/L Pb2+solution concentration except the seedling fresh weight.The root and bud lengths of seedlings showed out the same tendency as the seedling fresh weight，but the crest values were at the 50 mg/L and 150 mg/L Pb2+solution concentration respectively.The malondialdehyde (MDA) content and chlorophyll content of seedling leaves increased with the Pb2+solution concentrations.The activities of SOD and POD also showed out low-high-low tendency，but they remained high levels under conditions of high Pb2+solution concentrations (>600 mg/L).The results indicated that Pb stimulated the seed germination and seedling growth ofArctium lappaat the lower concentrations，and restrained the seed germination at the higher concentrations，but Arctium lappa seed still had higher germination rate and seedling could also be normal growth at the high concentrations.SOD activity and POD activity under high solution concentration of Pb stress still had a highly reactive.Through its antioxidant system against adversity，Arctium lappa?had a certain resistance to Pb stress.
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备注/Memo
第一作者简介：戴敏（1987-），男，硕士研究生，研究方向为植物生理学。E-mail：daimin8864@163.com.