QIAN Zong-wei,CHEN Hai-li,CUI Yan-ling.Rapid Hybrid Purity Identification of Eggplant by SSR Markers[J].Northern Horticulture,2014,38(23):75-78.
茄子种子纯度SSR标记快速鉴定
- Title:
- Rapid Hybrid Purity Identification of Eggplant by SSR Markers
- 文章编号:
- 1001-0009（2014）23-0075-04
- Keywords:
- eggplant; SSR molecular marker; purity identification
- 分类号:
- S 641.1
- 文献标志码:
- A
- 摘要:
- 以茄子杂交种“京茄218”及其父母本为试材，采用SSR分子标记技术，研究了其双亲及F1之间的多态性，并结合田间鉴定对其进行验证。结果表明：在152对SSR引物中有2条引物（qzssr17和qzssr20）的扩增产物在杂交种中呈现父母本互补带型。利用qzssr17引物对6个制种单位繁制的“京茄218”杂交种进行SSR纯度鉴定，其鉴定结果与田间鉴定结果具有较好的一致性。试验证明，与田间种植鉴定相比，SSR标记是一种更准确、简单高效的茄子种子纯度鉴定方法。
- Abstract:
- Taking the new eggplant hybrid “Jingqie 218” and their parents as experimental materials，the polymorphisms of the materials were analyzed and sifted by using SSR molecular markers，and the results were verified by morphological identification.The results showed that among the 152 pairs of SSR primers，2 pairs of primers (qzssr17 and qzssr20) showed complementary pattern between hybrids and their parents.The hybrids “Jingqie 218” which were produced by 6 units had been indentified by using the qzssr17 SSR molecular markers，the results had better consistency with the morphological identification results.The experiment proved that using SSR molecular markers was a simpler and effective method to identify the purity of eggplant.
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备注/Memo
第一作者简介：潜宗伟(1982-)，男，硕士，助理研究员，研究方向为蔬菜遗传育种。E-mail：qianzongwei@nercv.org.