WANG Gang,ZHOU Rong,WANG Ping,et al.Characterization of Stress-tolerance on NaCl of Two Kinds of Tomato Explants[J].Northern Horticulture,2014,38(21):123-126.
番茄两种外植体对氯化钠胁迫耐性的研究
- Title:
- Characterization of Stress-tolerance on NaCl of Two Kinds of Tomato Explants
- 文章编号:
- 1001-0009(2014)21-0123-04
- Keywords:
- tomato; variety; explant; NaCl stress
- 分类号:
- S641.203.8
- 文献标志码:
- A
- 摘要:
- 以番茄下胚轴和子叶为外植体，在培养基中添加不同浓度的NaCl，研究了番茄2个品种、2种外植体在愈伤组织和不定芽诱导过程中对NaCl胁迫的耐性。结果表明：随着NaCl胁迫浓度的增加抑制愈伤组织和不定芽的产生，形成愈伤组织和不定芽所需要的时间延长，番茄2个品种、2种外植体在NaCl浓度为100～125 mmol/L时愈伤组织诱导率显著地低于对照，NaCl浓度在50～75 mmol/L时不定芽诱导率显著地低于对照，不定芽形成比愈伤组织形成过程对NaCl的耐性差。
- Abstract:
- Using hypocotyl and cotyledon as explants, stress-tolerance on NaCl of the 2 kinds of explants in 2 tomato varieties were investigated in the process of callus and adventitious bud induction by adding different concentration of NaCl in the media. The results indicated that induction of callus and appearance of adventitious bud would be suppressed with the increase of concentration of NaCl stress. Time needed for the formation of callus and adventitious bud would also be extended. When the stress of NaCl reached to 100-125 mmol/L, rate of callus induction from 2 tomato varieties, as well as 2 kinds of explants were observed to be lower significantly than those in control. Under the stress of 50-75 mmol/L of NaCl, induction rates of adventitious buds showed to be significantly lower than those in control.Adventitious bud formation was affected more sensitive than callus formation under the NaCl stress.
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备注/Memo
第一作者简介：王罡（1964-），男，博士，教授，现主要从事植物基因工程与分子生物学研究工作。E-mail：wanggangtjdx@126.com.
基金项目：国家自然科学基金资助项目（31271419）。
收稿日期：2014-07-14