ZHANG Yun-feng,HU Fen.ISSR Analysis of Phenotypic Variants in Tissue Culturing Regeneration Plants of Anthurium andraeanum[J].Northern Horticulture,2014,38(21):101-104.
红掌组培再生植株中表型突变体的ISSR分析
- Title:
- ISSR Analysis of Phenotypic Variants in Tissue Culturing Regeneration Plants of Anthurium andraeanum
- 文章编号:
- 1001-0009（2014）21-0101-04
- Keywords:
- Anthodium andraeanum; ISSR; mutagenesis; tissue culturie
- 分类号:
- S 682.1+4;Q 943.1
- 文献标志码:
- A
- 摘要:
- 以红掌组培再生植株中发生表型突变的植株为试材，采用PCR多态性分析的方法，研究组织培养再生植株中产生的表型变异株的基因变化。结果表明：利用筛选的8条ISSR引物进行PCR多态性分析，共获得73条扩增产物，其中多态性条带22条，多态性程度为30.2%。聚类树状图表明，“亚利桑娜”品种及其组织培养再生植株的遗传距离在0.0274~0.3636之间，它们之间具有非常相似的遗传背景。其中变异株V1与亲本相比，在基因型上的变化不显著，而变异株V2、V3、V4的基因型与亲本显著不同，有些条带消失，并产生了一些新的条带；在组织培养再生植株中产生的表型变异株可能是组织培养过程中通过遗传重组产生的新基因型，它们将为花卉育种提供一种新的种质资源来源。
- Abstract:
- Taking the phenotypic variants of Anthodium andraeanum as material,using method of PCR,the gene changes of phenotypic variants in tissue culture was researched.The results showed that total 73 ISSR markers were acquired with 8 polymorphic ISSR primers which were screened from the 37 ISSR primers,and averagely 9.1 products for each ISSR primer.22 polymorphic ISSR markers (taking 30.2%) were observed during the research,showing that the genetic changes certainly existed between the tested plants.The genetic distance was 0.0274-0.3636,showing their similar genetic background between them.The clusters analyses showed with UPGMA (unweighted pair group method with arithmetic averages) method revealed that V1 had little genotype changes comparing to there parent cultivar Arizona,but V2,V3 and V4 exhibited significant genotype changes.The results showed that new genotype could be generated as new genetic recombination occurs in the tissue culturing.
参考文献/References:
[1]Isabelle R S,Philippe L,Lionel G,et al Specific detection of xanthomonas axonopodis pv.dieffenbachiae in Anthurium (Anthurm andreanum) tissues by nested PCR[J].Appl Environ Microbiol,2006,72(2)：1072-1078.
[2]Ahloowalia B S.Regeneration of ryegrass plants in tissue culture[J].Crop Sci,1975,15：449-452.
[3]Yesodi V,Izhar S,Hauschner H,et al.Homologous recombination involving cox2 is responsible for a mutation in the CMS-specific mitochondrial locus of Petunia[J].Molecular General Genetic,1997,255:106-114.
[4]熊丹,谢伟,陈发菊,等.香果树组织培养过程中遗传变异的RAPD分析[J].植物生理学通讯,2008,1(48):37-41. [5]胡鑫,徐全乐.连续组织培养引起大岩桐再生植株的表型变化[J].西北农业学报,2010,19(5):167-170.
[6]Tang W.In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets[J].Plant Cell Reports,2001,20:163-168.
[7]Martins M,Sarmento D,Oliveira M M.Genetic stability of microp-ropagated almond plantlets,as assessed by RAPD and ISSR markers[J].Plant Cell Reports,2004,23:492-496.
[8]Valladares S,Sánchez C,Martínez M T,et al.Plant regeneration through somatic embryogenesis from tissues of mature oak trees:true-to-type conformity of plantlets by RAPD analysis[J].Plant Cell Reports,2005,23:492-496.
[9]Amzad H M,Konisho K,Minami M,et al.Somaclonal variation of regenerated plants in chili pepper(Capsicum annuum L.)[J].Euphytica,2003,130:233-239.
[10]王桂兰,陈超,李朝霞,等.红掌气生根根段再生快繁体系的建立[J].植物生理学通讯,2005,41(3):297-301.
[11]范永山,刘海英,李卫国,等.利用CTAB 法提取红掌不同部位基因组DNA[J].河北农业大学学报,2006,29(3):36-38.
[12]张志胜,黎扬辉,姜蕾,等.红掌四倍体的离体诱导及其鉴定[J].园艺学报,2007,34(3):729-734.
[13]Jain S M.Tissue culture-derived variation in crop improvement[J].Euphytica,2001,118:153-166.
[14]缪恒彬,陈发棣,赵宏波,等.应用ISSR 对25个小菊品种进行遗传多样性分析及指纹图谱构建[J].中国农业科学,2008,41(11):3735-3740.
[15]吴振兴,王慧中,施农农,等.兰属Cymbidium植物ISSR遗传多样性分析[J].遗传,2008,30(5):627-632.
[16]谷守芹,范永山,李坡,等.玉米大斑病菌ISSR反应体系的优化和遗传多样性分析[J].植物保护学报,2008(5):427-432.
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备注/Memo
第一作者简介：张运峰（1982-），男，硕士研究生，实验师，研究方向为植物分子生物学。E-mail:yunfengzhang1982@126.com.
责任作者：胡芬(1983-)，女，博士，讲师，研究方向为生物化学与分子生物学。E-mail:ihufen@126.com.
基金项目：国家自然科学基金资助项目（81302323）；河北省自然科学基金资助项目(C2014209140)。
收稿日期：2014-05-27