WANG Bo,MA Xing,LIU Ying-ying,et al.Research Advance of NRT Gene on Nitrogen Use Efficiency[J].Northern Horticulture,2014,38(20):198-202.
NRT基因与氮素利用效率的研究进展
- Title:
- Research Advance of NRT Gene on Nitrogen Use Efficiency
- 文章编号:
- 1001-0009(2014)20-0198-05
- 分类号:
- Q 78
- 文献标志码:
- A
- 摘要:
- 低氮素利用效率对农田生态系统所造成的污染日益加重,如何提高作物的氮素利用效率已成为目前的一个研究热点。文章分析了植物体内硝酸盐转运体(Nitrate transporters,NRT)的分类、存在部位、生物学功能及调控机制,旨在阐明植物吸收、转运NO3-的生理机制,指出了利用分子生物学的手段是提高作物氮素利用效率的一个重要方向,这为今后通过基因工程的手段来提高氮素利用效率,减少环境污染提供了一定的理论依据。
- Abstract:
- Lower nitrogen use efficiency(NUE)caused the aggravation of the ecosystem pollution.How to improve the NUE has become a hot research goal.To clarify the physiological characteristics of absorption and transportation of NO3- by plants,the classification of the NRT and its presence organ,biology functions and regulatory mechanisms in plant was summarized in this paper and pointed out.There was an important way to improve the NUE of plant by the means of molecular biology.A theoretical basis for the future to improve NUE and reduce environmental pollution by methods of genetic engineering were provided.
参考文献/References:
[1]赵其国.土壤科学发展的战略思考[J].土壤,2009,41(5):681-688.
[2]赵其国,骆永明,滕应.中国土壤保护宏观战略思考[J].土壤学报,2009,46:1140-1145.
[3]Yan X,Jin J Y,He P,et al.Recent advances on the technologies to increase fertilizer use efficiency[J].Agricultural Science in China,2008,7(4):469-479.
[4]蔡昉.中国“三农”政策的60年经验与教训[J].广东社会科学,2009,(6):148-157.
[5]陆景陵,胡霭堂.植物营养学[M].北京:高等教育出版社,2006.
[6]Robertson G P,Vitousek P M.Nitrogen in agriculture:balancing the cost of an essential resource[J].Annual Review Environment Resources,2009,34:97-125.
[7]周健民,石元亮.面向农业与环境的土壤科学综述篇[M].沈阳:科学出版社,2004.
[8]齐景发.中国农业统计资料[M].北京:中国农业出版社,1998.
[9]曹仁林,贾晓葵.我国集约化农业中氮污染问题及防治对策[J].土壤肥料,2001(3):3-6.
[10]李庆逵,朱兆良,于天仁.中国农业持续发展中的肥料问题[M].南昌:江西科学技术出版社,1998:3-5.
[11]Dobermann A.Nitrogen use efficiency-state of the art.In:IFA international workshop on enhanced-efficiency fertilizers[M].Frankfurt,Germany,2005:28-30.
[12]闫湘,金继运,何萍,等.提高肥料利用率技术研究进展[J].中国农业科学,2008,41(2):450-459.
[13]张福锁,王激清,张卫峰,等.我国主要粮食作物肥料利用率现状与提高途径[J].土壤学报,2008,45(5):915-924.
[14]Coque M,Gallais A.Genomic regions involved in response to grain yield selection at high and low nitrogen fertilization in maize[J].Theoretical and Applied Genetics,2006,112:1205-1220.
[15]Chardon F,Barthélémy J,Daniel-Vedele F,et al.Natural variation of nitrate uptake and nitrogen use efficiency in Arabidopsis thaliana cultivated with limiting and ample nitrogen supply[J].Journal of Experimental Botany,2010,61:2293-2302.
[16]Masclaux-Daubresse C,Chardon F.Exploring nitrogen remobilization for seed filling using natural variation in Arabidopsis thaliana[J].Journal of Experimental Botany,2011,62:2131-2142.
[17]朱兆良.我国氮肥的使用现状、问题和对策[M]//李庆逵,朱兆良,于天仁.中国农业持续发展中的肥料问题.南京:江苏科学技术出版社,1998:38-51.
[18]Ju X T,Kou C L,Zhang F S,et al.Nitrogen balance and groundwater nitrate contamination:Comparison among three intensive cropping systems on the North China Plain[J].Environmental Pollution,2006,143(1):117-125.
[19]Liu X J,Ju X T,Zhang Y,et al.Nitrogen deposition in agroecosystems in the Beijing area[J].Agriculture,Ecosystems and Environment,2006,113:370-377.
[20]Food and Agriculture Organization.FAO statistical databases,agriculture date[EB/OL].http://faostat.fao.org/faostat.2005.
[21]王波,赖涛,孙兴祥,等.增铵营养对生菜根系生长发育影响的研究[J].植物营养与肥料学报,2006,12(5):745-749.
[22]Zhang H,Forde B G.An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture[J].Science,1998,279:407-409.
[23]Xu G H,Fan X R,Anthony J M.Plant nitrogen assimilation and use efficiency[J].Annual Review of Plant Biology,2012,63:153-182.
[24]Bi Y M,Kant S,Clark J,et al.Increased nitrogen-use efficiency in transgenic rice plants over-expressing a nitrogen-responsive early nodulin gene identified from rice expression profiling[J].Plant Cell and Environment,2009,32:1749-1760.
[25]Wang Y Y,Hsu P K,Tsay Y F.Uptake,allocation and signaling of nitrate[J].Trends in Plant Science,2012,17(8):458-467.
[26]Feng H,Yan M,Fan X,et al.Spatial expression and regulation of rice high-affinity nitrate transporters by nitrogen and carbon status[J].Journal of Experimental Botany,2011,62:2319-2332.
[27]Lam H M,Coschigano K,Oliveira I C,et al.The molecular-genetics of nitrogen assimilation into amino acids in higher plants[J].Annual Review of Plant Physiology and Plant Molecular Biology,1996,47:569-593.
[28]Gojon A,Krouk G,Perrine W F,et al.Nitrate transceptor(s)in plants[J].Journal of Experimental Botany,2011,62:2299-2308.
[29]Dechorgnat J,Nguyen C T,Armengaud P,et al.From the soil to the seeds:the long journey of nitrate in plants[J].Journal of Experimental Botany,2011,62:1349-1359.
[30]Liu K H,Huang C Y,Tsay Y F.CHL1 is a dual-affinity nitrate transporter of Arabidopsis involved in multiple phases of nitrate uptake[J].The Plant Cell,1999,11(5):865-874.
[31]Ho C H,Lin S H,Hu H C,et al.CHL1 functions as a nitrate sensor in plants[J].Cell,2009,138:1184-1194.
[32]Huang N C,Liu K H,Lo H J,et al.Cloning and functional characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake[J].The Plant Cell,1999,11(8):1381-1392.
[33]Okamoto M,Vidmar J J,Glass A D M.Regulation of NRT 1 and NRT 2 gene families of Arabidopsis thaliana:Responses to nitrate provision[J].Plant and Cell Physiology,2003,44(3):304-317.
[34]Chiu C C,Lin C S,Hsia A P,et al.Mutation of a nitrate transporter,AtNRT 1∶4,results in a reduced petiole nitrate content and altered leaf development[J].Plant and Cell Physiology,2004,45(9):1139-1148. [35]Almagro A,Lin S H,Tsay Y F.Characterization of the Arabidopsis nitrate transporter NRT 1.6 reveals a role of nitrate in early embryo development[J].Plant Cell,2008,20(12):3289-3299.
[36]Fan S C,Lin C S,Hsu P,et al.The Arabidopsis nitrate transporter NRT 1.7,expressed in phloem,is responsible for source-to-sink remobilization of nitrate[J].The Plant Cell,2009,21(9):2750-2761.
[37]Weichert A,Brinkmann C,Komarova N,et al.AtPTR 4 and AtPTR 6 are differentially expressed,tonoplast-localized members of the peptide transporter/nitrate transporter 1(PTR/NRT1)family[J].Planta,2012,235:311-323.
[38]Dietrich D,Hammes U,Thor K.AtPTR 1,a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis[J].The PlantJournal,2004,40:488-499.
[39]Song W,Koh S,Czako M,et al.Antisense express ion of the peptide transport gene AtPTR2-B delays flowering and arrests seed development in transgenic Arabidopsis plants[J].Plant Physiology,1997,114(3):927-935.
[40]Komarova N Y,Thor K,Gubler A,et al.AtPTR1 and AtPTR5 transport dipeptides in planta[J].Plant Physiology,2008,148:856-869.
[41]Plett D,Toubia J,Garnett T,et al.Dichotomy in the NRT gene families of dicots and grass species[J].PLoS ONE,2010,5:e15289.
[42]Yan M,Fan X R,Feng H M,et al.Rice OsNAR 2.1 interacts with OsNRT 2.1,OsNRT 2.2 and OsNRT 2.3a nitrate transporters to provide uptake over high and low concentration ranges[J].The Plant Cell and Environment,2011,34:1360-1372.
[43]Orsel M,Filleur S,Fraisier V,et al.Nitrate transport in plants:which gene and which control?[J].Journal of Experimental Botany,2002,53:825-833.
[44]Kiba T,Feria-Bourrellier A B,Lafouge F,et al.The Arabidopsis nitrate transporter NRT 2.4 plays a double role in roots and shoots of nitrogen-starved plants[J].Plant Cell,2012,24(1):245-258.
[45]Chopin F,Orsel M,Dorbe M F,et al.The Arabidopsis ATNRT 2.7 nitrate transporter controls nitrate content in seeds[J].The Plant Cell,2007,19:1590-1602.
[46]Little D Y,Rao H,Oliva S,et al.The putative high-affinity nitrate transporter NRT 2.1 represses lateral root initiation in response to nutritional cues[J].Procedins of the National Academy of Science of the United States of America,2005,102(38):13693-13698.
[47]Wang R,Okamoto M,Xing X,et al.Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1,000 rapidly responding genes and new linkages to glucose,trehalose-6-phosphate,iron,and sulfate metabolism[J].Plant Physiology,2003,132:556-567.
[48]Okamoto M,Kumar A,Li W,et al.High-affinity nitrate transport in roots of Arabidopsis depends on expression of the NAR 2-Like gene AtNRT 3.1[J].Plant Physiology,2006,140(3):1036-1046.
[49]Krouk G,Lacombe B,Bielach A,et al.Nitrate-regulated auxin transport by NRT 1.1 defines a mechanism for nutrient sensing in plants[J].Development Cell,2010,18(6):927-937.
[50]Garnett T,Conn V,Kaiser B N.Root based approaches to improving nitrogen use efficiency in plants[J].Plant Cell and Environment,2009,32:1272-1283.
[51]Filleur S,Daniel-Vedele F.Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display[J].Planta,1999,207(3):461-469.
[52]Cerezo M,Tillard P,Filleur S,et al.Major alterations of the regulation of root NO3- uptake are associated with the mutation of Nrt 2.1 and Nrt 2.2 genes in Arabidopsis[J].Plant Physiology,2001,127:262-271.
[53]Okamoto M,Vidmar J J,Glass A D M.Regulation of NRT 1 and NRT 2 gene families of Arabidopsis thaliana:responses to nitrate provision[J].Plant and Cell Physiology,2003,44(3):304-317.
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备注/Memo
第一作者简介:王波(1965-),男,博士,副教授,硕士生导师,现主要从事农业资源与利用等教学与科研工作。E-mail:wangb@suda.edu.cn.
基金项目:苏州市科技局资助项目(SYN201313)。
收稿日期:2014-06-10