HUANG Feng-xiang,GUAN Yan,GUI Ming-chun,et al.Study on the Callus from the Anther of Hevea brasiliensis Mull.Arg by Orthogonal Experiment[J].Northern Horticulture,2014,38(18):109-111.
正交实验法优选橡胶树花药愈伤组织诱导的研究
- Title:
- Study on the Callus from the Anther of Hevea brasiliensis Mull.Arg by Orthogonal Experiment
- 文章编号:
- 1001-0009（2014）18-0109-03
- Keywords:
- orthogonal experiment; Hevea brasiliensis; anther; callus
- 分类号:
- S 794.1
- 文献标志码:
- A
- 摘要:
- 以橡胶树品种RRII105花药为外植体，研究了不同浓度2,4-D、KT、NAA及TDZ组合对橡胶树花药愈伤组织诱导的影响。结果表明：最适合诱导橡胶树花药愈伤组织的培养基配方为MS+0.8 mg/L 2，4-D+0.8 mg/L KT+1.2 mg/L NAA+0.0 mg/L TDZ+60 g/L蔗糖+5 g/L琼脂，诱导率达94.00%，出胚率为14.25%。
- Abstract:
- Taking anther of Hevea brasiliensis clone RRII105 as explants,the influence of different concentrations of plant growth regulators(2,4-D,KT,NAA,TDZ) on callus induction of anther were studied.The results showed that the best medium was MS+0.8 mg/L 2，4-D+0.8 mg/L KT+1.2 mg/L NAA+0.0 mg/L TDZ+60 g/L sucrose+5 g/L agar.The inductive rate of callus was 94.00%，the inductive rate of embryoid was 14.25%.
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备注/Memo
第一作者简介：黄凤翔（1962-），女，农艺师，现主要从事植物组织培养等研究工作。E-mail:782205527@qq.com.
基金项目：国家科技支撑计划资助项目（2011BAD30B01）；云南省产业重大关键技术开发专项计划资助项目（云发改[2008]1657号）；云南省应用基础研究自筹经费资助项目（2013FZ169）；2014云南省热带作物科技创新体系建设资金资助项目（RF2014）;云南省科研院所技术开发研究专项资助项目（2013DC013）。
收稿日期：2014-04-21