[1]林群英,吴亮亮,张锋伦,等.蛹虫草栽培过程中胞外蛋白酶活性变化研究[J].北方园艺,2014,38(17):148-150.
　LIN Qun-ying,WU Liang-liang,ZHANG Feng-lu,et al.Study on Extracellular Protease Activity Change During Fruitbody Growth of Cordyceps militaris[J].Northern Horticulture,2014,38(17):148-150.
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蛹虫草栽培过程中胞外蛋白酶活性变化研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年17期 页码: 148-150 栏目: 出版日期: 2014-09-15
Title:
Study on Extracellular Protease Activity Change During Fruitbody Growth of Cordyceps militaris
文章编号:
1001-0009(2014)17-0148-03
作者:
林群英1; 2; 吴亮亮1; 2; 张锋伦1; 吴素玲1; 孙晓明1; 3; 项春荣3
1.南京野生植物综合利用研究院，江苏 南京 210042；
2.江苏鸿丰果蔬食品有限公司，江苏 宿迁 223700；
3.镇江市丹徒区正东生态农业发展中心，江苏 镇江 212000
Author(s):
LIN Qun-ying1; 2; WU Liang-liang1; 2; ZHANG Feng-lu1; WU Su-ling1; SUN Xiao-ming1; 3; XIANG Chun-rong3
1.Nanjing Institute for the Comprehensive Utilization of Wild Plants,Nanjing,Jiangsu 210042；
2.Jiangsu Hongfeng Fruit and Vegetable Co.Ltd.,Suqian,Jiangsu 223700;
3.Zhengdong Ecological Agriculture Development Center of Zhenjiang City Dantu District,Zhenjiang,Jiangsu 212000
关键词:
蛹虫草; 子实体栽培; 胞外蛋白酶
Keywords:
Cordyceps militaris; fruiting body cultivation; extracellular proteases
分类号:
S567.35
文献标志码:
A
摘要:
以蛹虫草为试材，研究蛹虫草生长过程中子实体鲜重、培养基pH值、胞外酸性蛋白酶、中性蛋白酶和碱性蛋白酶的活性变化。结果表明：蛹虫草酸性蛋白酶的活性大小受氮源的影响，由112 U/g提高至213 U/g，但形成规律基本一致。胞外中性和碱性蛋白酶的活性高低及形成规律均受到氮源的影响。中性蛋白酶活性在氮源培养基中第30天达到最高峰，为10.2 U/g，而在空白培养基上却未检测得显著的峰值。碱性蛋白酶活性受氮源的影响在第10天开始上升，至第50天达到最大值（10 U/g）。在空白培养基上，碱性蛋白酶活性在第50天开始上升，培养结束时达最大值（6.5 U/g）。这3种蛋白酶中，酸性蛋白酶活性与子实体的生长趋势相关性最大。该研究将为蛹虫草子实体生产的营养生理研究提供参考依据。
Abstract:
Taking Cordyceps militaris as materials,the fresh weight of fruit body,pH value in medium,and the activity of extracellular acid protease,neutral protease and alkaline protease during growth of Cordyceps militaris were investigated.The results showed that the activity of acid protease could be affected obviously by nitrogen but not the profile.The highest activity of 112 U/g was promoted to 213 U/g by nitrogen supplement.Both activities and profiles of neutral and alkaline proteases were affected by nitrogen supplement.The highest activity of neutral protease was recorded on the 30th day with 10.2 U/g.And no obvious maximum was detected on substrate without nitrogen supplement.Alkaline protease activity began to rise on the 10th day and reached to the highest (10 U/g) at the 50th day on nitrogen-supplemented substrate.And alkaline protease activity started to rise only on the 50th day and got to highest (6.5 U/g) at the end of the culture.The findings could be helpful for the nutritional physiology researches of Cordyceps militaris.

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备注/Memo

第一作者简介：林群英（1979-），女，博士，助理研究员,现主要从事食用菌栽培与应用等研究工作。E-mail：linqunying1007@126.com.

责任作者：孙晓明（1964-），男，硕士，研究员,现主要从事食用菌栽培与深加工等研究工作。E-mail：sunxm64@163.com。
基金项目：国家“十二五”科技支撑计划资助项目（2013BAD16B07）。

更新日期/Last Update: 2014-09-26