LIANG Li-dong,LI Ming-wen,ZHU Li-guo.Adventitious Bud Induction and Proliferation Studies in Tissue Culture of Lonicera caerulea‘Lanxin’[J].Northern Horticulture,2014,38(17):114-117.
“蓝心忍冬”组织培养中不定芽诱导和增殖研究
- Title:
- Adventitious Bud Induction and Proliferation Studies in Tissue Culture of Lonicera caerulea‘Lanxin’
- 文章编号:
- 1001-0009(2014)17-0114-04
- Keywords:
- Lonicera edulis; adventitious buds; induction; proliferation
- 分类号:
- S663.9
- 文献标志码:
- A
- 摘要:
- 以“蓝心忍冬”无菌茎芽为试材，研究了不同浓度细胞分裂素6-BA、不同浓度生长素NAA和IBA对不定芽诱导和增殖的影响。结果表明：不同浓度6BA、不同浓度NAA和IBA对不定芽的诱导率和增殖系数有显著的影响；不同浓度6-BA显著提高不定芽的诱导率和增殖系数，NAA对不定芽的诱导率和增殖系数优于IBA；综合分析表明，以WPM为基本培养基，当6-BA浓度为0.8 mg/L、NAA浓度为0.15 mg/L时，“蓝心忍冬”不定芽诱导率可以达到90.0%，增殖系数可以达到1.58，且不定芽生长健壮。
- Abstract:
- Taking sterile stem buds of Lonicera caerulea ‘Lanxin’ as material,the effect of different concentrations of cytokinin 6-BA and different concentrations of auxin NAA and IBA on induction and proliferation of adventitious buds were studied.The results showed that there were a significant impact on adventitious bud induction rate and proliferation coefficient in different concentrations of cytokinin 6-BA and different concentrations of auxin NAA and IBA；induction rate and proliferation coefficient of adventitious buds were significantly improved in different concentrations of cytokinin 6-BA,effect on induction and proliferation coefficient of adventitious buds in different concentrations of auxin NAA were better than that in different concentrations of auxin IBA；comprehensive analysis showed that adventitious buds induction rate of Lonicera caerulea ‘Lanxin’ could reach 90%,proliferation coefficient could reach 1.58,further adventitious buds were the robust growth in the case of WPM medium added 6-BA of 0.8 mg/L concentrations and NAA of 0.15 mg/L concentrations.
参考文献/References:
［1］沈海龙.植物组织培养［M］.北京:中国林业出版社,2005.
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备注/Memo
第一作者简介：梁立东（1982-），男，黑龙江青冈人，硕士，工程师，现主要从事树木组织培养及种苗培育和森林培育等研究工作。E-mail:lld_lld@126.com.